Methods and compositions for internally controlled in situ assays

ABSTRACT

The present disclosure relates in some aspects to methods for analyzing a target nucleic acid in a biological sample. In some aspects, the methods involve the use of a set of polynucleotides, including one or more polynucleotides (e.g., a detection padlock probe) for detecting a region of interest and one or more polynucleotides (e.g., a control padlock probe) as an internal control, for analyzing target nucleic acids. In some aspects, the presence, amount, and/or identity of a region of interest in a target nucleic acid is analyzed in situ. Also provided are polynucleotides, sets of polynucleotides, compositions, and kits for use in accordance with the methods.

CROSS-REFERENCE TO RELATED APPLICATION

This application claims priority to U.S. Provisional Patent Application No. 63/139,244, filed Jan. 19, 2021, entitled “METHODS AND COMPOSITIONS FOR INTERNALLY CONTROLLED IN SITU ASSAYS,” which is herein incorporated by reference in its entirety for all purposes.

FIELD

The present disclosure relates in some aspects to methods and compositions for analysis of a target nucleic acid in a sample (e.g., in situ), such as the detection of a region of interest in a polynucleotide.

BACKGROUND

Methods are available for analyzing nucleic acids present in a biological sample, such as a cell or a tissue. For instance, advances in single molecule fluorescent in situ hybridization (smFISH) have enabled nanoscale-resolution imaging of RNA in cells and tissues. However, analysis of short sequences (e.g., single nucleotide polymorphisms (SNPs) or point mutations) on individual transcripts has remained challenging. Improved methods for analyzing nucleic acids present in a biological sample, such as in situ SNP genotyping, are needed. Provided herein are methods and compositions that address such and other needs.

BRIEF SUMMARY

Provided in some embodiments herein are methods and compositions for analyzing a region of interest (e.g., a short sequence of interest such as a single nucleotide of interest) in situ in a biological sample. In some embodiments, a method disclosed herein provides an internal control for in situ analysis of a region of interest.

In some aspects, provided herein is a method for analyzing a region of interest in a target nucleic acid, the method comprising contacting a target nucleic acid with a circularizable control probe (e.g., a control padlock probe) and a detection complex comprising a circularizable detection probe (e.g., a detection padlock probe), wherein the detection complex comprises a sequence complementary to the region of interest and the control padlock probe comprises a sequence complementary to the target nucleic acid at a region that is different from the region of interest. In some embodiments, a circularized detection padlock probe and/or a circularized control padlock probe are formed. In some embodiments, one or more amplification products are generated using the circularized detection padlock probe and/or the circularized control padlock probe as template. In some embodiments, the amplification product of the detection padlock probe and/or the circularized control padlock probe is analyzed. For example, the method may comprise detecting the presence or absence, an amount or level, or a concentration of the amplification product.

In any of the preceding embodiments, the presence of the amplification product of the detection padlock probe and presence of the amplification product of the control padlock probe can indicate the presence of the region of interest in the target nucleic acid.

In any of the preceding embodiments, the absence of the amplification product of the detection padlock probe and the presence of the amplification product of the control padlock probe can indicate the absence of the region of interest in the target nucleic acid.

In any of the preceding embodiments, formation of the circularized control padlock probe may not be dependent on the detection padlock probe hybridizing to the target nucleic acid.

In any of the preceding embodiments, formation of the circularized control padlock probe may not be dependent on the detection complex hybridizing to the target nucleic acid.

In any of the preceding embodiments, formation of the circularized control padlock probe may not be dependent on formation of the circularized detection padlock probe.

In any of the preceding embodiments, the detection padlock probe can comprise the sequence complementary to the region of interest.

In any of the preceding embodiments, the circularized detection padlock probe can be formed using the target nucleic acid as template.

In any of the preceding embodiments, ends of the detection padlock probe can be ligated using the target nucleic acid as template to form the circularized detection padlock, with or without gap filling prior to ligation.

In any of the preceding embodiments, the detection complex can further comprise an anchor.

In any of the preceding embodiments, the anchor can comprise the sequence complementary to the region of interest.

In any of the preceding embodiments, the anchor can comprise a sequence complementary to the target nucleic acid at a region that is different from the region of interest, a sequence that hybridizes to the detection padlock probe, and/or a sequence that hybridizes to the control padlock probe.

In any of the preceding embodiments, the circularized detection padlock probe can be formed using the anchor as template. In some embodiments, ends of the detection padlock probe can be ligated using the anchor as template to form the circularized detection padlock, with or without gap filling prior to ligation.

In any of the preceding embodiments, formation of the circularized control padlock probe can be dependent on the anchor hybridizing to the target nucleic acid.

In any of the preceding embodiments, the circularized control padlock probe can be formed using the anchor as template. In some embodiments, ends of the control padlock probe are ligated using the anchor as template to form the circularized control padlock, with or without gap filling prior to ligation.

In any of the preceding embodiments, the detection complex can further comprise a splint. In some embodiments, the circularized detection padlock probe is formed using the splint as template. In some embodiments, ends of the detection padlock probe are ligated using the splint as template to form the circularized detection padlock, with or without gap filling prior to ligation.

In any of the preceding embodiments, the control padlock probe can hybridize to a sequence of the target nucleic acid that is adjacent to the region of interest.

In any of the preceding embodiments, the anchor can hybridize to a sequence of the target nucleic acid that is adjacent to the region of interest. In some embodiments, the detection padlock probe, the anchor, and the control padlock probe hybridize to adjacent sequences of the target nucleic acid. In some embodiments, the anchor hybridizes to a sequence of the target nucleic acid between the region of interest and the sequence that the control padlock hybridizes to.

In any of the preceding embodiments, the method can further comprise using a primer for rolling circle amplification of the circularized detection padlock probe or the circularized control padlock probe. In some embodiments, the primer comprises a sequence that hybridizes to the target nucleic acid, in addition to a sequence that hybridizes to the circularized detection padlock probe or the circularized control padlock probe.

In any of the preceding embodiments, the method can further comprise using the anchor as a primer for rolling circle amplification of the circularized detection padlock probe or the circularized control padlock probe. In some embodiments, the anchor is used as a primer for rolling circle amplification of the circularized detection padlock probe and a separate primer is provided for rolling circle amplification of the circularized control padlock probe. In some embodiments, the anchor is used as a primer for rolling circle amplification of the circularized control padlock probe and a separate primer is provided for rolling circle amplification of the circularized detection padlock probe.

In any of the preceding embodiments, the region of interest can comprise a single nucleotide of interest, an alternatively spliced region, a deletion, and/or a frameshift. In some embodiments, the single nucleotide of interest is selected from the group consisting of a single-nucleotide polymorphism (SNP), a single-nucleotide variant (SNV), a single-nucleotide substitution, a point mutation, a single-nucleotide deletion, and a single-nucleotide insertion.

In any of the preceding embodiments, the detection complex can comprise a barcode sequence.

In any of the preceding embodiments, the detection padlock probe, the anchor, and/or the splint can comprise a barcode sequence.

In any of the preceding embodiments, the control padlock probe can comprise a barcode sequence.

In any of the preceding embodiments, the detection complex can be a first detection complex and the detection padlock probe can be a first detection padlock probe, wherein the first detection complex comprises a sequence complementary to a first sequence of the region of interest, and the method further comprises: (i) contacting the target nucleic acid with a second detection complex comprising a second detection padlock probe, wherein the second detection complex comprises a sequence complementary to a second sequence of the region of interest, and wherein the first and second sequences of the region of interest are different; (ii) forming a circularized second detection padlock probe; (iii) generating one or more amplification products using the circularized second detection padlock probe; and (iv) detecting the presence or absence of the amplification product of the second detection padlock probe. In some embodiments, the first and second sequences of the region of interest are different at one, two, three, four, five, or more nucleotide positions. In some embodiments, the first and/or second sequences of the region of interest comprise a single nucleotide of interest, an alternatively spliced region, a deletion, and/or a frameshift. In some embodiments, the second sequence of the region of interest is a variant of the first sequence of the region of interest, or vice versa. In some embodiments, the variant comprises a single-nucleotide polymorphism (SNP), a single-nucleotide variant (SNV), a single-nucleotide substitution, a point mutation, a single-nucleotide deletion, or a single-nucleotide insertion.

In any of the preceding embodiments, the first detection complex can comprise a first barcode sequence corresponding to the first sequence of the region of interest, the second detection complex can comprise a second barcode sequence corresponding to the second sequence of the region of interest, and the first and second barcode sequences can be different. In some embodiments, the first detection padlock probe comprises the first barcode sequence, and the second detection padlock probe comprises the second barcode sequence.

In any of the preceding embodiments, the target nucleic acid can be target nucleic acid T1, the region of interest can be region of interest R1, the control padlock probe can be CP1, the detection complex can be C1, the detection padlock probe can be DP1, and the method can further comprise analyzing a region of interest R2, which can be in target nucleic acid T1 or a target nucleic acid T2, wherein R1 and R2 are different. In some embodiments, the method further comprises (i) contacting target nucleic acid T1 or T2 with a control padlock probe CP2 and a detection complex C2 comprising a detection padlock probe DP2, wherein detection complex C2 comprises a sequence complementary to region of interest R2 and control padlock probe CP2 comprises a sequence complementary to target nucleic acid T1 or T2 at a region that is different from region of interest R2; (ii) forming a circularized detection padlock probe DP2 and/or a circularized control padlock probe CP2; (iii) generating one or more amplification products using circularized detection padlock probe DP2 and/or circularized control padlock probe CP2 as template; and (iv) detecting the presence or absence of the amplification product of detection padlock probe DP2 and/or circularized control padlock probe CP2. In some embodiments, detection complex C1 comprises a detection barcode sequence DB1 corresponding to a sequence of region of interest R1, the detection complex C2 comprises a detection barcode sequence DB2 corresponding to a sequence of region of interest R2, control padlock probe CP1 comprises a control barcode sequence CB1, and control padlock probe CP2 comprises a control barcode sequence CB2. In some embodiments, detection padlock probe DP1 comprises detection barcode sequence DB1, and detection padlock probe DP2 comprises detection barcode sequence DB2. In some embodiments, detection barcode sequences DB1 and DB2 are different. In some embodiments, control barcode sequences CB1 and CB2 are the same or different.

In any of the preceding embodiments, the target nucleic acid can be an RNA molecule, and the detection padlock probe and the control padlock probe can be DNA molecules.

In any of the preceding embodiments, the circularized detection padlock probe and/or the circularized control padlock probe can be formed using enzymatic ligation, chemical ligation, template dependent ligation, and/or template independent ligation.

In any of the preceding embodiments, the circularized detection padlock probe and/or the circularized control padlock probe can be formed using enzymatic ligation. In some embodiments, the enzymatic ligation utilizes a ligase. In some embodiments, the ligase is a DNA ligase or an RNA ligase. In some embodiments, the ligase is a T4 RNA ligase, a splintR ligase, a single stranded DNA ligase, or a T4 DNA ligase. In some embodiments, the ligase has a DNA-splinted DNA ligase activity and/or an RNA-splinted DNA ligase activity.

In any of the preceding embodiments, the forming step can comprise ligation performed under conditions permissive for: specific hybridization of the detection padlock probe and the control padlock probe to the target nucleic acid; specific hybridization of the anchor to the target nucleic, the detection padlock probe, and/or the control padlock probe; and/or specific hybridization of the detection padlock probe to other molecules in the detection complex.

In any of the preceding embodiments, the method can further comprise a step of removing molecules that are not specifically hybridized in the detection complex and/or to the target nucleic acid and/or to the control padlock probe prior to and/or after the forming step. In some embodiments, the removing step comprises a wash, e.g., a stringency wash.

In any of the preceding embodiments, the amplification product of the detection padlock probe and/or the circularized control padlock probe can be formed using rolling circle amplification (RCA). In some embodiments, the RCA is a linear RCA, a branched RCA, a dendritic RCA, or any combination thereof.

In any of the preceding embodiments, the detecting step can comprise determining a sequence of the amplification product of the detection padlock probe and/or the circularized control padlock probe. In some embodiments, the detecting step comprises sequencing all or a portion of the amplification products of the detection probe and/or the circularized control probe. In some embodiments, the detecting step comprises in situ hybridization to the amplification products of the detection probe and/or the circularized control probe. In some embodiments, the sequencing comprises sequencing by hybridization, sequencing by ligation, sequencing by synthesis, and/or sequencing by binding. In some embodiments, a barcode sequence in the amplification product of the detection padlock probe and/or the circularized control padlock probe is determined.

In any of the preceding embodiments, the detecting step can comprise imaging the amplification product of the detection padlock probe and/or the circularized control padlock probe.

In any of the preceding embodiments, the target nucleic acid can be an mRNA in a tissue sample, and the detecting step can be performed when the target nucleic acid and/or the amplification product of the detection padlock probe and/or the circularized control padlock probe is in situ in the tissue sample.

In some aspects, provided herein is a method for analyzing a region of interest in a target nucleic acid, the method comprising: (i) contacting a target nucleic acid with a detection padlock probe, an anchor, and a control padlock probe, wherein: (a) the detection padlock probe comprises hybridization regions HR1 and HRa, (b) the anchor comprises hybridization regions HR2 and HRc, (c) the control padlock probe comprises hybridization regions HR3 and HRd, (d) the target nucleic acid comprises adjacent hybridization regions HR1′, HR2′, and HR3′, wherein HR1′ or HR2′ comprises a region of interest, (e) HR1, HR2, and HR3 hybridize to HR1′, HR2′, and HR3′, respectively, (f) HR1 and HR2 comprise a sequence complementary to the region of interest in HR1′ and HR2′, respectively, (g) HRa hybridizes to a hybridization region HRb which is comprised in the anchor or a splint, and (h) HRd is a split region such that sequences at the ends of the control padlock probe hybridize to HRc of the anchor; (ii) ligating the ends of the detection padlock probe to form a first circular probe; (iii) ligating the ends of the control padlock probe using HRc as template to form a second circular probe; (iv) forming a first amplification product using the first circular probe as template; (v) forming a second amplification product using the second circular probe as template; and (vi) analyzing the presence, absence, or an amount of the first and second amplification products.

In some aspects, provided herein is a method for analyzing a region of interest in a target nucleic acid, the method comprising: (i) contacting a target nucleic acid with a detection padlock probe, an anchor, and a control padlock probe, wherein: (a) the detection padlock probe comprises hybridization regions HR1 and HRa, (b) the anchor comprises hybridization regions HR2, HRb, and HRc, (c) the control padlock probe comprises hybridization regions HR3 and HRd, (d) the target nucleic acid comprises adjacent hybridization regions HR1′, HR2′, and HR3′, wherein HR1′ comprises a region of interest, (e) HR1, HR2, and HR3 hybridize to HR1′, HR2′, and HR3′, respectively, (f) HR1 comprises a sequence complementary to the region of interest in HR1′, (g) HRa hybridizes to HRb of the anchor, and (h) HRd is a split region such that sequences at the ends of the control padlock probe hybridize to HRc of the anchor; (ii) ligating the ends of the detection padlock probe using HR1′ as template to form a first circular probe; (iii) ligating the ends of the control padlock probe using HRc as template to form a second circular probe; (iv) forming a first amplification product using the first circular probe as template; (v) forming a second amplification product using the second circular probe as template; and (vi) analyzing the presence, absence, or an amount of the first and second amplification products. in step (iv) HRb of the anchor is used as primer to form the first amplification product, and in step (v) a primer is provided to form the second amplification product.

In any of the preceding embodiments, in step (iv) a primer can be provided to form the first amplification product, and in step (v) HRc of the anchor can be used as primer to form the second amplification product.

In any of the preceding embodiments, the nucleotide complementary to the region of interest can be the 3′ or 5′ end nucleotide of the detection padlock probe.

In some aspects, provided herein is a method for analyzing a region of interest in a target nucleic acid, the method comprising: (i) contacting a target nucleic acid with a detection padlock probe, a splint, an anchor, and a control padlock probe, wherein: (a) the detection padlock probe comprises hybridization regions HR1 and HRa, (b) the splint comprises hybridization region HRb, (c) the anchor comprises hybridization regions HR2 and HRc, (d) the control padlock probe comprises hybridization regions HR3 and HRd, (e) the target nucleic acid comprises adjacent hybridization regions HR1′, HR2′, and HR3′, wherein HR2′ comprises a region of interest, (f) HR1, HR2, and HR3 hybridize to HR1′, HR2′, and HR3′, respectively, (g) HR2 comprises a sequence complementary to the region of interest in HR2′, (h) HRa is a split region such that sequences at the ends of the detection padlock probe hybridize to HRb of the splint, and (i) HRd is a split region such that sequences at the ends of the control padlock probe hybridize to HRc of the anchor; (ii) ligating the anchor and the splint to form a ligated anchor-splint; (iii) ligating the ends of the detection padlock probe using HRb as template to form a first circular probe; (iv) ligating the ends of the control padlock probe using HRc as template to form a second circular probe; (v) forming a first amplification product using the first circular probe as template; (vi) forming a second amplification product using the second circular probe as template; and (vii) analyzing the presence, absence, or an amount of the first and second amplification products. In some embodiments, in step (v) HRb of the anchor-splint is used as primer to form the first amplification product, and in step (vi) a primer is provided to form the second amplification product. In some embodiments, the nucleotide complementary to the region of interest is the 3′ end nucleotide of the anchor. In other embodiments, the nucleotide complementary to the region of interest is the 5′ end nucleotide of the anchor. In some embodiments, in step (v) a primer is provided to form the first amplification product, and in step (vi) HRc of the anchor is used as primer to form the second amplification product.

In some aspects, provided herein is a method for analyzing a region of interest in a target nucleic acid, the method comprising: (i) contacting a target nucleic acid with a detection padlock probe, an anchor, and a control padlock probe, wherein: (a) the detection padlock probe comprises hybridization regions HR1 and HRa, (b) the anchor comprises hybridization regions HR2, HRb, and HRc, (c) the control padlock probe comprises hybridization regions HR3 and HRd, (d) the target nucleic acid comprises adjacent hybridization regions HR1′, HR2′, and HR3′, wherein HR1′ comprises a region of interest at one end of HR1′, (e) HR1, HR2, and HR3 hybridize to HR1′, HR2′, and HR3′, respectively, (f) HR1 comprises a sequence complementary to the region of interest in HR1′ and the complementary nucleotide is at one end of HR1, (g) HRa hybridizes to HRb, and (h) HRd is a split region such that sequences at the ends of the control padlock probe hybridize to HRc of the anchor; (ii) ligating the ends of the detection padlock probe using HRb as template to form a first circular probe; (iii) ligating the ends of the control padlock probe using HRc as template to form a second circular probe; (iv) forming a first amplification product using the first circular probe as template; (v) forming a second amplification product using the second circular probe as template; and (vi) analyzing the presence, absence, or an amount of the first and second amplification products. In some embodiments, in step (iv) HRb of the anchor is used as primer to form the first amplification product, and in step (v) a primer is provided to form the second amplification product. In some embodiments, the nucleotide complementary to the region of interest is the 5′ end nucleotide of HR1 and the detection padlock probe. In other embodiments, the nucleotide complementary to the region of interest is the 3′ end nucleotide of HR1 and the detection padlock probe. In some embodiments, in step (iv) a primer is provided to form the first amplification product, and in step (v) HRc of the anchor is used as primer to form the second amplification product.

In any of the preceding embodiments, the detection padlock probe can comprise a first barcode sequence, wherein the first barcode sequence corresponds to a first sequence in the target nucleic acid. In some embodiments, the first barcode sequence corresponds to the region of interest.

In any of the preceding embodiments, the control padlock probe can comprise a second barcode sequence, wherein the second barcode sequence corresponds to a second sequence in the target nucleic acid, and wherein the second sequence does not comprise the region of interest.

In any of the preceding embodiments, the detection padlock probe can be a first detection padlock probe, the sequence can be complementary to a first sequence of the region of interest, and the method can further comprise: contacting the target nucleic acid with a second detection padlock probe that hybridizes to the target nucleic acid and the anchor, wherein the second detection padlock comprises a sequence complementary to a second sequence of the region of interest, and wherein the first and second sequences of the region of interest are different; forming a circularized second detection padlock probe; generating one or more amplification products using the circularized second detection padlock probe; and detecting the presence or absence of the amplification product of the second detection padlock probe.

In any of the preceding embodiments, the detection padlock probe can be a first detection padlock probe, the anchor can be a first anchor, the sequence can be complementary to a first sequence of the region of interest, and the method can further comprise: contacting the target nucleic acid with a second detection padlock probe and a second anchor that hybridize to each other and to the target nucleic acid, wherein the second anchor comprises a sequence complementary to a second sequence of the region of interest, and wherein the first and second sequences of the region of interest are different; forming a circularized second detection padlock probe; generating one or more amplification products using the circularized second detection padlock probe; and detecting the presence or absence of the amplification product of the second detection padlock probe.

In any of the preceding embodiments, the first and second sequences of the region of interest can be different at one, two, three, four, five, or more nucleotide positions. In some embodiments, the first and/or second sequences of the region of interest comprise a single nucleotide of interest, an alternatively spliced region, a deletion, and/or a frameshift.

In any of the preceding embodiments, the second sequence of the region of interest can be a variant of the first sequence of the region of interest, or vice versa. In some embodiments, the variant comprises a single-nucleotide polymorphism (SNP), a single-nucleotide variant (SNV), a single-nucleotide substitution, a point mutation, a single-nucleotide deletion, or a single-nucleotide insertion.

In any of the preceding embodiments, the target nucleic acid can be in a biological sample. In some embodiments, the region of interest is analyzed in situ in the biological sample.

In some embodiments, the biological sample is a tissue sample. In some embodiments, the tissue sample is an intact tissue sample or a non-homogenized tissue sample. In some embodiments, the biological sample is a processed or cleared tissue sample. In some embodiments, the biological sample is a fixed tissue sample. In some embodiments, the tissue sample is aa formalin-fixed, paraffin-embedded (FFPE) sample. In some embodiments, the tissue sample is a frozen tissue sample. In some embodiments, the tissue sample is a fresh tissue sample.

In any of the preceding embodiments, the biological sample can be embedded in a matrix. In some embodiments, the matrix can comprise a hydrogel. In some embodiments, the hydrogel can be functionalized for attaching biomolecules and/or products thereof from the biological sample.

In any of the preceding embodiments, the biological sample can be a tissue slice between about 1 μm and about 50 μm in thickness. In any of the preceding embodiments, the biological sample can be a tissue slice between about 5 μm and about 35 μm in thickness.

In some aspects, provided herein is a complex comprising a detection padlock probe, an anchor, and a control padlock probe, wherein: (i) the detection padlock probe comprises hybridization regions HR1 and HRa, (ii) the anchor comprises hybridization regions HR2, HRb, and HRc, (iii) the control padlock probe comprises hybridization regions HR3 and HRd, (iv) HR1, HR2, and HR3 hybridize to adjacent hybridization regions HR1′, HR2′, and HR3′, respectively, of a target nucleic acid, wherein HR1′ or HR2′ comprises a region of interest, (v) HR1 and HR2 comprise a sequence complementary to the region of interest in HR1′ and HR2′, respectively, (vi) HRa hybridizes to a hybridization region HRb which is comprised in the anchor or a splint, and (vii) HRd is a split region such that sequences at the ends of the control padlock probe hybridize to HRc of the anchor.

In some aspects, provided herein is a complex comprising a detection padlock probe, an anchor, and a control padlock probe, wherein: (i) the detection padlock probe comprises hybridization regions HR1 and HRa, (ii) the anchor comprises hybridization regions HR2, HRb, and HRc, (iii) the control padlock probe comprises hybridization regions HR3 and HRd, (iv) HR1, HR2, and HR3 hybridize to adjacent hybridization regions HR1′, HR2′, and HR3′, respectively, of a target nucleic acid, wherein HR1′ comprises a region of interest, (v) HR1 comprises a sequence complementary to the region of interest in HR1′, (vi) HRa hybridizes to HRb of the anchor, and (vii) HRd is a split region such that sequences at the ends of the control padlock probe hybridize to HRc of the anchor.

In some aspects, provided herein is a complex comprising a detection padlock probe, an anchor, a splint, and a control padlock probe, wherein: (i) the detection padlock probe comprises hybridization regions HR1 and HRa, (ii) the splint comprises hybridization region HRb, (iii) the anchor comprises hybridization regions HR2 and HRc, (iv) the control padlock probe comprises hybridization regions HR3 and HRd, (v) HR1, HR2, and HR3 hybridize to adjacent hybridization regions HR1′, HR2′, and HR3′, respectively, of a target nucleic acid, wherein HR2′ comprises a region of interest, (vi) HR2 comprises a sequence complementary to the region of interest in HR2′, (vii) HRa is a split region such that sequences at the ends of the detection padlock probe hybridize to HRb of the splint, and (viii) HRd is a split region such that sequences at the ends of the control padlock probe hybridize to HRc of the anchor.

In some aspects, provided herein is a complex comprising a detection padlock probe, an anchor, and a control padlock probe, wherein: (i) the detection padlock probe comprises hybridization regions HR1 and HRa, (ii) the anchor comprises hybridization regions HR2, HRb, and HRc, (iii) the control padlock probe comprises hybridization regions HR3 and HRd, (iv) HR1, HR2, and HR3 hybridize to adjacent hybridization regions HR1′, HR2′, and HR3′, respectively, of a target nucleic acid, wherein HR1′ comprises a region of interest at one end of HR1′, (v) HR1 comprises a sequence complementary to the region of interest in HR1′ and the complementary nucleotide is at one end of HR1, (vi) HRa hybridizes to HRb, and (vii) HRd is a split region such that sequences at the ends of the control padlock probe hybridize to HRc of the anchor.

In any of the preceding embodiments, the complex can further comprise the target nucleic acid. In some embodiments, the target nucleic acid is an mRNA transcript of a gene and any one or more of the detection padlock probe, the anchor, the splint, and the control padlock probe are DNA molecule.

BRIEF DESCRIPTION OF THE DRAWINGS

The drawings illustrate certain embodiments of the features and advantages of this disclosure. These embodiments are not intended to limit the scope of the appended claims in any manner.

FIGS. 1A-1B depict exemplary embodiments of a control complex and a detection complex connected (e.g., via hybridization of a connecting probe) to each other when hybridized on a target nucleic acid.

FIGS. 2A-2C depict exemplary polynucleotides, comprising a control padlock probe, an anchor, a detection padlock probe, a primer, and optionally a splint forming a hybridization complex with a target nucleic acid (e.g., an mRNA), for analyzing a region of interest (e.g., a short sequence or a single nucleotide of interest). The control padlock probe can be ligated when the anchor and the control padlock probe hybridize to the target nucleic acid. The detection padlock probe can be ligated when the region of interest is complementary to a corresponding interrogatory region in the detection complex. The circularized control padlock probe and/or the circularized detection padlock probe can be amplified by rolling circle amplification using the primer and the anchor or the ligated anchor-splint to prime amplification, respectively.

FIGS. 2D-2F depict exemplary polynucleotides, comprising a control padlock probe, an anchor, a detection padlock probe, a primer, and optionally a splint forming a hybridization complex with a target nucleic acid (e.g., an mRNA), for analyzing a region of interest (e.g., a short sequence or a single nucleotide of interest). In some cases, the primer can be used to prime amplification of the circularized detection padlock probe and the anchor or the ligated anchor-splint can be used to prime amplification of the circularized control padlock probe.

DETAILED DESCRIPTION

The practice of the techniques described herein may employ, unless otherwise indicated, conventional techniques and descriptions of organic chemistry, polymer technology, molecular biology (comprising recombinant techniques), cell biology, biochemistry, and sequencing technology, which are within the skill of those who practice in the art. Such conventional techniques comprise polymer array synthesis, hybridization and ligation of polynucleotides, and detection of hybridization using a label. Specific illustrations of suitable techniques can be had by reference to the examples herein. However, other equivalent conventional procedures can, of course, also be used. Such conventional techniques and descriptions can be found in standard laboratory manuals such as Green, et al., Eds. (1999), Genome Analysis: A Laboratory Manual Series (Vols. I-IV); Weiner, Gabriel, Stephens, Eds. (2007), Genetic Variation: A Laboratory Manual; Dieffenbach, Dveksler, Eds. (2003), PCR Primer: A Laboratory Manual; Bowtell and Sambrook (2003), DNA Microarrays: A Molecular Cloning Manual; Mount (2004), Bioinformatics: Sequence and Genome Analysis; Sambrook and Russell (2006), Condensed Protocols from Molecular Cloning: A Laboratory Manual; and Sambrook and Russell (2002), Molecular Cloning: A Laboratory Manual (all from Cold Spring Harbor Laboratory Press); Stryer, L. (1995) Biochemistry (4th Ed.) W. H. Freeman, New York N.Y.; Gait, “Oligonucleotide Synthesis: A Practical Approach” 1984, IRL Press, London; Nelson and Cox (2000), Lehninger, Principles of Biochemistry 3rd Ed., W. H. Freeman Pub., New York, N.Y.; and Berg et al. (2002) Biochemistry, 5^(th) Ed., W. H. Freeman Pub., New York, N.Y., all of which are herein incorporated in their entirety by reference for all purposes.

All publications, comprising patent documents, scientific articles and databases, referred to in this application are incorporated by reference in their entirety for all purposes to the same extent as if each individual publication were individually incorporated by reference. If a definition set forth herein is contrary to or otherwise inconsistent with a definition set forth in the patents, applications, published applications and other publications that are herein incorporated by reference, the definition set forth herein prevails over the definition that is incorporated herein by reference.

The section headings used herein are for organizational purposes only and are not to be construed as limiting the subject matter described.

I. Overview

Fluorescent in situ hybridization (FISH) assays may suffer from low probe specificity and the inability to distinguish single bases, especially when applied to the analysis of short sequences such as SNP genotyping or detection of point mutations. Because FISH assays do not require nucleic acid amplification, the signals are typically low in intensity, resulting in low signal-to-noise ratios and low sensitivity/specificity. FISH assays may also have limited plexity and can be biased, time-consuming, labor-intensive, and/or error-prone for the analysis of short sequences.

In situ sequencing methods are useful for analyzing a target nucleic acid in a cell or tissue sample. For example, a SNAIL (Splint Nucleotide Assisted Intramolecular Ligation) probe set may be used to form a circular construct on an RNA molecule. The circular construct is then amplified (e.g., by rolling circle amplification (RCA)) for in situ sequencing, such as sequencing by ligation (e.g., Sequencing by Dynamic Annealing and Ligation (SEDAL)). See, e.g., U.S. Pat. Pub. 20190055594 and 20210164039, which are hereby incorporated by reference in their entirety. However, detection of short sequences such as SNP genotyping in situ using SNAIL probes is challenging. The present disclosure provides methods and compositions for in situ analysis of short regions of interest, such as a single nucleotide of interest (e.g., SNPs or point mutations), a dinucleotide sequence, or a short sequence of about 5 nucleotides in length.

Provided herein are methods involving the use of a set of polynucleotides for analyzing one or more target nucleic acid(s), such as a target nucleic acid (for example, a messenger RNA) present in a cell or a biological sample, such as a tissue sample. Also provided are polynucleotides, sets of polynucleotides, compositions, kits, systems and devices for use in accordance with the provided methods. In some aspects, the provided methods can be applied to detect, image, quantitate, or determine the presence or absence of one or more target nucleic acid(s) or portions thereof (e.g., presence or absence of sequence variants such as point mutations and SNPs). In some aspects, the provided methods can be applied to detect, image, quantitate, or determine the sequence of one or more target nucleic acid(s), comprising sequence variants such as point mutations and SNPs.

In some aspects, the provided embodiments can be employed for in situ detection and/or sequencing of a target nucleic acid in a cell, e.g., in cells of a biological sample or a sample derived from a biological sample, such as a tissue section on a solid support, such as on a transparent slide.

In some aspects, provided herein are in situ assays using microscopy as a readout, e.g., nucleic acid sequencing, hybridization, or other detection or determination methods involving an optical readout. In some aspects, detection or determination of a sequence of one, two, three, four, five, or more nucleotides of a target nucleic acid is performed in situ in a cell in an intact tissue. In some embodiments, the assay comprises detecting the presence or absence of an amplification product or a portion thereof (e.g., RCA product). In some embodiments, a method for spatially profiling analytes such as the transcriptome or a subset thereof in a biological sample is provided. Methods, compositions, kits, devices, and systems for these in situ assays, comprising spatial genomics and transcriptomics assays, are provided. In some embodiments, a provided method is quantitative and preserves the spatial information within a tissue sample without physically isolating cells or using homogenates.

In some embodiments, through the use of various probe designs (e.g., various padlock probe, splint, anchor, and/or primer oligos), the present disclosure provides methods for high-throughput profiling one or more single nucleotides of interest in a large number of targets in situ, such as transcripts and/or DNA loci, for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms.

In some aspects, the methods disclosed herein involve the use of one or more probes or probe sets that hybridize to a target nucleic acid, such as an RNA molecule, wherein at least one detection probe and at least one control probe are used. Exemplary detection probes and/or control probes may be based on a padlock probe, a gapped padlock probe, a SNAIL (Splint Nucleotide Assisted Intramolecular Ligation) probe set, a PLAYR (Proximity Ligation Assay for RNA) probe set, a PLISH (Proximity Ligation in situ Hybridization) probe set, and RNA-templated ligation probes, or modifications thereof. The specific probe or probe set design can vary. In some embodiments, a primary probe (e.g., a DNA probe that directly binds to an RNA target) that is circularizable is amplified through rolling circle amplification. In some embodiments, the primary probes, such as a padlock probe or a probe set that comprises a padlock probe, contain one or more barcodes. In some embodiments, one or more barcodes are indicative of a sequence in the target nucleic acid, such as a single nucleotide of interest (e.g., SNPs or point mutations), a dinucleotide sequence, or a short sequence of about 5 nucleotides in length.

In some embodiments, polynucleotides (e.g., detection probe, splint and anchor) are designed such that the region complementary to and/or that basepairs with the region of interest on the target is located on a detection padlock probe (e.g., FIG. 2A and FIG. 2C) and/or on an anchor (e.g., FIG. 2B). In some embodiments, the complementary region is internal in a hybridization region (e.g., internal in HR1 in FIG. 2A). For example, the complementary region can be at the 5′ and/or the 3′ end of the detection padlock probe. In some embodiments, the complementary region is at a terminus of a hybridization region (e.g., HR1 in FIG. 2C or HR2 in FIG. 2B), such that a match allows a tri-fork hybridization complex to form and ligation to occur, joining the anchor and the splint (e.g., FIG. 2B) or circularizing the detection padlock probe (e.g., FIG. 2C). In some embodiments, the ligation of the splint and anchor can be a ligation within a tri-fork structure. In some embodiments, the ligation of the ends of a padlock probe can be a ligation within a tri-fork structure.

In some aspects, the provided methods involve analyzing, e.g., detecting or determining, one or more sequences present in the polynucleotides and/or in an amplification product, such as in an amplification product of a circularized padlock probe (e.g., a detection padlock probe and/or a control padlock probe) which comprises one or more barcode sequences. In some embodiments, the analysis comprises determining the sequence of all or a portion of the amplification product. In some embodiments, the analysis comprises detecting a sequence present in the amplification product. In some embodiments, the sequence of all or a portion of the amplification product is indicative of the identity of a region (e.g., a single nucleotide) of interest in a target nucleic acid. In some embodiments, the analysis can be used to correlate a sequence detected in an amplification product to a detection padlock probe or a control padlock probe (e.g., via a barcode). In some embodiments, the detection of a sequence in an amplification product can provide information regarding the location of an associated target nucleic acid (e.g., hybridized by the control padlock probe and/or a detection padlock probe) in a sample. In some embodiments, due to amplification of one or more polynucleotides (e.g., a detection padlock probe and/or a control padlock probe), particular sequences present in the amplification product or complementary sequences thereof can be detected even when a polynucleotide is present at low levels before the amplification. For example, the number of copies of the barcode sequence(s) and/or a complementary sequence thereof is increased by virtue of the amplification of a probe comprising the barcode sequence(s) and/or complementary sequence thereof, thereby enabling specific and sensitive detection of a signal indicative of the identity of a short region (e.g., a single nucleotide) of interest in a target nucleic acid. In particular embodiments, the amplification product is an in situ rolling circle amplification (RCA) product of a circularized padlock probe.

In some embodiments, the methods involve the use of a set of polynucleotides, such as a set of three or more polynucleotides. In some aspects, the set of polynucleotides comprises two polynucleotides (e.g., a padlock probe and an anchor, such as those in the detection complex of FIG. 2A and FIG. 2C) for analyzing a target nucleic acid. In some aspects, the set of polynucleotides comprises three polynucleotides (e.g., a padlock probe, a splint, and an anchor, such as those in the detection complex of FIG. 2B) for analyzing a target nucleic acid. In some aspects, the set of polynucleotides comprises four polynucleotides (e.g., a padlock probe, a splint, an anchor, and a primer capable of hybridizing to the padlock probe and/or a target nucleic acid) for analyzing a target nucleic acid.

In some aspects, the set of polynucleotides comprises three polynucleotides (e.g., a detection padlock probe, an anchor, and a control padlock probe such as those in the detection complex of FIG. 2A, FIG. 2C, FIG. 2D, and FIG. 2F) for analyzing a target nucleic acid. In some aspects, the set of polynucleotides comprises four polynucleotides (e.g., a detection padlock probe, an anchor, a control padlock probe, and a primer, such as those in the detection complex of FIG. 2A, FIG. 2C, FIG. 2D, and FIG. 2F) for analyzing a target nucleic acid. In some aspects, the set of polynucleotides comprises five polynucleotides (e.g., a detection padlock probe, an anchor, a splint, a control padlock probe, and a primer, such as those in the detection complex of FIG. 2B and FIG. 2E) for analyzing a target nucleic acid.

In some aspects, the polynucleotides (e.g., the set of three or more polynucleotides) contain hybridization regions (HRs) that hybridize to target sites in the target nucleic acids (e.g., DNA or mRNA in a cell), and also HRs that hybridize to one or more other polynucleotides in the set of polynucleotides. In some aspects, one or more polynucleotides of the set of polynucleotides are used in an extension reaction. In some aspects, one or more polynucleotides of the set of polynucleotides are amplified. In some aspects, the provided methods can be applied for various applications, comprising for in situ analysis, comprising in situ detection (e.g., based on hybridization such as sequential hybridization) and/or sequencing of target nucleic acids and multiplexed nucleic acid analysis. In some aspects, the provided methods can be for in situ detection and/or identification of a region (e.g., single nucleotide) of interest in target nucleic acids.

In some embodiments, provided herein are methods for assessing one or more target nucleic acids, such as a plurality of different mRNAs, in a biological sample, such as a cell or a tissue sample (such as a tissue section). In some aspects, the provided methods are employed for in situ analysis of target nucleic acids, for example for in situ sequencing or multiplexed analysis in intact tissues or a sample with preserved cellular or tissue structure. In some aspects, the provided methods can be used to detect or determine the identity or amount in situ of single nucleotides of interest in target nucleic acids, for instance of single nucleotide polymorphisms of genes of interest.

In some aspects, the provided methods involve a step of contacting, or hybridizing, one or more polynucleotides, such as a detection padlock probe, a control padlock probe, a splint, and/or an anchor, to a cell or a sample containing a target nucleic acid with a region (e.g., single nucleotide) of interest in order to form a hybridization complex. In some aspects, the provided methods comprise one or more steps of ligating the polynucleotides, for instance of ligating the anchor and the splint to form a ligated anchor-splint and/or the ends of the detection padlock probe and/or the control padlock probe to form a circularized probe. In some aspects, the provided methods involve a step of amplifying one of the polynucleotides (e.g., a padlock probe or a circularized probe produced therefrom), to generate an amplification product. In some aspects, the provided methods involve a step of detecting and/or determining the sequence of all or a portion of the amplification product (for example, of one or more barcodes contained in the amplification product) and/or one or more of the polynucleotides with or without amplification, for instance any barcodes contained therein. In some aspects, the provided methods involve performing one or more of the steps described herein, simultaneously and/or sequentially.

Particulars of the steps of the methods can be carried out as described herein, for example in Sections II-VI; and/or using any suitable processes and methods for carrying out the particular steps.

II. Samples and Sample Processing

Target nucleic acid molecules can be derived from or analyzed in any specific type of cell and/or a specific sub-cellular region, e.g., from cytosol, from cell nuclei, from mitochondria, from microsomes, and more generally, from any other compartment, organelle, or portion of a cell. Examples comprise DNA analytes such as genomic DNA, methylated DNA, specific methylated DNA sequences, fragmented DNA, mitochondrial DNA, and RNA/DNA hybrids. In some embodiments, a method disclosed herein may comprise a step for increasing accessibility of a nucleic acid for binding, e.g., a denaturation step to opening up DNA in a cell for hybridization by a probe. For example, proteinase K treatment may be used to free up DNA with proteins bound thereto.

Examples of target nucleic acid molecules also comprise RNA analytes such as various types of coding and non-coding RNA. Examples of the different types of RNA analytes comprise messenger RNA (mRNA), ribosomal RNA (rRNA), transfer RNA (tRNA), microRNA (miRNA), pre-mRNA, and viral RNA. RNA analytes can be obtained from cells or cellular compartments (e.g., nucleus). The RNA can be a transcript (e.g., present in a tissue section). The RNA can be small (e.g., less than 200 nucleic acid bases in length) or large (e.g., RNA greater than 200 nucleic acid bases in length). Small RNAs mainly comprise 5.8S ribosomal RNA (rRNA), 5S rRNA, transfer RNA (tRNA), microRNA (miRNA), small interfering RNA (siRNA), small nucleolar RNA (snoRNAs), Piwi-interacting RNA (piRNA), tRNA-derived small RNA (tsRNA), and small rDNA-derived RNA (srRNA). The RNA can be double-stranded RNA or single-stranded RNA. The RNA can be circular RNA. The RNA can be a bacterial rRNA (e.g., 16s rRNA or 23s rRNA).

In some embodiments, a target nucleic acid molecule comprises a polymorphic locus, e.g., a location in the nucleotide sequence of the alleles of a gene of a diploid organism that may be occupied by different nucleotides. The difference may be the result of a SNP, a point mutation, a nucleotide insertion, or a nucleotide deletion. In some embodiments, a target nucleic acid molecule comprises a SNP, e.g., a polynucleotide that differs from another polynucleotide at a particular locus by virtue of a single nucleotide exchange. A polynucleotide may contain numerous SNPs each occurring at a different locus. For example, exchanging one A for one C, G or T at a particular locus in the sequence of a polynucleotide constitutes a SNP. SNPs can occur in coding and non-coding regions of a gene, and may be in DNA or an RNA, such as an mRNA transcript. Those in coding regions are of primary interest because such SNPs can cause changes in the phenotype, i.e., a detectable physical difference in an individual compared to the general population. Detectable physical differences comprise, without limitation, a difference in susceptibility to a particular disease or disorder or a difference in response to a therapeutic regime used to treat or prevent a disease or disorder. In some embodiments, a target nucleic acid molecule comprises a point mutation, e.g., a change at a single locus in a polynucleotide strand. The change may be the deletion of a nucleotide, the addition of a nucleotide or the substitution of one nucleotide for another. In some embodiments, a target nucleic acid molecule may include a DNA locus that is a non-coding region.

In some embodiments, a sequence of interest in a target nucleic acid molecule is one nucleotide (e.g., a SNP or point mutation), two nucleotides (e.g., CpG and GpC dinucleotides), three nucleotides, four nucleotides, five nucleotides, or longer. In some embodiments, the sequence of interest is 5 or fewer, 4 or fewer, 3 or fewer, or 2 or fewer nucleotides in length.

In some aspects, the provided methods are used to analyze target nucleic acids present in a sample or a specimen, such as in a cell or a tissue sample. In some examples, the sample or specimen is deposited on a surface. In some aspects, the target nucleic acids are present in a sample or a specimen among a plurality of different nucleic acids. In some aspects, the sample is a biological sample, is derived from a biological sample, or is a biological sample that was subject to various processes or manipulations. In some aspects, the provided methods also involve processing or manipulating the sample, for instance before any hybridization, ligation, amplification, and/or detection steps. In some aspects, such processing or manipulating facilitates the analysis of the target nucleic acids. In some aspects, such processing or manipulating preserves the spatial information of the target nucleic acid within the sample or specimen.

In some aspects, the provided methods are used to analyze target nucleic acids in an intact tissue or non-homogenized tissue. In some embodiments, the target nucleic acid is in a cell in the tissue. Tissue specimens suitable for use with the methods described herein comprise any type of tissue specimens collected from living or dead subjects, such as, e.g., biopsy specimens and autopsy specimens, of which comprise, but are not limited to, epithelium, muscle, connective, and nervous tissue. Tissue specimens may be collected and processed using the methods described herein and subjected to microscopic analysis immediately following processing, or may be preserved and subjected to microscopic analysis at a future time, e.g., after storage for an extended period of time. In some embodiments, the methods described herein may be used to preserve tissue specimens in a stable, accessible, and fully intact form for future analysis. In some embodiments, the methods described herein may be used to analyze a previously-preserved or stored tissue specimen. In some embodiments, the intact tissue comprises brain tissue such as visual cortex slices. In some embodiments, the intact tissue is a thin slice with a thickness of 5-20 μm, comprising, but not limited to, e.g., 5-18 μm, 5-15 μm, or 5-10 μm. In some embodiments, the intact tissue is a thick slice with a thickness of 50-200 μm, comprising, but not limited to, e.g., 50-150 μm, 50-100 μm, or 50-80 μm.

A variety of steps can be performed to prepare or process a biological sample for and/or during analysis. Except where indicated otherwise, the preparative or processing steps described below can generally be combined in any manner and in any order to appropriately prepare or process a particular sample for and/or analysis.

A. Tissue Sectioning

A biological sample can be harvested from a subject (e.g., via surgical biopsy, whole subject sectioning) or grown in vitro on a growth substrate or culture dish as a population of cells, and prepared for analysis as a tissue slice or tissue section. Grown samples may be sufficiently thin for analysis without further processing steps. Alternatively, grown samples, and samples obtained via biopsy or sectioning, can be prepared as thin tissue sections using a mechanical cutting apparatus such as a vibrating blade microtome. As another alternative, in some embodiments, a thin tissue section can be prepared by applying a touch imprint of a biological sample to a suitable substrate material.

The thickness of the tissue section can be a fraction of (e.g., less than 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, or 0.1) the maximum cross-sectional dimension of a cell. However, tissue sections having a thickness that is larger than the maximum cross-section cell dimension can also be used. For example, cryostat sections can be used, which can be, e.g., 10-20 micrometers thick.

More generally, the thickness of a tissue section typically depends on the method used to prepare the section and the physical characteristics of the tissue, and therefore sections having a wide variety of different thicknesses can be prepared and used. For example, the thickness of the tissue section can be at least 0.1, 0.2, 0.3, 0.4, 0.5, 0.7, 1.0, 1.5, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, 15, 20, 30, 40, or 50 micrometers. Thicker sections can also be used if desired or convenient, e.g., at least 70, 80, 90, or 100 micrometers or more. Typically, the thickness of a tissue section is between 1-100 micrometers, 1-50 micrometers, 1-30 micrometers, 1-25 micrometers, 1-20 micrometers, 1-15 micrometers, 1-10 micrometers, 2-8 micrometers, 3-7 micrometers, or 4-6 micrometers, but as mentioned above, sections with thicknesses larger or smaller than these ranges can also be analysed.

Multiple sections can also be obtained from a single biological sample. For example, multiple tissue sections can be obtained from a surgical biopsy sample by performing serial sectioning of the biopsy sample using a sectioning blade. Spatial information among the serial sections can be preserved in this manner, and the sections can be analysed successively to obtain three-dimensional information about the biological sample.

B. Freezing

In some embodiments, the biological sample (e.g., a tissue section as described above) can be prepared by deep freezing at a temperature suitable to maintain or preserve the integrity (e.g., the physical characteristics) of the tissue structure. Such a temperature can be, e.g., less than −20° C., or less than −25° C., −30° C., −40° C., −50° C., −60° C., −70° C., −80° C. −90° C., −100° C., −110° C., −120° C., −130° C., −140° C., −150° C., −160° C., −170° C., −180° C., −190° C., or −200° C. The frozen tissue sample can be sectioned, e.g., thinly sliced, onto a substrate surface using any number of suitable methods. For example, a tissue sample can be prepared using a chilled microtome (e.g., a cryostat) set at a temperature suitable to maintain both the structural integrity of the tissue sample and the chemical properties of the nucleic acids in the sample. Such a temperature can be, e.g., less than −15° C., less than −20° C., or less than −25° C.

C. Formalin Fixation and Paraffin Embedding

In some embodiments, the biological sample can be prepared using formalin-fixation and paraffin-embedding (FFPE), which are established methods. In some embodiments, cell suspensions and other non-tissue samples can be prepared using formalin-fixation and paraffin-embedding. Following fixation of the sample and embedding in a paraffin or resin block, the sample can be sectioned as described above. Prior to analysis, the paraffin-embedding material can be removed from the tissue section (e.g., deparaffinization) by incubating the tissue section in an appropriate solvent (e.g., xylene) followed by a rinse (e.g., 99.5% ethanol for 2 minutes, 96% ethanol for 2 minutes, and 70% ethanol for 2 minutes).

D. Fixation

As an alternative to formalin fixation described above, a biological sample can be fixed in any of a variety of other fixatives to preserve the structure of the sample prior to, during, and/or after analysis. For example, a sample can be fixed via immersion in ethanol, methanol, acetone, paraformaldehyde (PFA)-Triton, and combinations thereof.

In some embodiments, acetone fixation is used with fresh frozen samples, which can include, but are not limited to, cortex tissue, mouse olfactory bulb, human brain tumor, human post-mortem brain, and breast cancer samples. When acetone fixation is performed, pre-permeabilization steps (described below) may not be performed. Alternatively, acetone fixation can be performed in conjunction with permeabilization steps.

In some aspects, the methods comprise fixing an intact tissue. In some aspects, the methods are performed on fixed intact tissue. Generally, fixing or fixation involves preserving biological material (e.g., tissues, cells, organelles, molecules, etc.) from decay and/or degradation. Fixation may be accomplished using any convenient protocol. Fixation can comprise contacting the sample with a fixation reagent (i.e., a reagent that contains at least one fixative). Samples can be contacted by a fixation reagent for a wide range of times, which can depend on the temperature, the nature of the sample, and on the fixative(s). For example, a sample can be contacted by a fixation reagent for 24 or less hours, 18 or less hours, 12 or less hours, 8 or less hours, 6 or less hours, 4 or less hours, 2 or less hours, 60 or less minutes, 45 or less minutes, 30 or less minutes, 25 or less minutes, 20 or less minutes, 15 or less minutes, 10 or less minutes, 5 or less minutes, or 2 or less minutes.

A sample can be contacted by a fixation reagent at various temperatures, depending on the protocol and the reagent used. For example, in some instances a sample can be contacted by a fixation reagent at a temperature ranging from −22° C. to 55° C., where specific ranges of interest comprise, but are not limited to 50 to 54° C., 40 to 44° C., 35 to 39° C., 28 to 32° C., 20 to 26° C., 0 to 6° C., and −18 to −22° C. In some instances, a sample can be contacted by a fixation reagent at a temperature of −20° C., 4° C., room temperature (22-25° C.), 30° C., 37° C., 42° C., or 52° C.

Any convenient fixation reagent can be used. Common fixation reagents comprise crosslinking fixatives, precipitating fixatives, oxidizing fixatives, mercurials, and the like. Crosslinking fixatives chemically join two or more molecules by a covalent bond and a wide range of cross-linking reagents can be used. Examples of suitable cross liking fixatives comprise but are not limited to aldehydes (e.g., formaldehyde, also commonly referred to as “paraformaldehyde” and “formalin”; glutaraldehyde; etc.), imidoesters, NHS (N-Hydroxysuccinimide) esters, and the like. Examples of suitable precipitating fixatives comprise but are not limited to alcohols (e.g., methanol, ethanol, etc.), acetone, acetic acid, etc. In some embodiments, the fixative is formaldehyde (i.e., paraformaldehyde or formalin). A suitable final concentration of formaldehyde in a fixation reagent is 0.1 to 10%, 1-8%, 1-4%, 1-2%, 3-5%, or 3.5-4.5%, comprising about 1.6% for 10 minutes. In some embodiments the sample is fixed in a final concentration of 4% formaldehyde (as diluted from a more concentrated stock solution, e.g., 38%, 37%, 36%, 20%, 18%, 16%, 14%, 10%, 8%, 6%, etc.). In some embodiments the sample is fixed in a final concentration of 10% formaldehyde. In some embodiments the sample is fixed in a final concentration of 1% formaldehyde. In some embodiments, the fixative is glutaraldehyde. A suitable concentration of glutaraldehyde in a fixation reagent is 0.1 to 1%. A fixation reagent can contain more than one fixative in any combination. For example, in some embodiments the sample is contacted with a fixation reagent containing both formaldehyde and glutaraldehyde. In addition to the fixation methods described, tissue may be paraffin-embedded (FFPE), a frozen, or processed fresh.

In some embodiments, the methods provided herein comprises one or more post-fixing (also referred to as postfixation) steps. In some embodiments, one or more post-fixing step is performed after contacting a sample with a polynucleotide disclosed herein, e.g., one or more of the polynucleotides disclosed in Section III, such as an anchor, a splint, a primer, and/or a circular or padlock probe. In some embodiments, one or more post-fixing step is performed after a hybridization complex (e.g., as disclosed in Section III) is formed in a sample. In some embodiments, one or more post-fixing step is performed prior to a ligation reaction disclosed herein, e.g., as disclosed in Section IV.

In some embodiments, one or more post-fixing step is performed after contacting a sample with a labelling agent (e.g., an antibody or antigen binding fragment thereof) for a non-nucleic acid analyte such as a protein analyte. The labelling agent can comprise a nucleic acid molecule (e.g., a reporter oligonucleotide) comprising a sequence corresponding to the labelling agent and therefore corresponds to (e.g., uniquely identifies) the protein analyte. In some embodiments, the labelling agent can comprise a nucleic acid molecule (e.g., reporter oligonucleotide) comprising one or more barcode sequences.

A post-fixing step may be performed using any suitable fixation reagent disclosed herein, for example, 3% (w/v) paraformaldehyde in DEPC-PBS.

E. Tissue Permeabilization

In some embodiments, a biological sample can be permeabilized to facilitate transfer of analytes out of the sample, and/or to facilitate transfer of species (such as probes) into the sample. If a sample is not permeabilized sufficiently, the amount of analyte available for analysis may be too low to enable adequate analysis. Conversely, if the tissue sample is too permeable, the relative spatial relationship of the analytes within the tissue sample can be lost. Hence, a balance between permeabilizing the tissue sample enough to obtain good signal intensity while still maintaining the spatial resolution of the analyte distribution in the sample is desirable.

In general, a biological sample can be permeabilized by exposing the sample to one or more permeabilizing agents. Suitable agents for this purpose include, but are not limited to, organic solvents (e.g., acetone, ethanol, and methanol), cross-linking agents (e.g., paraformaldehyde), detergents (e.g., saponin, Triton X-100™ or Tween-20™), and enzymes (e.g., trypsin, proteases). In some embodiments, the biological sample can be incubated with a cellular permeabilizing agent to facilitate permeabilization of the sample. Additional methods for sample permeabilization are described, for example, in Jamur et al., Method Mol. Biol. 588:63-66, 2010, the entire contents of which are incorporated herein by reference. Any suitable method for sample permeabilization can generally be used in connection with the samples described herein.

In some embodiments, where a diffusion-resistant medium is used to limit migration of analytes or other species during the analytical procedure, the diffusion-resistant medium can include at least one permeabilization reagent. For example, the diffusion-resistant medium can include wells (e.g., micro-, nano-, or picowells) containing a permeabilization buffer or reagents. In some embodiments, where the diffusion-resistant medium is a hydrogel, the hydrogel can include a permeabilization buffer. In some embodiments, the hydrogel is soaked in permeabilization buffer prior to contacting the hydrogel with a sample. In some embodiments, the hydrogel or other diffusion-resistant medium can contain dried reagents or monomers to deliver permeabilization reagents when the diffusion-resistant medium is applied to a biological sample. In some embodiments, the diffusion-resistant medium, (i.e. hydrogel) is covalently attached to a solid substrate (i.e. an acrylated glass slide).

In some embodiments, permeabilization solution can be delivered to a sample through a porous membrane. In some embodiments, a porous membrane is used to limit diffusive analyte losses, while allowing permeabilization reagents to reach a sample. Membrane chemistry and pore size can be manipulated to minimize analyte loss. In some embodiments, the porous membrane may be made of glass, silicon, paper, hydrogel, polymer monoliths, or other material. In some embodiments, the material may be naturally porous. In some embodiments, the material may have pores or wells etched into solid material. In some embodiments, the permeabilization reagents are flowed through a microfluidic chamber or channel over the porous membrane. In some embodiments, the flow controls the sample's access to the permeabilization reagents. In some embodiments, a porous membrane is sandwiched between a substrate and the sample, wherein permeabilization solution is applied over the porous membrane. The permeabilization reagents diffuse through the pores of the membrane and into the tissue.

In some embodiments, the biological sample can be permeabilized by adding one or more lysis reagents to the sample. Examples of suitable lysis agents include, but are not limited to, bioactive reagents such as lysis enzymes that are used for lysis of different cell types, e.g., gram positive or negative bacteria, plants, yeast, mammalian, such as lysozymes, achromopeptidase, lysostaphin, labiase, kitalase, lyticase, and a variety of other commercially available lysis enzymes.

Other lysis agents can additionally or alternatively be added to the biological sample to facilitate permeabilization. For example, surfactant-based lysis solutions can be used to lyse sample cells. Lysis solutions can include ionic surfactants such as, for example, sarcosyl and sodium dodecyl sulfate (SDS). More generally, chemical lysis agents can include, without limitation, organic solvents, chelating agents, detergents, surfactants, and chaotropic agents.

In some embodiments, the biological sample can be permeabilized by non-chemical permeabilization methods. Non-chemical permeabilization methods are known in the art. For example, non-chemical permeabilization methods that can be used include, but are not limited to, physical lysis techniques such as electroporation, mechanical permeabilization methods (e.g., bead beating using a homogenizer and grinding balls to mechanically disrupt sample tissue structures), acoustic permeabilization (e.g., sonication), and thermal lysis techniques such as heating to induce thermal permeabilization of the sample.

In some aspects, the methods provided herein comprise permeabilizing the tissue. In some embodiments, the methods are performed using permeabilized tissue. In some aspects, permeabilization comprises a process of rendering the cells (cell membranes etc.) of a sample permeable to experimental reagents such as nucleic acid probes, antibodies, chemical substrates, etc. Any convenient method and/or reagent for permeabilization can be used. Suitable permeabilization reagents comprise detergents (e.g., Saponin, Triton X-100, Tween-20, etc.), organic fixatives (e.g., acetone, methanol, ethanol, etc.), enzymes, etc. Detergents can be used at a range of concentrations. For example, 0.001%-1% detergent, 0.05%-0.5% detergent, or 0.1%-0.3% detergent can be used for permeabilization (e.g., 0.1% Saponin, 0.2% tween-20, 0.1-0.3% triton X-100, etc.). In some embodiments, methanol on ice for at least 10 minutes is used to permeabilize.

In some embodiments, a sample can be contacted by a permeabilization reagent for a wide range of times, which can depend on the temperature, the nature of the sample, and on the permeabilization reagent(s). For example, a sample can be contacted by a permeabilization reagent for 24 or more hours, 24 hours or less, 18 hours or less, 12 hours or less, 8 hours or less, 6 hours or less, 4 hours or less, 2 hours or less, 60 minutes or less, 45 minutes or less, 30 minutes or less, 25 minutes or less, 20 minutes or less, 15 minutes or less, 10 minutes or less, 5 minutes or less, or 2 minutes or less. In some embodiments, a sample can be contacted by a permeabilization reagent at various temperatures, depending on the protocol and the reagent used. For example, in some instances a sample can be contacted by a permeabilization reagent at a temperature ranging from −82° C. to 55° C., where specific ranges of interest comprise, but are not limited to: 50 to 54° C., 40 to 44° C., 35 to 39° C., 28 to 32° C., 20 to 26° C., 0 to 6° C., −18 to −22° C., and −78 to −82° C. In some instances a sample can be contacted by a permeabilization reagent at a temperature of −80° C., −20° C., 4° C., room temperature (in some examples, less than 30° C., or 22-25° C.), 30° C., 37° C., 42° C., or 52° C.

In some embodiments, a sample is contacted with an enzymatic permeabilization reagent. Enzymatic permeabilization reagents that permeabilize a sample by partially degrading extracellular matrix or surface proteins that hinder the permeation of the sample by assay reagents. Contact with an enzymatic permeabilization reagent can take place at any point after fixation and prior to target detection. In some instances, the enzymatic permeabilization reagent is proteinase K, a commercially available enzyme.

In such cases, the sample is contacted with proteinase K prior to contact with a post fixation reagent. Proteinase K treatment (i.e., contact by proteinase K; also commonly referred to as “proteinase K digestion”) can be performed over a range of times at a range of temperatures, over a range of enzyme concentrations that are empirically determined for each cell type or tissue type under investigation. For example, a sample can be contacted by proteinase K for 30 minutes or less, 25 minutes or less, 20 minutes or less, 15 minutes or less, 10 minutes or less, 5 minutes or less, or 2 minutes or less. A sample can be contacted by 1 pg/ml or less, 2 pg/ml or less, 4 pg/ml or less, 8 pg/ml or less, 10 pg/ml or less, 20 pg/ml or less, 30 pg/ml or less, 50 pg/ml or less, or 100 pg/ml or less proteinase K. In some embodiments, a sample can be contacted by proteinase K at a temperature ranging from 2° C. to 55° C., where specific ranges of interest comprise, but are not limited to: 50 to 54° C., 40 to 44° C., 35 to 39° C., 28 to 32° C., 20 to 26° C., and 0 to 6° C. In some instances, a sample can be contacted by proteinase K at a temperature of 4° C., room temperature (in some examples, less than 30° C., or 22-25° C.), 30° C., 37° C., 42° C., or 52° C. In some embodiments, a sample is not contacted with an enzymatic permeabilization reagent. In some embodiments, a sample is not contacted with proteinase K. In some aspects, contact of an intact tissue with at least a fixation reagent and a permeabilization reagent results in the production of a fixed and permeabilized tissue.

F. Embedding

As an alternative to paraffin embedding described above, a biological sample can be embedded in any of a variety of other embedding materials to provide structural substrate to the sample prior to sectioning and other handling steps. In general, the embedding material is removed prior to analysis of tissue sections obtained from the sample. Suitable embedding materials include, but are not limited to, waxes, resins (e.g., methacrylate resins), epoxies, and agar.

In some embodiments, the biological sample can be embedded in a hydrogel matrix. Embedding the sample in this manner typically involves contacting the biological sample with a hydrogel such that the biological sample becomes surrounded by the hydrogel. For example, the sample can be embedded by contacting the sample with a suitable polymer material, and activating the polymer material to form a hydrogel. In some embodiments, the hydrogel is formed such that the hydrogel is internalized within the biological sample.

In some embodiments, the biological sample is immobilized in the hydrogel via cross-linking of the polymer material that forms the hydrogel. Cross-linking can be performed chemically and/or photochemically, or alternatively by any other hydrogel-formation method known in the art.

In some embodiments, a method disclosed herein comprises de-crosslinking a reversibly cross-linked biological sample. The de-crosslinking does not need to be complete. In some embodiments, only a portion of crosslinked molecules in the reversibly cross-linked biological sample are de-crosslinked.

The composition and application of the hydrogel-matrix to a biological sample typically depends on the nature and preparation of the biological sample (e.g., sectioned, non-sectioned, type of fixation). As one example, where the biological sample is a tissue section, the hydrogel-matrix can include a monomer solution and an ammonium persulfate (APS) initiator/tetramethylethylenediamine (TEMED) accelerator solution. As another example, where the biological sample consists of cells (e.g., cultured cells or cells disassociated from a tissue sample), the cells can be incubated with the monomer solution and APS/TEMED solutions. For cells, hydrogel-matrix gels are formed in compartments, including but not limited to devices used to culture, maintain, or transport the cells. For example, hydrogel-matrices can be formed with monomer solution plus APS/TEMED added to the compartment to a depth ranging from about 0.1 μm to about 2 mm.

Additional methods and aspects of hydrogel embedding of biological samples are described for example in Chen et al., Science 347(6221):543-548, 2015, the entire contents of which are incorporated herein by reference.

In some embodiments, the methods disclosed comprise embedding the sample in a matrix-forming material, e.g., in a manner to substantially retain the relative three-dimensional spatial relationship of a plurality of nucleic acids in the sample. In some embodiments, the methods disclosed comprise making a three dimensional matrix of nucleic acids. In some embodiments, nucleic acids are covalently bound into a matrix or into or to a matrix material. The nucleic acids may be co-polymerized with the matrix material or cross-linked to the matrix material or both. According to one aspect, a plurality of nucleic acid sequences of certain length, such as DNA or RNA sequences are part of a three-dimensional copolymer. The nucleic acids may then be amplified and detected and/or analyzed (e.g., sequenced) in situ, i.e. within the matrix. The three-dimensional matrix of nucleic acids provides, in a certain aspect, an information storage medium where the nucleic acids, i.e. a sequence of one or more nucleotides, represent stored information which can be read within the three-dimensional matrix. Matrix forming materials may comprise polyacrylamide, cellulose, alginate, polyamide, cross-linked agarose, cross-linked dextran, cross-linked polyethylene glycol, disulfide cross-linked polyacrylamide, agarose, alginate, polyvinyl alcohol, polyethylene glycol (PEG)-diacrylate, PEG-acrylate, PEG-thiol, PEG-azide, PEG-alkyne, other acrylates, chitosan, hyaluronic acid, collagen, fibrin, gelatin, or elastin, e.g., as described in U.S. Pat. No. 10,138,509, herein specifically incorporated by reference. The matrix forming materials can form a matrix by polymerization and/or cross-linking of the matrix forming materials using methods specific for the matrix forming materials and known methods, reagents and conditions.

In some aspects, the matrix is sufficiently optically transparent or otherwise has optical properties suitable for detection, imaging and/or sequencing, for example, as described herein and/or using standard high-throughput sequencing chemistries and deep three dimensional imaging for high throughput information readout. Exemplary high-throughput sequencing chemistries that utilize fluorescence imaging comprise ABI SoLiD (Life Technologies), in which a sequencing primer on a template is ligated to a library of fluorescently labeled nonamers with a cleavable terminator. After ligation, the beads are then imaged using four color channels (FITC, Cy3, Texas Red and Cy5). The terminator is then cleaved off leaving a free-end to engage in the next ligation-extension cycle. After all dinucleotide combinations have been determined, the images are mapped to the color code space to determine the specific base calls per template. The workflow is achieved using an automated fluidics and imaging device (i.e., SoLiD 5500 W Genome Analyzer, ABI Life Technologies). Another sequencing platform uses sequencing by synthesis, in which a pool of single nucleotide with a cleavable terminator is incorporated using DNA polymerase. After imaging, the terminator is cleaved and the cycle is repeated. The fluorescence images are then analyzed to call bases for each DNA amplicons within the flow cell (HiSeq, Illumina).

In some embodiments, the methods disclosed comprise preparing a biological tissue specimen for microscopic analysis, e.g., a process that maintains the 3-D integrity of the tissue by embedding it in a hydrogel, and making molecules of interest accessible for optical probing and molecular labeling while allowing undesired molecules such as lipids to be washed away. See Chung et al., “CLARITY for mapping the nervous system,” Nature Methods 10 (2013) and U.S. Pat. No. 10,545,075, herein specifically incorporated by reference. In some embodiments, the methods disclosed herein comprise fixing a biological tissue specimen obtained from a mammal by contacting the biological tissue specimen with a fixation agent and a plurality of hydrogel subunits, thereby cross-linking the hydrogel subunits to biomolecules within the biological tissue specimen to produce biomolecule-bound hydrogel subunits. In some embodiments, the methods further comprise polymerizing the biomolecule-bound hydrogel subunits to form a hydrogel-embedded biological tissue specimen. In some embodiments, the methods further comprise electrophoresing the hydrogel-embedded biological tissue specimen to remove a plurality of cellular components from the specimen and form a cleared hydrogel-embedded biological tissue specimen.

In some embodiments, the methods disclosed comprise embedding the sample in a hydrogel. The hydrogel-tissue chemistry described comprises covalently attaching nucleic acids to in situ synthesized hydrogel for tissue clearing, enzyme diffusion, and multiple-cycle sequencing. In some embodiments, a hydrogel or hydrogel network comprises a network of polymer chains that are water-insoluble, sometimes found as a colloidal gel in which water is the dispersion medium. In other words, hydrogels are a class of polymeric materials that can absorb large amounts of water without dissolving. Hydrogels can contain over 99% water and may comprise natural or synthetic polymers, or a combination thereof.

Hydrogels also possess a degree of flexibility very similar to natural tissue, due to their significant water content. A detailed description of suitable hydrogels may be found in published U.S. Patent Application 2010/0055733, herein specifically incorporated by reference. As used herein, the terms “hydrogel subunits” or “hydrogel precursors” mean hydrophilic monomers, prepolymers, or polymers that can be crosslinked, or “polymerized”, to form a three-dimensional (3D) hydrogel network. Without being bound by any scientific theory, it is believed that this fixation of the biological specimen in the presence of hydrogel subunits crosslinks the components of the specimen to the hydrogel subunits, thereby securing molecular components in place, preserving the tissue architecture and cell morphology.

In some embodiments, the embedding comprises copolymerizing the one or more amplicons with acrylamide, for example, to form a copolymer which contains more than one type of subunit. A copolymer encompasses polymers which comprise two, three, four, five, or six types of subunits.

G. Staining and Immunohistochemistry (IHC)

To facilitate visualization, biological samples can be stained using a wide variety of stains and staining techniques. In some embodiments, for example, a sample can be stained using any number of stains and/or immunohistochemical reagents. One or more staining steps may be performed to prepare or process a biological sample for an assay described herein or may be performed during and/or after an assay. In some embodiments, the sample can be contacted with one or more nucleic acid stains, membrane stains (e.g., cellular or nuclear membrane), cytological stains, or combinations thereof. In some examples, the stain may be specific to proteins, phospholipids, DNA (e.g., dsDNA, ssDNA), RNA, an organelle or compartment of the cell. The sample may be contacted with one or more labeled antibodies (e.g., a primary antibody specific for the analyte of interest and a labeled secondary antibody specific for the primary antibody). In some embodiments, cells in the sample can be segmented using one or more images taken of the stained sample.

In some embodiments, the stain is performed using a lipophilic dye. In some examples, the staining is performed with a lipophilic carbocyanine or aminostyryl dye, or analogs thereof (e.g, DiI, DiO, DiR, DiD). Other cell membrane stains may include FM and RH dyes or immunohistochemical reagents specific for cell membrane proteins. In some examples, the stain may include but is not limited to, acridine orange, acid fuchsin, Bismarck brown, carmine, coomassie blue, cresyl violet, DAPI, eosin, ethidium bromide, acid fuchsine, haematoxylin, Hoechst stains, iodine, methyl green, methylene blue, neutral red, Nile blue, Nile red, osmium tetroxide, ruthenium red, propidium iodide, rhodamine (e.g., rhodamine B), or safranine, or derivatives thereof. In some embodiments, the sample may be stained with haematoxylin and eosin (H&E).

The sample can be stained using hematoxylin and eosin (H&E) staining techniques, using Papanicolaou staining techniques, Masson's trichrome staining techniques, silver staining techniques, Sudan staining techniques, and/or using Periodic Acid Schiff (PAS) staining techniques. PAS staining is typically performed after formalin or acetone fixation. In some embodiments, the sample can be stained using Romanowsky stain, including Wright's stain, Jenner's stain, Can-Grunwald stain, Leishman stain, and Giemsa stain. The sample can also be subjected to staining of cell nuclei, such as by fluorescent dyes including DAPI, Hoechst 33258, olivomycin, and/or acriflavin and/or nonfluorescent staining.

In some embodiments, biological samples can be destained. Methods of destaining or discoloring a biological sample are known in the art, and generally depend on the nature of the stain(s) applied to the sample. For example, in some embodiments, one or more immunofluorescent stains are applied to the sample via antibody coupling. Such stains can be removed using techniques such as cleavage of disulfide linkages via treatment with a reducing agent and detergent washing, chaotropic salt treatment, treatment with antigen retrieval solution, and treatment with an acidic glycine buffer. Methods for multiplexed staining and destaining are described, for example, in Bolognesi et al., J. Histochem. Cytochem. 2017; 65(8): 431-444, Lin et al., Nat Commun. 2015; 6:8390, Pirici et al., J. Histochem. Cytochem. 2009; 57:567-75, and Glass et al., J. Histochem. Cytochem. 2009; 57:899-905, the entire contents of each of which are incorporated herein by reference.

H. Isometric Expansion

In some embodiments, a biological sample embedded in a hydrogel can be isometrically expanded. Isometric expansion methods that can be used include hydration, a preparative step in expansion microscopy, as described in Chen et al., Science 347(6221):543-548, 2015.

Isometric expansion can be performed by anchoring one or more components of a biological sample to a gel, followed by gel formation, proteolysis, and swelling. Isometric expansion of the biological sample can occur prior to immobilization of the biological sample on a substrate, or after the biological sample is immobilized to a substrate. In some embodiments, the isometrically expanded biological sample can be removed from the substrate prior to contacting the substrate with probes disclosed herein.

In general, the steps used to perform isometric expansion of the biological sample can depend on the characteristics of the sample (e.g., thickness of tissue section, fixation, cross-linking), and/or the analyte of interest (e.g., different conditions to anchor RNA, DNA, and protein to a gel).

In some embodiments, proteins in the biological sample are anchored to a swellable gel such as a polyelectrolyte gel. An antibody can be directed to the protein before, after, or in conjunction with being anchored to the swellable gel. DNA and/or RNA in a biological sample can also be anchored to the swellable gel via a suitable linker. Examples of such linkers include, but are not limited to, 6-((Acryloyl)amino) hexanoic acid (Acryloyl-X SE) (available from ThermoFisher, Waltham, Mass.), Label-IT Amine (available from MirusBio, Madison, Wis.) and Label X (described for example in Chen et al., Nat. Methods 13:679-684, 2016, the entire contents of which are incorporated herein by reference).

Isometric expansion of the sample can increase the spatial resolution of the subsequent analysis of the sample. The increased resolution in spatial profiling can be determined by comparison of an isometrically expanded sample with a sample that has not been isometrically expanded.

In some embodiments, a biological sample is isometrically expanded to a size at least 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.1×, 3.2×, 3.3×, 3.4×, 3.5×, 3.6×, 3.7×, 3.8×, 3.9×, 4×, 4.1×, 4.2×, 4.3×, 4.4×, 4.5×, 4.6×, 4.7×, 4.8×, or 4.9× its non-expanded size. In some embodiments, the sample is isometrically expanded to at least 2× and less than 20× of its non-expanded size.

I. Substrate Attachment

In general, a substrate can be used to provide support to a biological sample, particularly, for example, a thin tissue section. Accordingly, a “substrate” is a support that is insoluble in aqueous liquid and which allows for positioning of biological samples, analytes, features, and/or probes on the substrate.

A wide variety of different substrates can be used for the foregoing purposes. In general, a substrate can be any suitable support material. Exemplary substrates include, but are not limited to, glass, modified and/or functionalized glass, hydrogels, films, membranes, plastics (including e.g., acrylics, polystyrene, copolymers of styrene and other materials, polypropylene, polyethylene, polybutylene, polyurethanes, Teflon″, cyclic olefins, polyimides etc.), nylon, ceramics, resins, Zeonor, silica or silica-based materials including silicon and modified silicon, carbon, metals, inorganic glasses, optical fiber bundles, and polymers, such as polystyrene, cyclic olefin copolymers (COCs), cyclic olefin polymers (COPs), polypropylene, polyethylene and polycarbonate.

The substrate can also correspond to a flow cell. Flow cells can be formed of any of the foregoing materials, and can include channels that permit reagents, solvents, features, and molecules to pass through the cell.

Among the examples of substrate materials discussed above, polystyrene is a hydrophobic material suitable for binding negatively charged macromolecules because it normally contains few hydrophilic groups. For nucleic acids immobilized on glass slides, by increasing the hydrophobicity of the glass surface the nucleic acid immobilization can be increased. Such an enhancement can permit a relatively more densely packed formation (e.g., provide improved specificity and resolution).

In some embodiments, a substrate is coated with a surface treatment such as poly(L)-lysine. Additionally or alternatively, the substrate can be treated by silanation, e.g. with epoxy-silane, amino-silane, and/or by a treatment with polyacrylamide.

III. Polynucleotides and Hybridization Complexes

In some aspects, the methods provided herein comprise use of a set of polynucleotides, for instance a detection padlock probe, a control padlock probe, an anchor, and/or a splint. In some aspects, the polynucleotides further comprise a primer. In some aspects, any or all of the polynucleotides are DNA molecules. In some aspects, the polynucleotides are used to analyze a target nucleic acid, e.g., messenger RNA in a cell or a biological sample. In some aspects, the polynucleotides are used to analyze, e.g., detect and/or identify, a region of interest in a target nucleic acid.

A. Target Nucleic Acid

In some aspects, a target nucleic acid disclosed herein comprises any polynucleotide nucleic acid molecule (e.g., DNA molecule; RNA molecule, modified nucleic acid, etc.) for assessment in accordance with the provided embodiments, such as a polynucleotide present in a cell. In some embodiments, the target nucleic acid is a coding RNA (e.g., mRNA). The target may, in some embodiments, be a single RNA molecule. In other embodiments, the target may be at least one RNA molecule, e.g., a group of 2, 3, 4, 5, 6 or more RNA molecules. These RNA molecules may differ in molecule type, and/or may differ in sequence. In some embodiments, the target nucleic acid is, for example, a non-coding RNA (e.g., tRNA, rRNA, microRNA (miRNA), mature miRNA or immature miRNA). In some embodiments, the target nucleic acid is a splice variant of an RNA molecule (e.g., mRNA, pre-mRNA, etc.) in the context of a cell. A suitable target nucleic acid can therefore be an unspliced RNA (e.g., pre-mRNA, mRNA), a partially spliced RNA, or a fully spliced RNA, etc. Target nucleic acids of interest may be variably expressed, i.e., have a differing abundance, within a cell population, wherein the methods of the present disclosure allow profiling and comparison of the expression levels of nucleic acids, comprising but not limited to, RNA transcripts, in individual cells. A target nucleic acid can also be a DNA molecule, e.g., a denatured genomic, viral, plasmid, etc. For example, the methods can be used to detect copy number variants, e.g., in a cancer cell population in which a target nucleic acid is present at different abundance in the genome of cells in the population; a virus-infected cells to determine the virus load and kinetics, and the like.

In some aspects, the methods provided herein are used to analyze a target nucleic acid, e.g., a messenger RNA molecule. In some embodiments, the target nucleic acid is present in a cell in a tissue, for instance in a tissue sample or tissue section. In some embodiments, the tissue sample is an intact tissue sample or a non-homogenized tissue sample. In some embodiments, the tissue sample is a fresh tissue sample. In some embodiments, the tissue has previously been processed, e.g., fixed, embedded, frozen, or permeabilized using any of the steps and/or protocols described in Section II.

In some embodiments, the target nucleic acid comprises one or more hybridization regions (HRs). Exemplary HR regions are depicted in FIGS. 2A-2F and are labelled as HR3′, HR2′, and HR1′. The HRs refer to regions that are complementary or sufficiently complementary to a different nucleic acid sequence (for example, to another HR present in one or more of the polynucleotides) to form complexes via, e.g., Watson-Crick base pairing. In some embodiments, the target nucleic acid comprises three adjacent HRs. In some embodiments, the three HRs are immediately adjacent to one another. In other embodiments, any of the two HRs are separated by a gap, for instance a gap of 1, 2, 3, 4, or 5 nucleotides. In some embodiments, the target nucleic acid comprises, from 5′ end to 3′ end, HR1′, HR2′, and HR3′. In some embodiments, the target nucleic acid comprises, from 5′ end to 3′ end, HR3′, HR2′, and HR1′.

In some embodiments, any of the HRs contained in the target nucleic acid are between or between about 5 and 200 nucleotides in length. In some embodiments, any of the HRs contained in the target nucleic acid are between or between about 5 and 100 nucleotides in length. In some embodiments, any of the HRs contained in the target nucleic acid are between or between about 5 and 40 nucleotides in length. In some embodiments, the HR are between or between about 5 and 15 nucleotides in length. In some embodiments, the HR are between or between about 15 and 20 nucleotides in length. In some embodiments, the HR are between or between about 20 and 25 nucleotides in length. In some embodiments, the HR are between or between about 25 and 30 nucleotides in length. In some embodiments, the HR are between or between about 30 and 35 nucleotides in length. In some embodiments, the HR are between or between about 25 and 30 nucleotides in length. In some embodiments, the HR are between or between about 35 and 40 nucleotides in length.

In some embodiments, the region of interest of the target nucleic acid comprises one, two, three, four, five, or more nucleotide positions. In some embodiments, the region of interest can comprise a single nucleotide of interest, an alternatively spliced region, a deletion, and/or a frameshift. In some embodiments, the single nucleotide of interest is selected from the group consisting of a single-nucleotide polymorphism (SNP), a single-nucleotide variant (SNV), a single-nucleotide substitution, a point mutation, a single-nucleotide deletion, and a single-nucleotide insertion. In some embodiments, provided herein are methods and compositions for analyzing two or more sequences of a region of interest (e.g., a first and second sequence of the region of interest). In some embodiments, the first and second sequences of the region of interest are different at one, two, three, four, five, or more nucleotide positions. In some embodiments, the first and/or second sequences of the region of interest comprise a single nucleotide of interest, an alternatively spliced region, a deletion, and/or a frameshift. In some embodiments, the second sequence of the region of interest is a variant of the first sequence of the region of interest, or vice versa. In some embodiments, the variant comprises a single-nucleotide polymorphism (SNP), a single-nucleotide variant (SNV), a single-nucleotide substitution, a point mutation, a single-nucleotide deletion, or a single-nucleotide insertion.

In some embodiments, HR1′ or HR2′ contains the region of interest. In some embodiments, the HR containing the region of interest is between or between about 5 and 40 nucleotides in length. In some embodiments, the HR containing the region of interest is between or between about 5 and 15 nucleotides in length. In some embodiments, the HR containing the region of interest is between or between about 15 and 20 nucleotides in length. In some embodiments, the HR containing the region of interest is between or between about 20 and 25 nucleotides in length. In some embodiments, the HR containing the region of interest is between or between about 25 and 30 nucleotides in length. In some embodiments, the HR containing the region of interest is between or between about 30 and 35 nucleotides in length. In some embodiments, the HR containing the region of interest is between or between about 25 and 30 nucleotides in length. In some embodiments, the HR containing the region of interest is between or between about 35 and 40 nucleotides in length.

In some embodiments, the region of interest comprises one of the five terminal nucleotide positions of the HR. In other embodiments, the region of interest is internal in an HR (e.g., the region of interest may be located within HR1′, as shown in FIGS. 2A and 2D). In some embodiments, the region of interest comprises the fifth terminal nucleotide position of the HR, wherein the 3′ or 5′ end nucleotide occupies the first terminal nucleotide position. In some embodiments, the region of interest comprises the fourth terminal nucleotide position of the HR. In some embodiments, the region of interest comprises the third terminal nucleotide position of the HR. In some embodiments, the region of interest comprises the second terminal nucleotide position of the HR (e.g., the nucleotide immediately 3′ to the 5′ end nucleotide of HR2′, or HR1′, or the nucleotide immediately 5′ to the 3′ end nucleotide of HR2′ or HR1′). In some embodiments, the region of interest comprises the terminus of the HR. In some embodiments, the region of interest comprises the 5′ end nucleotide of the HR (e.g., the 5′ end nucleotide of HR2′). In other embodiments, the region of interest comprises the 3′ end nucleotide of the HR (e.g., the 3′ end nucleotide of HR2′ or HR1′).

In some embodiments, the region of interest is a single nucleotide of interest. In some embodiments, the single nucleotide of interest is at one of the five terminal nucleotide positions of the HR. In other embodiments, the single nucleotide of interest is internal in an HR (e.g., the single nucleotide of interest may be located within HR1′, as shown in FIGS. 2A and 2D). In some embodiments, the single nucleotide of interest is at the fifth terminal nucleotide position of the HR, wherein the 3′ or 5′ end nucleotide occupies the first terminal nucleotide position. In some embodiments, the single nucleotide of interest is at the fourth terminal nucleotide position of the HR. In some embodiments, the single nucleotide of interest is at the third terminal nucleotide position of the HR. In some embodiments, the single nucleotide of interest is at the second terminal nucleotide position of the HR (e.g., the nucleotide immediately 3′ to the 5′ end nucleotide of HR2′ or HR1′, or the nucleotide immediately 5′ to the 3′ end nucleotide of HR2′ or HR1′). In some embodiments, the single nucleotide of interest is at the terminus of the HR. In some embodiments, the single nucleotide of interest is the 5′ end nucleotide of the HR (e.g., the 5′ end nucleotide of HR2′ or HR1′). In other embodiments, the single nucleotide of interest is the 3′ end nucleotide of the HR (e.g., the 3′ end nucleotide of HR2′ or HR1′).

In some embodiments, the region of interest comprises the 5′ end nucleotide of an HR (e.g., HR2′ or HR1′) and is immediately adjacent to the 3′ end nucleotide of the adjacent HR (HR1′ or HR2′, respectively) contained in the target nucleic acid. In other embodiments, the region of interest comprises the 3′ end nucleotide of an HR (e.g., HR2′ or HR1′) and is immediately adjacent to the 5′ end nucleotide of the adjacent HR (HR1′ or HR2′, respectively) contained in the target nucleic acid, as is depicted in FIGS. 2A-2F.

B. Probe Oligos and Oligo Sets

In some embodiments, provided herein is a complex comprising a control complex and a detection complex connected to each other when hybridized on a target nucleic acid, where the complexes can serve as internal controls for each other in an in situ assay. The control complex and/or the detection complex con comprise one or more polynucleotides disclosed herein.

In accordance with the provided embodiments, the polynucleotides (e.g., the detection and control probes (e.g., detection and control padlock probes), anchor, and optionally, splint and/or primer) also comprise HRs and are designed to permit hybridization to HRs contained in other polynucleotides and/or in the target nucleic acid. In some aspects, the polynucleotides (e.g., the detection and control padlock probes, anchor, and optionally, splint and/or primer) may be provided at the same time, provided in combinations sequentially in any order, or each of the polynucleotides may be provided sequentially in any order. In some aspects, the polynucleotides for use in the methods provided herein are designed to form a hybridization complex, for instance one containing the target nucleic acid and each of the polynucleotides. In some aspects, the polynucleotides are designed such that ligation and/or amplification of one or more polynucleotides is dependent on the identity of the region of interest. In some aspects, such design permits the analysis, e.g., detection or identification, of the region of interest or the analysis, e.g., detection or sequencing, of a sequence indicative of the identity of the region of interest. In some embodiments, a plurality of control and detection padlock probes, anchors, and optionally, splints and/or primers can be used to target various regions of interest (e.g., various SNPs or SNP locations).

The detection and control probes provided herein can each be a circularizable probes or probe set (e.g., a padlock probe). A circularizable probe or probe set can be provided in one or more probe parts (e.g., as a single nucleic acid molecule, or as two or more nucleic acid molecules) that can be ligated together to form a circularized probe. In some embodiments, the circularizable probe or probe set (e.g., padlock probe) can be provided as one or more nucleic acid molecules (e.g., comprising two or more segments and/or a splint). Since the probe may be provided in one or more parts it follows that there may be more than one ligation junction. In other words, one or more probe parts may each comprise, or generate (i.e. by cleavage or extension), ligatable 5′ and 3′ ends, and the probe as a whole may comprise, or generate, one or more ligatable 5′ ends and one or more ligatable 3′ ends. For example, a padlock probe could be provided in two or more parts.

FIGS. 1A-1B show exemplary embodiments of a control complex and a detection complex. In some embodiments, the control complex comprises a control padlock probe that hybridizes to a region of a target nucleic acid, and the detection complex comprises a detection padlock probe that hybridizes to another region of the target nucleic acid, wherein a connecting probe hybridizes to sequences within the detection padlock probe and the control padlock probe, as shown in FIG. 1A. In some embodiments, the connecting probe comprises a single polynucleotide. In some embodiments, the connecting probe comprises two or more polynucleotides. In some examples, the connecting probe comprises an anchor (as shown in FIG. 1B). In some cases, the connecting probe may comprise an anchor and a splint. In some embodiments, the connecting probe hybridizes to sequences within both the detection padlock probe and the control padlock probe only after template-mediated ligation (e.g., ligation of an additional polynucleotide such as a splint polynucleotide). In some embodiments, hybridization of the components of the connecting probe (e.g., anchor and splint) is stabilized by ligation of said components. In some embodiments, the control complex comprises a control padlock probe and the detection complex comprises a detection padlock probe, wherein the connecting probe is an anchor that hybridizes to a region of the target nucleic acid between the region hybridized to the control padlock probe and the region hybridized to the detection padlock probe. In some embodiments, the control padlock probe binds to a region of the same target nucleic acid as the detection padlock probe. In some aspects, along this same target nucleic acids, the control padlock probe can bind upstream or downstream (5′ or 3′) of the region designed to be hybridized by the detection padlock probe. In some cases, detecting the control padlock probe can indicate the location of the target nucleic acid in the sample, even if the detection probe is not detected.

In some aspects, the polynucleotides (e.g., the detection and control padlock probes, anchor, and optionally, splint and/or primer) may be contacted with the target nucleic acid at the same time, in combinations sequentially in any order, or each of the polynucleotides may be contacted with the target nucleic acid sequentially in any order.

FIGS. 2A-2F show exemplary embodiments of a detection complex and control complex provided herein. In some aspects, the polynucleotides of a detection complex and control complex provided herein comprise four polynucleotides: a control padlock, a primer, a detection padlock probe, and an anchor. In some embodiments, the primer optionally comprises a sequence that anneals to the target nucleic acid (indicated by a dashed line in FIGS. 2A-2F). In some aspects, the polynucleotides of a detection complex and control complex probe further comprise fifth polynucleotide that is a splint (e.g., a DNA splint). In some aspects, any or all of the polynucleotides are DNA molecules. In some aspects, the methods and compositions enable single nucleotide discrimination in situ. Thus, the region of interest shown in FIGS. 2A-2F can be a single nucleotide. In some embodiments as shown in FIGS. 2A-2C, the target nucleic acid comprises from 5′ end to 3′ end: a region HR1′ capable of hybridizing to a region HR1 of the detection padlock probe, a region HR2′ capable of hybridizing to a region HR2 of the anchor, and a region HR3′ capable of hybridizing to a region HR3 of the control padlock. In this 5′ to 3′ configuration, the anchor or ligated anchor/splint can serve as a primer for amplification of the circularized detection padlock probe, and an additional primer can be used to amplify the circularized control padlock probe. In some embodiments, as shown in FIGS. 2D-2F, the opposite 5′ to 3′ configuration can be used. Thus, in some embodiments, as shown in FIGS. 2D-2F, the target nucleic acid comprises from 3′ end to 5′ end: a region HR1′ capable of hybridizing to a region HR1 of the detection padlock probe, a region HR2′ capable of hybridizing to a region HR2 of the anchor, and a region HR3′ capable of hybridizing to a region HR3 of the control padlock. In this 3′ to 5′ configuration, the anchor can serve as a primer for the circularized control padlock probe, and an additional primer can be used to amplify the circularized detection padlock probe.

In some embodiments, as shown in FIGS. 2A and 2D, a sequence comprised by HR1′ of the target nucleic acid is used as a template for circularization of the detection probe. In some embodiments, the detection padlock probe comprises an interrogatory region within HR1 that hybridizes to a region of interest within the target nucleotide sequence HR1′. In some embodiments, the region of interest is short (e.g., 1, 2, 3, or 4 nucleotides). In some embodiments, the region of interest is a nucleotide of interest. In some embodiments, the interrogatory region is located at the 5′ or 3′ end of the detection padlock probe. In some embodiments, the interrogatory region is split between the 5′ and 3′ ends of the detection padlock probe. In some embodiments, the control padlock probe comprises hybridization regions HR3 and HRd, the padlock probe comprises hybridization regions HR1 and HRa, the anchor comprises hybridization regions HR2, HRb, and HRc, the target nucleic acid comprises adjacent hybridization regions HR1′, HR2′, and HR3′, wherein HR1′ comprises a region of interest, HR1 hybridizes to HR1′, wherein HR1 comprises a nucleotide complementary to the region of interest in HR1′ and the complementary nucleotide is at a terminus of the detection padlock probe, HR2 hybridizes to HR2′, HR3 hybridizes to HR3′, HRa hybridizes to HRb, and HRc hybridizes to HRd.

FIGS. 2A and 2D show exemplary embodiments of a detection complex and control complex, wherein a sequence comprised by the target nucleotide sequence HR1′ is used as a template for circularization of the detection probe. As shown in FIG. 2A, the detection padlock probe can comprise an interrogatory region located at the 5′ or 3′ end of the detection padlock probe, wherein the interrogatory region can hybridize to a region of interest within HR1′. In some embodiments, the region of interest is a single nucleotide of interest (e.g., C), and the interrogatory region is a single interrogatory nucleotide (e.g., G) that base pairs with the single nucleotide of interest. Base pairing between the interrogatory nucleotide and the nucleotide of interest allows the target nucleic acid to serve as a template for circularization of the detection padlock probe. Hybridization of the anchor and control padlock probe allows for circularization of the control padlock probe. Circularization of both the detection padlock probe and the control padlock probe allows for rolling circle amplification of both the control and detection padlock probes, thereby allowing detection of both the control padlock barcode and the detection padlock barcode. If the nucleotide of interest does not match (i.e., basepair with) the interrogatory nucleotide of the detection padlock probe, then the control padlock probe but not the detection padlock probe will be circularized and amplified by rolling circle amplification. Thus, mismatch between the nucleotide of interest and the interrogatory interest results in detection of the control padlock barcode only.

In some embodiments, the method comprises contacting a target nucleic acid with a control complex and one or more detection complexes. In some embodiments, there is provided a first detection complex for detecting a first nucleotide of interest (e.g., a SNP) and a second detection complex for detecting an alternate nucleotide of interest (e.g., an alternate SNP). For example, a region of interest may comprise one of two variants, the first nucleotide of interest is C, and the alternate nucleotide of interest is A. However, the methods and compositions provided herein can discriminate between any two nucleotides. For example, the second detection complex can comprise a second detection padlock barcode and an alternate interrogatory nucleotide (e.g., T) capable of basepairing with an alternate nucleotide of interest (e.g., A). Circularization of both the second detection padlock probe and the control padlock probe allows for rolling circle amplification of both the control and second detection padlock probes, thereby allowing detection of both the control padlock barcode and the second detection padlock barcode. In some embodiments, the presence of the first SNP results in detection of the control padlock barcode and the first detection padlock barcode, while the presence of the alternate SNP results in detection of the control padlock barcode and the second detection padlock barcode. In some embodiments, a sample may comprise both variants, such the control padlock barcode(s), the first detection padlock barcode, and the second detection padlock barcode is detected. In some embodiments, detection of the control barcode only indicates that neither the SNP detected by the first detection padlock probe nor the alternate SNP detected by the second detection padlock probe is present in the target nucleic acid.

In some embodiments as shown in FIG. 2A, the target nucleic acid comprises from 5′ end to 3′ end: a region HR1′ capable of hybridizing to a region HR1 of the detection padlock probe, a region HR2′ capable of hybridizing to a region HR2 of the anchor, and a region HR3′ capable of hybridizing to a region HR3 of the control padlock. In this 5′ to 3′ configuration, the anchor can serve as a primer for amplification of the circularized detection padlock probe by hybridization of HRb to a sequence HRa in the detection padlock probe, and an additional primer can be used to amplify the circularized control padlock probe. In some embodiments, as shown in FIG. 2D, the opposite 5′ to 3′ configuration can be used. Thus, in some embodiments, as shown in FIG. 2D, the target nucleic acid comprises from 3′ end to 5′ end: a region HR1′ capable of hybridizing to a region HR1 of the detection padlock probe, a region HR2′ capable of hybridizing to a region HR2 of the anchor, and a region HR3′ capable of hybridizing to a region HR3 of the control padlock. In this 3′ to 5′ configuration, the anchor can serve as a primer for the circularized control padlock probe by hybridization of HRc to sequence HRd of the control padlock probe, and an additional primer can be used to amplify the circularized detection padlock probe.

In some embodiments, as shown in FIGS. 2B and 2E, the anchor comprises a terminal interrogatory region within HR2 (e.g., an interrogatory region at the 3′ end or 5′ end of the anchor, as shown in FIG. 2B and FIG. 2E, respectively) that hybridizes to a region of interest at one end of HR2′ (e.g., at the 5′ end or 3′ end of HR2, as shown in FIG. 2B and FIG. 2E, respectively), and the complex further comprises a splint (e.g., a DNA splint) that can be used as template to circularize the detection padlock. In some embodiments, the terminal nucleotides of the anchor and the splint can be ligated using the DNA padlock as template to create a ligated anchor-splint. In some embodiments, the ligation of the terminal nucleotides of the anchor and the splint is dependent on hybridization of the interrogatory region of the anchor to the region of interest in the target nucleic acid. In some embodiments, the ligated anchor-splint, but not the splint alone, may function as a primer for rolling circle amplification, allowing specific detection of the target region of interest by the detection complex. In some embodiments, the splint is short (e.g., fewer than 10 nucleotides in length) and hybridization/stability for padlock circularization is dependent on the ligation of the anchor and the splint. In some embodiments, the control padlock probe comprises hybridization regions HR3 and HRd, the padlock probe comprises hybridization regions HR1 and HRa, the anchor comprises hybridization regions HR2 and HRc, the splint comprises hybridization region HRb, the target nucleic acid comprises adjacent hybridization regions HR1′, HR2′, and HR3′, wherein HR2′ comprises a region of interest at a terminus of HR2′, HR2 hybridizes to HR2′, wherein HR2 comprises a nucleotide complementary to the region of interest in HR2′ and the complementary nucleotide is at a terminus of HR1, HR1 hybridizes to HR1′, HR3 hybridizes to HR3′, HRa hybridizes to HRb, and HRc hybridizes to HRd.

In some aspects, the methods disclosed herein involve ligation of one or more oligonucleotides in a detection hybridization complex comprising a target nucleic acid, for example, in a tri-fork hybridization complex formed by at least three nucleic acid (e.g., DNA) strands and a target molecule (e.g., DNA or RNA), e.g., as shown in FIGS. 2B and 2E, and the two ends of a DNA padlock probe can be ligated using a DNA splint as template to circularize the padlock, and terminal nucleotides of the anchor and the splint can be ligated using the DNA padlock as template to create a ligated anchor-splint. In some embodiments, the ligated anchor-splint may function as a primer for rolling circle amplification. In some embodiments, the ligated anchor-splint provides stability for the ligation of the detection padlock probe to form a circular probe and/or to help stabilize the hybridization of the detection padlock/circularized detection padlock probe on the target for in situ amplification. In some embodiments, the polynucleotides can be designed such that subsequent ligation and/or amplification is dependent on the kinetics of any of the polynucleotides (e.g., padlock probe, splint, anchor) and whether one or more earlier hybridization steps and/or the ligation step(s) occur.

FIGS. 2B and 2E show exemplary embodiments of a control complex and a detection complex wherein the anchor comprises a terminal interrogatory region within HR2 (e.g., an interrogatory region at the 5′ end or 3′ end of the anchor) that hybridizes to a region of interest at one end of HR2′ (e.g., at the 3′ end or 5′ end of HR2), and the complex further comprises a splint (e.g., a DNA splint) that can be used as template to circularize the detection padlock probe. In some embodiments, the interrogatory region is a single interrogatory nucleotide (e.g., G) that basepairs with a single nucleotide of interest (e.g., C). In some embodiments, the terminal nucleotides of the anchor and the splint can be ligated using the DNA padlock as template to create a ligated anchor-splint. In some embodiments, the ligated anchor-splint, but not the splint alone, may function as a primer for rolling circle amplification of the circularized detection padlock probe, allowing specific detection of the target region of interest by the detection complex. Thus, circularization of both the detection padlock probe and the control padlock probe allows for rolling circle amplification of both the control and detection padlock probes, thereby allowing detection of both the control padlock barcode and the detection padlock barcode. If the nucleotide of interest does not match (i.e., basepair with) the interrogatory nucleotide of the detection padlock probe, then the control padlock probe but not the detection padlock probe will be circularized and amplified by rolling circle amplification. Thus, mismatch between the nucleotide of interest and the interrogatory interest results in detection of the control padlock barcode only.

In some embodiments, the method comprises contacting a target nucleic acid with one or more control complex and one or more detection complexes to detect one or more alternate regions of interest. In some embodiments, there is provided a first control padlock probe and a first detection complex for detecting a first nucleotide of interest (e.g., a SNP) and a second control padlock probe and a second detection complex for detecting an alternate nucleotide of interest (e.g., an alternate SNP). For example, a first anchor can be designed with an interrogatory region for recognizing the first nucleotide of interest (e.g., C), and a second anchor can be designed with an interrogatory region for recognizing the alternate nucleotide of interest (e.g., A). In some aspects, the methods and compositions provided herein can discriminate between any two nucleotides. For example, the second detection complex can comprise a second control padlock probe comprising a second control padlock barcode, and a second anchor comprising an alternate interrogatory nucleotide (e.g., T) capable of basepairing with the alternate nucleotide of interest (e.g., A) and a second HRc, wherein the second HRc hybridizes to a second HRd of a second control padlock probe comprising a second control padlock barcode. In some cases, circularization of both the second detection padlock probe and the control padlock probe allows for rolling circle amplification of both the control and second detection padlock probes, thereby allowing detection of both the control padlock barcode and the second detection padlock barcode. In some embodiments, the second detection padlock barcode can be the same or different as the first detection padlock barcode. In some embodiments, the second detection padlock barcode is different or can be distinguished from the first detection padlock barcode. In some embodiments, the presence of the first SNP results in detection of the first control padlock barcode and the first detection padlock barcode, while the presence of the alternate SNP results in detection of the second control padlock barcode and the second detection padlock barcode, wherein the second detection padlock probe is the same as or different from the first detection padlock probe. In some embodiments, the barcode of the first control padlock and the barcode of the second control padlock are the same. In some embodiments, the barcode of the first control padlock is different or can be distinguished from the barcode of the second control padlock. In some embodiments, a sample may comprise both variants (e.g., the first nucleotide of interest (e.g., C) and an alternate nucleotide of interest (e.g., A)), and both control padlock barcode, the first detection padlock barcode, and the second detection padlock barcode is detected. In some embodiments, detection of the control barcodes only indicates that neither the SNP detected by the first detection complex nor the alternate SNP detected by the second detection complex is present in the target nucleic acid.

In some embodiments as shown in FIG. 2B, the target nucleic acid comprises from 5′ end to 3′ end: a region HR1′ capable of hybridizing to a region HR1 of the detection padlock probe, a region HR2′ capable of hybridizing to a region HR2 of the anchor, and a region HR3′ capable of hybridizing to a region HR3 of the control padlock. In this 5′ to 3′ configuration, the ligated anchor-splint may function as a primer for rolling circle amplification of the circularized detection padlock probe. In some embodiments, the ligated anchor-splint provides stability for the ligation of the detection padlock probe to form a circular probe and/or to help stabilize the hybridization of the detection padlock/circularized detection padlock probe on the target for in situ amplification. An additional primer can be used to amplify the circularized control padlock probe. In some embodiments, as shown in FIG. 2E, the opposite 5′ to 3′ configuration can be used. Thus, in some embodiments, as shown in FIG. 2E, the target nucleic acid comprises from 3′ end to 5′ end: a region HR1′ capable of hybridizing to a region HR1 of the detection padlock probe, a region HR2′ capable of hybridizing to a region HR2 of the anchor, and a region HR3′ capable of hybridizing to a region HR3 of the control padlock. In this 3′ to 5′ configuration, the anchor can serve as a primer for the circularized control padlock probe by hybridization of HRc to sequence HRd of the control padlock probe, and an additional primer can be used to amplify the circularized detection padlock probe. In some embodiments, the ligated anchor-splint provides stability for the ligation of the detection padlock probe to form a circular probe and/or to help stabilize the hybridization of the detection padlock/circularized.

In some embodiments, as shown in of FIGS. 2C and 2F, the detection padlock probe comprises an interrogatory sequence within HR1 at one end of the detection padlock probe (e.g., an interrogatory region at the 5′ end or 3′ end of the detection padlock probe, as shown in FIG. 2C and FIG. 2F, respectively) that hybridizes to a region of interest at one end of HR1′ (e.g., at the 3′ end or 5′ end of HR1′, as shown in FIG. 2C and FIG. 2F, respectively). As shown FIG. 2C, hybridization of a region HRb of the anchor to HRa of the detection padlock probe (e.g., the interrogatory region and HRa are at the 5′ and 3′ ends of the detection padlock probe, respectively as shown in FIG. 2C, or vice versa, as shown in FIG. 2F) can allow the anchor to serve as a template to circularize the detection padlock probe. In some embodiments, the HRb of anchor hybridizing to HRa of the detection padlock probe brings the ends of the padlock probe in sufficient proximity for ligation. In some embodiments, the control padlock probe comprises hybridization regions HR3 and HRd, the padlock probe comprises hybridization regions HR1 and HRa, the anchor comprises hybridization regions HR2, HRb, and HRc, the target nucleic acid comprises adjacent hybridization regions HR1′, HR2′, and HR3′, wherein HR1′ comprises a region of interest at a terminus of HR1′, HR1 hybridizes to HR1′, wherein HR1 comprises a nucleotide complementary to the region of interest in HR1′ and the complementary nucleotide is at a terminus of the detection padlock probe, HR2 hybridizes to HR2′, HR3 hybridizes to HR3′, HRa hybridizes to HRb, and HRc hybridizes to HRd.

In FIGS. 2A, 2C, 2D, and FIG. 2F, the detection padlock comprises the interrogatory region. Since a detection padlock barcode sequence is physically coupled to the interrogatory region (e.g., comprising a nucleotide complementary to a single nucleotide of interest), the detection padlock barcode may correspond to or be associated with the interrogatory region and the region of interest in the target nucleic acid. In some embodiments, the detection padlock comprises a detection padlock barcode sequence that corresponds to a sequence in the anchor, for example, a sequence that is used for splint ligation of the control padlock probe. In some examples, the detection padlock barcode sequence corresponds to a sequence of HRc and/or HRd. In some embodiments, the control padlock comprises a control padlock barcode sequence that corresponds to a sequence in the target nucleic acid (e.g., a sequence in a region of interest) and/or a sequence in the anchor (e.g., a sequence that is used for splint ligation of the control padlock probe). In some examples, the control padlock barcode sequence corresponds to a sequence of HRc and/or HRd. In some examples, as shown in FIGS. 2B and 2E, the detection padlock barcode and the interrogatory region (which is in the anchor) are not physically coupled. However, the control padlock (comprising a control padlock barcode) and the anchor (comprising the interrogatory region) are physically coupled through HRc/HRd hybridization. Thus, a control padlock barcode sequence may be designed to correspond to HRc/HRd, which in turn corresponds to the interrogatory region and the region of interest in the target nucleic acid.

In some embodiments, a plurality of control padlock probes are provided, and each padlock probe can comprise a control barcode that is uniquely associated with a detection padlock probe (e.g., associated with a region of interest). In some cases, the plurality of control padlock probes each have a different barcode sequence from other control padlock probes. In some embodiments, the plurality of padlock probes shares at least some common sequences in the padlock probe. For example, a plurality of control padlock probes may have the same sequence at HRd. In some cases, a plurality of control padlock probes may have the different sequence at HRd, each associated with a particular detection padlock probe.

FIGS. 2C and 2F show exemplary embodiments of a control complex and a detection complex wherein the padlock probe comprises an interrogatory region at a terminus of HR1 that hybridizes to a region of interest at a terminus of HR1′, and the anchor comprises a hybridization region HRc that hybridizes to HRd. In some embodiments, the interrogatory region is a single interrogatory nucleotide capable of basepairing with a single nucleotide of interest (e.g., a SNP). In some embodiments as shown in FIG. 2C, the control probe comprises hybridization regions HR3 and HRd, the padlock probe comprises hybridization regions HR1 and HRa, the anchor comprises hybridization region HR2, HRb, and HRc, and the target nucleic acid comprises adjacent hybridization regions HR1′, HR2′, and HR3′, wherein the 3′ end nucleotide of HR1′ is a single nucleotide of interest, HR1 hybridizes to HR1′, wherein the 5′ end nucleotide of HR1 is the 5′ end nucleotide of the padlock probe and is complementary to the single nucleotide of interest in HR1′, HR2 hybridizes to HR2′, wherein the 3′ end nucleotide of HR2 is complementary to the 5′ end nucleotide of HR2′, and HRa hybridizes to HRb, wherein the 3′ end nucleotide of HRa is the 3′ end nucleotide of the padlock probe and is complementary to the nucleotide immediately adjacent to the 3′ end nucleotide of HR2. When there is a match between the interrogatory nucleotide and the nucleotide of interest in the target nucleic acid, the interrogatory nucleotide is forced to be in proximity of the 5′ end of the detection padlock by hybridization of the anchor HR2 and HRb regions to HR2′ and HRa, respectively, allowing ligation of the detection padlock probe. In some embodiments, the HRb of anchor hybridizing to HRa of the detection padlock probe brings the ends of the padlock probe in sufficient proximity for ligation.

In some embodiments, the method comprises contacting a target nucleic acid with a control complex and one or more detection complexes. In some embodiments, there is provided a first detection complex for detecting a first nucleotide of interest (e.g., a SNP) and a second detection complex for detecting an alternate nucleotide of interest (e.g., an alternate SNP). In an exemplary embodiment, the first nucleotide of interest is C, and the alternate nucleotide of interest is A. However, the methods and compositions provided herein can discriminate between any two nucleotides. In some case, the second detection complex can comprise a second detection padlock barcode and an alternate interrogatory nucleotide (e.g., T) capable of basepairing with an alternate nucleotide of interest (e.g., A). Circularization of both the second detection padlock probe and the control padlock probe allows for rolling circle amplification of both the control and second detection padlock probes, thereby allowing detection of both the control padlock barcode and the second detection padlock barcode. In some embodiments, the presence of the first SNP results in detection of the control padlock barcode and the first detection padlock barcode, while the presence of the alternate SNP results in detection of the control padlock barcode and the second detection padlock barcode. In some embodiments, a sample may comprise both variants (e.g., the first nucleotide of interest (e.g., C) and an alternate nucleotide of interest (e.g., A)), such the control padlock barcode(s), the first detection padlock barcode, and the second detection padlock barcode is detected. In some embodiments, detection of the control barcode only indicates that neither the SNP detected by the first detection padlock probe nor the alternate SNP detected by the second detection padlock probe is present in the target nucleic acid.

In some embodiments as shown in FIG. 2F, the control probe comprises hybridization regions HR3 and HRd, the padlock probe comprises hybridization regions HR1 and HRa, the anchor comprises hybridization region HR2, HRb, and HRc, the target nucleic acid comprises adjacent hybridization regions HR1′, HR2′, and HR3′, wherein the 5′ end nucleotide of HR1′ is a single nucleotide of interest, HR1 hybridizes to HR1′, wherein the 3′ end nucleotide of HR1 is the 3′ end nucleotide of the padlock probe and is complementary to the single nucleotide of interest in HR1′, HR2 hybridizes to HR2′, HR3 hybridizes to HR3′, and HR wherein the 5′ end nucleotide of HR2 is complementary to the 3′ end nucleotide of HR2′, and HRa hybridizes to HRb, wherein the 5′ end nucleotide of HRa is the 5′ end nucleotide of the padlock probe and is complementary to the nucleotide immediately adjacent to the 5′ end nucleotide of HR2. When there is a match between the interrogatory nucleotide and the nucleotide of interest in the target nucleic acid, the interrogatory nucleotide is forced to be in proximity of the 5′ end of the detection padlock by hybridization of the anchor HR2 and HRb regions to HR2′ and HRa, respectively, allowing ligation of the detection padlock probe.

In some aspects, one or more of the polynucleotides comprise one or more barcodes. In some instances, the detection padlock probe comprises from 5′ to 3′: HRa-barcode(s)-HR1. In another example, the detection padlock probe comprises from 5′ to 3′: HR1-barcode(s)-HRa. In some instances, the control padlock probe comprises from 5′ to 3′: HRd-barcode(s)-HR3. In another example, the control padlock probe comprises from 5′ to 3′: HR3-barcode(s)-HRd. In some aspects, one or more barcodes are comprised in the amplified polynucleotide (e.g., present in the control and detection padlock probes). In some embodiments, a detection padlock probe may comprise two or more barcodes. In some embodiments, one or more barcode are on a polynucleotide that is not amplified (e.g., the anchor) and can provide a platform for targeting functionalities, such as oligonucleotides, oligonucleotide-antibody conjugates, oligonucleotide-streptavidin conjugates, modified oligonucleotides, affinity purification, detectable moieties, enzymes, enzymes for detection assays or other functionalities, and/or for detection and identification of the region of interest. In some of any such embodiments, the barcode of a control padlock associated with a detection complex is different from a barcode of a control padlock associated with a different detection complex. In some embodiments, the detection padlock barcode associated with each region of interest (e.g., single nucleotide of interest) is different or can be distinguished from the barcodes associated with other region of interests.

In some embodiments, one or more of the barcodes disclosed herein can be correlated with the sequence complementary to the analyte (e.g., an interrogatory region), and thus a particular analyte (e.g., a region of interest). A number (n) of analytes (e.g., regions of interest or single nucleotides of interest) can be examined by introducing (n) different sequences complementary to an analyte/barcode pluralities to the sample. In some embodiments, sequences complementary to an analyte (e.g., regions of interest) can be used in multiplexed methods to analyze 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, or more analytes (e.g., regions of interest). In some embodiments, the regions of interest can be from the same target nucleic acid or different target nucleic acids.

In some embodiments, methods provided herein comprise analyzing the one or more barcodes of the polynucleotides using multiplexed in situ imaging. In some embodiments, analyzing the barcode comprises employing exemplary barcode detection schemes such as described in RNA sequential probing of targets (RNA SPOTs), sequential fluorescent in situ hybridization (seqFISH), single-molecule fluorescent in situ hybridization (smFISH), multiplexed error-robust fluorescence in situ hybridization (MERFISH), in situ sequencing, hybridization-based in situ sequencing (HybISS), targeted in situ sequencing, fluorescent in situ sequencing (FISSEQ), or spatially-resolved transcript amplicon readout mapping (STARmap). In some embodiments, the methods provided herein comprise analyzing the barcode of the composite padlock or circular probes by sequential hybridization and detection with a plurality of probes (e.g., labelled probes). In some embodiments, detection comprises sequential hybridization of oligonucleotide probes to the barcode sequence or a complementary sequence (e.g., of the ligated padlock probe, or a complementary sequence in the amplification product generated using the padlock probe as a template). In some embodiments, labelled probes can be indirectly or directly hybridized to the amplification product generated using the padlock probe as a template. In some aspects, sequential hybridization may comprise hybridizing labelled probes to oligonucleotide probes (e.g., secondary probes) hybridized to the amplification product generated using the padlock probe as a template. A variety of light-based sequencing technologies are known in the art. See, e.g., Landegren et al., Genome Res. 8:769-76 (1998); Kwok, Pharmocogenomics 1:95-100 (2000); and Shi, Clin. Chem. 47:164-172 (2001), which are herein incorporated by reference in their entirety. Various probe designs may be paired with a control padlock probe and detected as provided herein, for example, a probe or probe set capable of RNA-templated ligation can be used (e.g., U.S. Pat. Pub. 2020/0224244 which is hereby incorporated by reference in its entirety).

In some aspects, the barcode provides information for identification of the target nucleic acid and/or one or more regions of interest contained therein. In some aspects, one or more barcode is used to convey or is capable of conveying information (e.g., information about a target nucleic acid in a sample or a molecule such as a polynucleotide), such as a nucleic acid sequence that is used to identify, e.g., a single cell or a subpopulation of cells or a single target nucleic acid or a subset of target nucleic acids. Barcodes can be linked to a target nucleic acid of interest or region of interest during amplification and used to trace back the amplicon to, for example, the cell and/or position in a tissue from which the target nucleic acid originated. A barcode can be added to a target nucleic acid of interest during amplification by carrying out amplification with a polynucleotide that contains a region comprising the barcode and a region that is complementary to the target nucleic acid such that the barcode is incorporated into the final amplified target nucleic acid product (i.e., amplicon). A particular barcode can be unique relative to other barcodes. A barcode can be attached to an analyte or to another moiety or structure in a reversible or irreversible manner. A barcode can be added to, for example, a fragment of a deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) sample before or during sequencing of the sample. Barcodes can allow for identification and/or quantification of individual sequencing-reads (e.g., a barcode can be or can comprise a unique molecular identifier or “UMI”).

In some embodiments, the barcode sequences comprise 4′ complexity given a sequencing read of N bases, and a much shorter sequencing read may be required for molecular identification by non-barcode sequencing methods such as direct sequencing of an RNA target or a cDNA. For example, 1024 molecular species may be identified using a 5-nucleotide barcode sequence (4⁵=1024), whereas 8 nucleotide barcodes can be used to identify up to 65,536 molecular species, a number greater than the total number of distinct genes in the human genome. In some embodiments, the barcode sequences contained in the probes are detected, rather than endogenous sequences, which can be an efficient read-out in terms of information per cycle of sequencing. Because the barcode sequences are pre-determined, they can also be designed to feature error detection and correction mechanisms.

In some aspects, one or more of the polynucleotides comprise one or more barcodes. In some aspects, at least two, three, four, five, six, seven, eight, nine, 10, or more barcodes are comprised in the padlock or circular probe formed of the plurality of polynucleotides.

Barcodes can spatially-resolve molecular components found in biological samples, for example, within a cell or a tissue sample. In some embodiments, a barcode comprises two or more sub-barcodes or partial barcodes that together function as a single barcode. For example, a polynucleotide barcode can comprise two or more polynucleotide sequences (e.g., sub-barcodes) that are separated by one or more non-barcode sequences.

In some embodiments, the provided polynucleotides comprise one or more primary barcode sequences. In some embodiments, an amplification product (e.g., an RCA product) comprises multiple copies of the one or more primary barcode sequences or complementary sequences thereof, and the amplification product is detected using one or more detection probes (e.g., a detectably labeled oligo such as fluorescent oligonucleotides) that hybridize to the one or more primary barcode sequences or complementary sequences thereof.

In some embodiments, the method further comprises using one or more secondary probes that hybridize to the one or more primary barcode sequences or complementary sequences thereof on one or more primary probes that bind to a target nucleic acid such as an mRNA. In some embodiments, the one or more secondary probes hybridize to an amplification product (e.g., an RCA product) comprising multiple copies of the one or more primary barcode sequences or complementary sequences thereof. In some embodiments, the one or more secondary probes comprise one or more secondary barcode sequences and are detected using one or more detection probes (e.g., a detectably labeled oligo such as fluorescent oligonucleotides) that hybridize to the one or more second barcode sequences or complementary sequences thereof.

In any of the embodiments, barcodes (e.g., primary and/or secondary barcode sequences) can be analyzed (e.g., detected or sequenced) using any suitable methods or techniques, comprising those described herein, for example, in Section VI.

In some aspects, the methods provided herein comprise the use of a polynucleotide that is an anchor. In some aspects, the anchor contains an HR (also referred to as HR2 herein) that hybridizes to an HR (also referred to as HR2′ herein) contained in the target nucleic acid. In FIGS. 2A-2F, the HR of the anchor is labelled as HR2 and hybridizes to the HR of the target nucleic acid labelled HR2′. In some embodiments, the HR of the anchor comprises an interrogatory region complementary to the region of interest contained in the target nucleic acid. In some embodiments, the complementary interrogatory region hybridizes to the region of interest. In certain aspects, the anchor is designed such that ligation and/or amplification of one or more polynucleotides is dependent on hybridization of the anchor to the target nucleic acid and an interrogatory region in the anchor basepairing to the region of interest.

In some embodiments, the complementary interrogatory region is at or near the 3′ end of the HR, as is depicted in FIG. 2B. In other embodiments, the complementary interrogatory region is at or near the 5′ end of the HR, as is depicted in FIG. 2E.

In some embodiments, the complementary interrogatory region comprises one of the five terminal nucleotide positions of the HR (e.g., HR1 or HR2), either on the 3′ or on the 5′ end. In some embodiments, the complementary interrogatory region comprises the fifth terminal nucleotide position of the HR. In some embodiments, the complementary interrogatory region comprises the fourth terminal nucleotide position of the HR. In some embodiments, the complementary interrogatory region comprises the third terminal nucleotide position of the HR. In some embodiments, the complementary interrogatory region comprises the second terminal nucleotide position of the HR. In some embodiments, the complementary interrogatory region comprises the terminus of the HR. In some embodiments, the complementary interrogatory region comprises the 5′ end nucleotide of the HR. In other embodiments, the complementary interrogatory region comprises the 3′ end nucleotide of the HR. In some embodiments, the complementary interrogatory region of the anchor for hybridizing with the region of interest comprises one of the five terminal nucleotide positions of the anchor oligo. In some embodiments, the complementary interrogatory region of the anchor for hybridizing with the region of interest comprises the 5′ end nucleotide of the anchor oligo. In other embodiments, the complementary interrogatory region of the anchor for hybridizing with the region of interest comprises the 3′ end nucleotide of the anchor oligo. In some embodiments, the complementary interrogatory region of the anchor is a single nucleotide, such as any one of the nucleotides described above.

In some embodiments, the complementary interrogatory region is the 3′ end nucleotide of the anchor HR, and the single nucleotide of interest is the 5′ end nucleotide of the target nucleic acid HR that hybridizes to the anchor HR, as is depicted in FIG. 2B. In other embodiments, the complementary interrogatory region is the 5′ end nucleotide of the anchor HR, and the single nucleotide of interest is the 3′ end nucleotide of the target nucleic acid HR that hybridizes to the anchor HR, as is depicted in FIG. 2E.

The anchor may be of any suitable length. For example, the anchor selected may depend on characteristics of the hybridization of the anchor to the target nucleic acid (e.g., stability of the anchor to the target nucleic acid in the presence or absence of a matching or mismatched region of interest in the target nucleic acid). In some embodiments, the anchor is between or between about 5 and 200 nucleotides in length. In some embodiments, the anchor is between or between about 5 and 100 nucleotides in length. In some embodiments, the anchor is between or between about 15 and 200 nucleotides in length. In some embodiments, the anchor is between or between about 5 and 40 nucleotides in length. In some embodiments, the anchor is between or between about 5 and 15 nucleotides in length. In some embodiments, the anchor is between or between about 15 and 20 nucleotides in length. In some embodiments, the anchor is between or between about 20 and 25 nucleotides in length. In some embodiments, the anchor is between or between about 25 and 30 nucleotides in length. In some embodiments, the anchor is between or between about 30 and 35 nucleotides in length. In some embodiments, the anchor is between or between about 25 and 30 nucleotides in length. In some embodiments, the anchor is between or between about 35 and 40 nucleotides in length.

In some embodiments, the anchor HR (also referred to as HR2 herein) is between or between about 5 and 200 nucleotides in length. In some embodiments, the anchor HR is between or between about 5 and 100 nucleotides in length. In some embodiments, the anchor HR is between or between about 15 and 200 nucleotides in length. In some embodiments, the anchor HR is between or between about 5 and 40 nucleotides in length. In some embodiments, the anchor HR is between or between about 5 and 15 nucleotides in length. In some embodiments, the anchor HR is between or between about 15 and 20 nucleotides in length. In some embodiments, the anchor HR is between or between about 20 and 25 nucleotides in length. In some embodiments, the anchor HR is between or between about 25 and 30 nucleotides in length. In some embodiments, the anchor HR is between or between about 30 and 35 nucleotides in length. In some embodiments, the anchor HR is between or between about 25 and 30 nucleotides in length. In some embodiments, the anchor HR is between or between about 35 and 40 nucleotides in length.

In some embodiments, HR2, HR1, and HR3 are independently between or between about 5 and 200 nucleotides in length. In some embodiments, HR2, HR1, and HR3 are independently between or between about 15 and 200 nucleotides in length. In some embodiments, HR2, HR1, and HR3 are independently between or between about 15 and 100 nucleotides in length. In some embodiment, HR2, HR1, and HR3 are independently at least 10, 15, or 20 nucleotides in length.

In some embodiments, HRc has a melting temperature for hybridization to HRd that is lower than the melting temperature of the anchor for hybridization to the target nucleic acid (e.g., the melting temperature of HRc and HRd is lower than the melting temperature of HR2 and HR2′). In some embodiments, HRc is shorter than HR2. In some embodiments, HRc is between or between about 3 and 50, 3 and 40, 3 and 30, 3 and 20, 3 and 15, or 3 and 10 nucleotides in length. In some embodiments, HRc is between or between about 3 and 10 nucleotides in length. In some embodiments, HRc is no more than 5, 10, 15, or 20 nucleotides in length.

In some embodiments, HRb has a melting temperature for hybridization to HRa that is lower than the melting temperature of the anchor for hybridization to the target nucleic acid (e.g., the melting temperature of HRb and HRa is lower than the melting temperature of HR2 and HR2′). In some embodiments, HRb is shorter than HR2. In some embodiments, HRb is between or between about 3 and 50, 3 and 40, 3 and 30, 3 and 20, 3 and 15, or 3 and 10 nucleotides in length. In some embodiments, HRb is between or between about 3 and 10 nucleotides in length. In some embodiments, HRb is no more than 5, 10, 15, or 20 nucleotides in length.

In some embodiments, the anchor also comprises an overhang sequence, e.g., a sequence that does not hybridize to the target nucleic acid or to other polynucleotides. In certain aspects, the overhang provides a sequence by which the anchor can be detected, for instance via in situ hybridization to the overhang sequence. In some embodiments, the overhang comprises a primer binding region. In some cases, the anchor can be detected by binding a primer to a sequence comprised by the anchor and generating an extension product for detection. In some embodiments, the overhang comprises a barcode sequence, optionally one or more additional barcode sequences. In some embodiments, the barcode sequence identifies a nucleic acid sequence and/or the region of interest. In some embodiments, the overhang is between or between about 5 and 40 nucleotides in length. In some embodiments, the overhang is between or between about 5 and 15 nucleotides in length. In some embodiments, the overhang is between or between about 15 and 20 nucleotides in length. In some embodiments, the overhang is between or between about 20 and 25 nucleotides in length. In some embodiments, the overhang is between or between about 25 and 30 nucleotides in length. In some embodiments, the overhang is between or between about 30 and 35 nucleotides in length. In some embodiments, the overhang is between or between about 25 and 30 nucleotides in length. In some embodiments, the overhang is between or between about 35 and 40 nucleotides in length.

In some embodiments, the anchor is a DNA molecule. In some embodiments, the anchor is an RNA molecule or comprises ribonucleotides. In some embodiments, the anchor comprises a HR that is a DNA binding region. In some embodiments, the anchor comprises a HR that is an RNA binding region. In some embodiments, the anchor is a modified nucleic acid molecule or contains modified nucleotides or modified nucleosides, such as methylated nucleotides and nucleotide analogs, uracyl, other sugars, and linking groups such as fluororibose and thioate, and nucleotide branches. In some embodiments, the anchor may comprise non-nucleotide components. In some embodiments, the anchor may be modified to comprise N3′-P5′ (NP) phosphoramidate, morpholino phosphorociamidate (MF), locked nucleic acid (LNA), 2′-O-methoxyethyl (MOE), or 2′-fluoro, arabino-nucleic acid (FANA). In some embodiments, the anchor comprises LNA. In some aspects, the modification can be used as an anchoring site to anchor the amplified product to the scaffold, other probe polynucleotides, other amplification products and/or to cellular structures, e.g., as described in Section V below.

In some aspects, the methods provided herein also comprise the use of two or more polynucleotides that are padlock probes. In some embodiments, the two or more polynucleotides that are padlock probes comprise a detection padlock probe and a control padlock probe.

In some aspects, the detection padlock probe and the control padlock probe each comprise at least two HRs. In FIGS. 2A-2F, two HRs of the detection padlock probe are labelled as HRa and HR1 and two HRs of the control padlock probe are labelled as HR3 and HRd. In some embodiments, the HRs of a padlock probe are adjacent to one another. In some embodiments, the HRs of a padlock probe are immediately adjacent to one another. In other embodiments, the HRs of a padlock probe are not immediately adjacent to one another and instead are separated by a gap, for instance a gap of 1, 2, 3, 4, or 5 nucleotides. In some embodiments, the HRs of a padlock probe are not immediately adjacent to one another and instead are separated a barcode sequence. In some aspects, one of the HRs contained in the padlock probe hybridizes to an HR in the target nucleic acid that does not contain the region of interest. For instance, in FIGS. 2B and 2E, the HR labelled as HR1 of the padlock probe hybridizes to the HR of the target nucleic acid labelled as HR1′.

In some embodiments, HR1 of the detection padlock probe is between or between about 5 and 40 nucleotides in length. In some embodiments, HR1 is between or between about 5 and 15 nucleotides in length. In some embodiments, HR1 is between or between about 15 and 20 nucleotides in length. In some embodiments, HR1 is between or between about 20 and 25 nucleotides in length. In some embodiments, HR1 is between or between about 25 and 30 nucleotides in length. In some embodiments, HR1 is between or between about 30 and 35 nucleotides in length. In some embodiments, HR1 is between or between about 25 and 30 nucleotides in length. In some embodiments, HR1 is between or between about 35 and 40 nucleotides in length.

In some embodiments, HR3 of the control padlock probe is between or between about 5 and 40 nucleotides in length. In some embodiments, HR3 is between or between about 5 and 15 nucleotides in length. In some embodiments, HR3 is between or between about 15 and 20 nucleotides in length. In some embodiments, HR3 is between or between about 20 and 25 nucleotides in length. In some embodiments, HR3 is between or between about 25 and 30 nucleotides in length. In some embodiments, HR3 is between or between about 30 and 35 nucleotides in length. In some embodiments, HR3 is between or between about 25 and 30 nucleotides in length. In some embodiments, HR3 is between or between about 35 and 40 nucleotides in length.

In some embodiments, the length of HR1 of the detection padlock, HR3 of the control padlock, and/or HR2 of the anchor is about 4 to about 40 nucleotides, such as about 4 to about 16 nucleotides or about 8 to about 12 nucleotides. In some aspects, the length of HR1 of the detection padlock, HR3 of the control padlock, and/or HR2 of the anchor is fewer than 20 nucleotides, such as 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 nucleotides. In some embodiments, for example, as shown in FIGS. 4A-4D, ligation of the anchor to a splint stabilizes hybridization of the detection and/or control padlock probes (or the circularized probe) to the target, thereby allowing the primer to hybridize to the circularized detection or control probe and initiate rolling circle amplification.

In some embodiments, the T_(m) of the padlock/target hybridization and the T_(m) of the anchor/target hybridization are the same or substantially the same, e.g., differing by less than 0.1° C., less than 0.5° C., less than 1° C., less than 2° C., less than 3° C., less than 4° C., or less than 5° C. In some embodiments, the length of HR1 of the detection padlock, HR3 of the control padlock, and/or HR2 of the anchor are the same or substantially the same, e.g., differing by 1, 2 or fewer, 3 or fewer, 4 or fewer, 5 or fewer, 6 or fewer, 7 or fewer, 8 or fewer, 9 or fewer, or 10 or fewer nucleotides.

In some embodiments, the T_(m) of the padlock/target hybridization and/or the T_(m) of the anchor/target hybridization are between or between about 40° C. and about 70° C., such as between at or about 50° C. and at or about 65° C., or at or about 60° C.

In some aspects, the methods provided herein also comprise the use of a polynucleotide that is a splint. In some aspects, the detection padlock probe and the splint for use in the methods provided herein hybridize to one another such that the detection padlock probe is circularized, e.g., for instance to be used as a template for amplification. Thus, in some aspects, the detection padlock probe and splint are designed such that subsequent ligation and/or amplification of one of the polynucleotides is dependent on hybridization of the detection padlock probe and the split to one another, as well as hybridization of the detection padlock probe to the target nucleic acid. In some aspects, the methods provided herein use a circular probe.

In some embodiments, the T_(m) of the splint/padlock hybridization is lower than the T_(m) of the detection padlock/target hybridization and/or the T_(m) of the anchor/target hybridization, e.g., by at least or at least about 5° C., at least or at least about 10° C., at least or at least about 15° C., at least or at least about 20° C., at least or at least about 25° C., at least or at least about 30° C., at least or at least about 40° C., at least or at least about 45° C., or at least or at least about 50° C. lower. In some embodiments, the T_(m) of the splint/padlock hybridization is between or between about 15° C. and about 50° C., such as between at or about 20° C. and at or about 40° C., between at or about 25° C. and at or about 30° C., or at or about 27° C.

In some aspects, the detection and/or padlock probe contains another HR that is a split region, i.e. containing sequences at both ends of the padlock probe, also referred to as HRa in the detection padlock probe or HRd in the control padlock probe herein. In some aspects, the HR that is a split region hybridizes to an HR contained in a splint, also referred to as HRb herein. In some aspects, a “splint” or a “splint template” is a nucleotide sequence that, when hybridized to other nucleotide sequences, acts as a “splint” to position the polynucleotides next to one another so that they can be ligated together. In some embodiments, the splint assists in ligating a 5′ end and a 3′ end of a linear polynucleotide, for instance the detection padlock probe, resulting in a circular polynucleotide. In FIGS. 2B and 2E, the split HR of the detection padlock probe is labelled HRa and hybridizes to the HR contained in the splint labelled HRb. In some embodiments, the 5′ and 3′ end nucleotides of the detection padlock probe hybridize to the splint. In some embodiments, the 5′ and 3′ end nucleotides of the detection padlock probe that are hybridized to the splint are directly adjacent to one another. In other embodiments, the 5′ and 3′ end nucleotides of the detection padlock probe that are hybridized to the splint are not directly adjacent to one another and instead are separated by a gap, for instance a gap of 1, 2, 3, 4, or 5 nucleotides.

In some embodiments, the HR that is a split region of the control probe hybridizes to an HR contained in the anchor probe, also referred to as HRc herein. In some embodiments, the region HRc of the anchor assists in ligating a 5′ end and a 3′ end of a linear polynucleotide, for instance the control padlock probe, resulting in a circular polynucleotide. In FIGS. 2A-2F, the split HR of the control padlock probe is labelled HRd and hybridizes to the HR contained in the anchor labelled HRa. In some embodiments, the 5′ and 3′ end nucleotides of the control padlock probe hybridize to the anchor. In some embodiments, the 5′ and 3′ end nucleotides of the control padlock probe that are hybridized to the anchor are directly adjacent to one another. In other embodiments, the 5′ and 3′ end nucleotides of the control padlock probe that are hybridized to the anchor are not directly adjacent to one another and instead are separated by a gap, for instance a gap of 1, 2, 3, 4, or 5 nucleotides.

In some embodiments, the split HR of the padlock probe is between or between about 5 and about 20 nucleotides in length. In some embodiments, the split HR of the padlock probe is between or between about 5 and about 15 nucleotides in length. In some embodiments, the split HR of the padlock probe is between or between about 5 and about 10 nucleotides in length.

The splint may be of any suitable length. For example, the splint selected may depend on characteristics of the hybridization of the splint to the padlock probe (e.g., stability). In some embodiments, the splint is between or between about 5 and about 20 nucleotides in length. In some embodiments, the splint is between or between about 5 and about 15 nucleotides in length. In some embodiments, the splint is between or between about 5 and about 10 nucleotides in length. In some embodiments, the splint is shorter than about 5 nucleotides in length.

In some embodiments, the splint HR (e.g., HRb) is between or between about 5 and about 20 nucleotides in length. In some embodiments, the splint HR is between or between about 5 and about 15 nucleotides in length. In some embodiments, the splint HR is between or between about 5 and about 10 nucleotides in length. In some embodiments, the splint HR is shorter than about 5 nucleotides in length.

In some embodiments, the length of the splint is selected such that the ability of splint HRb to facilitate ligation of the padlock probe ends to form a circular probe is dependent (e.g., conditional) on the ligation to form the anchor-splint. In some examples, the splint or splint HR is sufficiently short such that ligation of the padlock probe to form a circular probe may not occur without ligation to form the anchor-splint.

In some embodiments, the 5′ end nucleotide of the splint and/or the splint HR is complementary to the 3′ end nucleotide of the split HR of the padlock probe. In some embodiments, the 5′ end nucleotide of the splint and/or the splint HR hybridizes to the 3′ end nucleotide of the split HR of the detection padlock probe. In other embodiments, the 3′ end nucleotide of the splint and/or the splint HR is complementary to the 5′ end nucleotide of the split HR of the detection padlock probe. In some embodiments, the 3′ end nucleotide of the splint and/or the splint HR hybridizes to the 5′ end nucleotide of the split HR of the detection padlock probe.

In some aspects, any of the padlock probes described herein can further comprise a sequence by which the padlock probe or amplification product thereof can be analyzed, e.g., detected or sequenced. In some embodiments, the HRa or HRd of the detection or control padlock probe contains a sequence by which the region of interest can be identified. In some embodiments, a sequence by which the region of interest can be identified is 2, 4, 6, 8, or 10 nucleotides in length.

In some aspects, any one of the padlock probes described herein can comprise a barcode sequence that identifies a nucleic acid sequence or a region of interest, for instance the region of interest contained in the target nucleic acid. In some embodiments, the padlock probe contains one or more other barcode sequences. In some embodiments, one of the one or more other barcode sequences identifies the target nucleic acid, e.g., an mRNA, as a splice variant. In some embodiments, one of the one or more other barcode sequences identifies a splice junction sequence.

In some embodiments, the barcode sequences and/or the one or more other barcode sequences are between the HRa or HRd of the detection or control padlock probe that hybridizes to the anchor and/or splint and the HR of the detection or control padlock probe that hybridizes to the target nucleic acid. In some embodiments, the barcode sequences and/or the one or more other barcode sequences are between the 3′ end of the HR of the padlock probe that hybridizes to the target nucleic acid and the 5′ end of HRa or HRd in the detection or control probes, respectively. In other embodiments, the barcode sequences and/or the one or more other barcode sequences are between the 5′ end of the HR of the padlock probe that hybridizes to the target nucleic acid and the 3′ end of HRa or HRd in the detection or control probes, respectively.

In some aspects, one or more barcode sequences of a detection padlock probe are flanked by HRa on one side and HR1 (or portion thereof) on the other side in the detection padlock probe polynucleotide. For example, the padlock probe comprises from 5′ to 3′: HRa-barcode(s)-HR1. In another example, the padlock probe comprises from 5′ to 3′: HR1-barcode(s)-HRa. In some embodiments, HR1 is a split region and the detection padlock probe comprises: a first portion of the split region HR1-HRa-barcode(s)-a second portion of the split region HR1.

In some aspects, one or more barcode sequences of a control padlock probe are flanked by HRd (or portion thereof) on one side and HR3 on the other side in the control padlock probe polynucleotide. For example, the padlock probe comprises from 5′ to 3′: HRd-barcode(s)-HR3. In another example, the padlock probe comprises from 5′ to 3′: HR3-barcode(s)-HRd. In some embodiments, HRd is a split region and the detection padlock probe comprises: a first portion of the split region HRd-HR3-barcode(s)-a second portion of the split region HRd.

In some embodiments, the 3′ end nucleotide of the anchor HR is juxtaposed to, but does not basepair with the 5′ end nucleotide of HR1 (the HR of the detection padlock probe that hybridizes to the target nucleic acid), as is depicted in FIG. 2B. In other embodiments, the 5′ end nucleotide of the anchor HR is juxtaposed to, but does not basepair with the 3′ end nucleotide of HR1 (the HR of the padlock probe that hybridizes to the target nucleic acid), as is depicted in FIG. 2E. In some embodiments, the anchor and the splint are included in formation of a tri-fork hybridization complex and the anchor and the splint are ligated in the tri-fork hybridization complex, e.g., as described in Section IV.

In some embodiments, the splint is a DNA molecule. In some embodiments, the splint is an RNA molecule or comprises ribonucleotides. In some embodiments, the splint is a modified nucleic acid molecule or contains modified nucleotides or modified nucleosides, such as methylated nucleotides and nucleotide analogs, uracyl, other sugars, and linking groups such as fluororibose and thioate, and nucleotide branches. In some embodiments, the splint may comprise non-nucleotide components. In some embodiments, the splint may be modified to comprise N3′-P5′ (NP) phosphoramidate, morpholino phosphorociamidate (MF), locked nucleic acid (LNA), 2′-O-methoxyethyl (MOE), or 2′-fluoro, arabino-nucleic acid (FANA). In some embodiments, the splint comprises LNA. In some aspects, the modification can be used as an anchoring site to anchor the amplified product to the scaffold, other probe polynucleotides, other amplification products and/or to cellular structures, e.g., as described in Section V below.

In some embodiments, any one of the padlock probes described herein is a DNA molecule. In some embodiments, the padlock probe is an RNA molecule or comprises ribonucleotides. In some embodiments, the padlock probe is a modified nucleic acid molecule or contains modified nucleotides or modified nucleosides, such as methylated nucleotides and nucleotide analogs, uracyl, other sugars, and linking groups such as fluororibose and thioate, and nucleotide branches. In some embodiments, the padlock probe may comprise non-nucleotide components. In some embodiments, the padlock probe may be modified to comprise N3′-P5′ (NP) phosphoramidate, morpholino phosphorociamidate (MF), locked nucleic acid (LNA), 2′-O-methoxyethyl (MOE), or 2′-fluoro, arabino-nucleic acid (FANA). In some embodiments, the padlock probe comprises LNA. In some aspects, the modification can be used as an anchoring site to anchor the amplified product to the scaffold, other probe polynucleotides, other amplification products and/or to cellular structures, e.g., as described in Section V below.

In some embodiments, the polynucleotides provided herein further comprise one or more additional primers for amplifying the control and/or detection padlock probes. In some embodiments, the primer comprises a sequence that can hybridizes any portion of the detection padlock probe or the control padlock probe. In some embodiments, the primer comprises a sequence that hybridizes to the target nucleic acid, in addition to a sequence that hybridizes to the circularized detection padlock probe or the circularized control padlock probe. In some embodiments, the anchor is used as a primer for rolling circle amplification of the circularized detection padlock probe or the circularized control padlock probe. In some embodiments, the anchor is used as a primer for rolling circle amplification of the circularized detection padlock probe and a separate primer is provided for rolling circle amplification of the circularized control padlock probe. In some embodiments, the anchor is used as a primer for rolling circle amplification of the circularized control padlock probe and a separate primer is provided for rolling circle amplification of the circularized detection padlock probe. The primer can comprise a region for hybridization to the padlock probe. Optionally, the primer can comprise a region for hybridization to a region of the target nucleic acid adjacent to the hybridization region of the padlock probe. In some embodiments, hybridization of the primer to both the padlock probe and the target nucleic acid provides additional stability and/or specificity.

In some aspects, the present application provides polynucleotide probe sets comprising a control padlock probe, a detection padlock probe, and a connecting probe (e.g., an anchor) for amplification and identification of short regions of interest in target nucleic acids, such as SNPs in messenger RNA. The probe set permits highly sensitive and specific detection of short regions of interest such as SNPs in highly complex samples, for example in intact biological tissue containing numerous different mRNA sequences. As a result, image-based quantification of SNPs at subcellular resolution is enabled.

IV. Ligation

In some embodiments, provided herein are methods and compositions for performing a ligation of one or more polynucleotides. In some embodiments, the methods provided herein involve ligating together two of the polynucleotides, for instance the anchor and the splint. In some embodiments, the anchor and the splint are ligated in order to form a ligated anchor-splint. In some embodiments, provided herein are methods and compositions for performing a ligation that includes forming a tri-fork hybridization complex

In some embodiments, the methods provided herein involve ligating the 5′ and 3′ ends of one or more of the polynucleotides, for instance the control padlock probe and/or the detection padlock probe, in order to form a circular probe. In some aspects, the methods provided involve at least two ligation steps (e.g., ligation of the 5′ and 3′ ends of the control padlock probe and ligation of the 5′ and 3′ ends of the detection padlock probe). In some aspects the methods provided further comprise a third ligation step (e.g., ligation of a splint and anchor). In some cases, one or both of the ligation steps involve DNA-templated ligation, which may provide some advantages such as increased efficiency. For example, the first ligation step involves using a detection padlock probe as a template and the second ligation step involves using the ligated anchor-splint as a template.

In some embodiments, the provided methods involve ligating one or more polynucleotides that are part of a hybridization complex that comprises a target nucleic acid with a single nucleotide of interest. In some embodiments, the provided methods involve ligating the ends of a control padlock probe and ligating the ends of a detection padlock probe that are part of the hybridization complex. In some embodiments, the provided methods further comprise ligating an anchor and a splint. Exemplary anchors, splints, padlock probes, and target nucleic acids are described in Section III.

In some aspects, the ligation of the padlock probe is dependent on hybridization of the anchor and/or splint to HRa or HRd of the detection or control padlock probes, respectively. In some aspects, the ligation of the padlock probe is further dependent on the padlock probe hybridizing to the target nucleic acid, for instance on the detection padlock probe hybridizing to HR1′ and on the control padlock probe hybridizing to HR3′.

In some embodiments, the methods provided herein involve a ligation that includes forming a tri-fork hybridization complex between the splint (the first nucleic acid), the anchor (the second nucleic acid), and the detection padlock probe (the third nucleic acid). In some embodiments, the hybridization of the anchor to the target nucleic acid and hybridization of the detection padlock to the splint and the target nucleic acid as shown in FIGS. 2B and 2E may aid the ligation of the anchor and the splint by bringing terminal nucleotides of the polynucleotides in proximity. In some embodiments, the hybridization of the anchor to the target nucleic acid and hybridization of the detection padlock to the splint and the target nucleic acid as shown in FIGS. 2B and 2E may provide selectivity for the sequences to be ligated. In some embodiments, the hybridization of the anchor to the target nucleic acid and one end of the detection padlock probe and hybridization of the target nucleic acid to the other end of the detection padlock probe as shown in FIGS. 2C and 2F may aid the ligation of the ends of the detection padlock probe by bringing terminal nucleotides at the ends in proximity. In some embodiments, the ligation can include an enzymatic ligation, chemical ligation, template dependent ligation, and/or template independent ligation. In some embodiments, the nucleic acid molecules of the tri-fork hybridization complex may be oriented in various ways that allows hybridization to occur.

In some aspects, the ligation of the anchor and the splint is dependent on hybridization to each of the HRs contained in the target nucleic acid. In some aspects, the ligation of the anchor and the splint is dependent on the anchor hybridizing to HR2′ of the target nucleic acid and the padlock probe hybridizing to HR1′ of the target nucleic acid. In some aspects, the ligation of the anchor and splint is further dependent on the splint hybridizing to HRa of the detection padlock probe. In some aspects, the ligation of the detection padlock probe ends to circularize the padlock probe is dependent on hybridization of the padlock probe to HR1′ of the target nucleic acid and hybridization of the anchor to HR2′ of the target nucleic acid. In some cases, the ligation of the detection padlock probe ends to circularize the padlock probe is further dependent on the hybridization of HRb of the anchor to HRa of the detection padlock probe.

In some aspects, the ligation of the anchor and the splint is further dependent on the anchor hybridizing to the region of interest contained in the target nucleic acid. In some embodiments, base pairing between the region of interest contained in the target nucleic acid and the nucleotide complementary to the region of interest contained in the anchor promotes the end of the anchor to be in sufficient proximity for ligation to the splint. Thus, in some aspects, the ligation of the anchor and the splint is dependent on hybridization of the polynucleotides to each of HR2′, comprising the region of interest, HR1′, and HRa. In some embodiments, the ligated anchor-splint is used as primer and the circular probe as template for amplification in order to form an amplification product. In some aspects, the methods provided herein involve detecting the amplification product. In some aspects, the detection of the amplification product involves detecting the presence or absence of the amplification product. In some aspects, the methods provided herein involve sequencing all or a portion of the amplification product. Thus, in some aspects, the detection and/or sequencing of the amplification product is dependent on ligation of the anchor and the splint and the padlock probes and therefore on hybridization of the polynucleotides to each of HR2′, comprising the region of interest, HR1′, HR3′, and HRa and HRd. In certain aspects, this dependency provides the specificity by which the presence or identity of the single nucleotide of interest can be determined.

In some embodiments, ligation is performed under conditions permissive for specific hybridization of the polynucleotides to one another and/or to the target nucleic acid. In some embodiments, the ligation to form the ligated control probe, ligated detection probe, and optionally, the ligated anchor-splint is performed under conditions permissive for specific hybridization of the polynucleotides to one another and/or to the target nucleic acid. In some embodiments, the ligation to form the ligated control probe, ligated detection probe, and optionally, the ligated anchor-splint is performed under conditions permissive for specific hybridization of the control and detection padlock probes to the target nucleic acid. In some embodiments, the ligation to form the ligated control probe, ligated detection probe, and optionally, the ligated anchor-splint is performed under conditions permissive for specific hybridization of the anchor to the target nucleic acid. In some embodiments, the ligation to form the ligated control probe, ligated detection probe, and the ligated anchor-splint is performed under conditions permissive for specific hybridization of the splint to the detection padlock probe.

In some embodiments, the ligation to form the circular control and detection probes is performed under conditions permissive for specific hybridization of the control padlock probe and detection padlock probe to the target nucleic acid. In some embodiments, the ligation to form the circular control and detection probes is performed under conditions permissive for specific hybridization of the splint to the detection padlock probe.

In some embodiments, the 3′ end of the anchor and the 5′ end of the splint are ligated. In other embodiments, the 5′ end of the anchor and the 3′ end of the splint are ligated.

In some embodiments, the ligation of the control padlock probe and the ligation of the detection padlock probe are done sequentially. In some embodiments, the ligation of the control padlock probe and the ligation of the detection padlock probe are done simultaneously.

In some embodiments, the ligation of the anchor and the splint and the ligation of the detection padlock probe and the ligation of the control padlock probe are done sequentially. In some embodiments, the anchor and the splint are ligated before the detection padlock probe is ligated. In some embodiments, the anchor and the splint are ligated after the detection padlock probe is ligated. In other embodiments, the ligation of the anchor and the splint and the ligation of the detection padlock probe and the ligation of the control padlock probe are done simultaneously.

In some embodiments, the ligation of the anchor and the splint and/or the ligation of the ends of the padlock probes involves chemical ligation. In some embodiments, the ligation of the anchor and the splint and/or the ligation of the ends of the padlock probes involves template dependent ligation. In some embodiments, the ligation of the anchor and the splint and/or the ligation of the ends of the padlock probes involves template independent ligation. In some embodiments, the ligation of the anchor and the splint and/or the ligation of the ends of the padlock probes involves enzymatic ligation.

In some embodiments, the ligase is capable of ligating two polynucleotides at a junction where a third polynucleotide is juxtaposed without basepairing to one of the two nucleotides, for instance capable of ligating the anchor and the splint when a nucleotide of the anchor is juxtaposed to a nucleotide of the detection padlock probe, e.g., a nucleotide of HR1, without basepairing.

In some embodiments, the enzymatic ligation involves use of a ligase. In some aspects, the ligase used herein comprises an enzyme that is commonly used to join polynucleotides together or to join the ends of a single polynucleotide. An RNA ligase, a DNA ligase, or another variety of ligase can be used to ligate two nucleotide sequences together. Ligases comprise ATP-dependent double-strand polynucleotide ligases, NAD-i-dependent double-strand DNA or RNA ligases and single-strand polynucleotide ligases, for example any of the ligases described in EC 6.5.1.1 (ATP-dependent ligases), EC 6.5.1.2 (NAD+-dependent ligases), EC 6.5.1.3 (RNA ligases). Specific examples of ligases comprise bacterial ligases such as E. coli DNA ligase, Tth DNA ligase, Thermococcus sp. (strain 9° N) DNA ligase (9° N™ DNA ligase, New England Biolabs), Taq DNA ligase, Ampligase™ (Epicentre Biotechnologies) and phage ligases such as T3 DNA ligase, T4 DNA ligase and T7 DNA ligase and mutants thereof. In some embodiments, the ligase is a T4 RNA ligase. In some embodiments, the ligase is a splintR ligase. In some embodiments, the ligase is a single stranded DNA ligase. In some embodiments, the ligase is a T4 DNA ligase.

In some embodiments, hybridization of the ends of the padlock to the splint brings the two ends of the padlock probe into proximity for ligation. In some embodiments, the ligase is a ligase that has a DNA-splinted DNA ligase activity. In some embodiments, any or all of the anchor, splint, and padlock probe are DNA molecules. In some embodiments, the padlock probe serves as a DNA template substrate for the ligation of the splint to the anchor. In some embodiments, the splint serves as a DNA template substrate for the ligation of the padlock probe ends. In some embodiments, the ligated anchor-splint serves as a DNA template substrate for the ligation of the padlock probe ends.

In some embodiments, the 5′ and 3′ ends of the detection padlock probe and the 5′ and 3′ ends of the control padlock probe, and optionally the anchor and the splint may be ligated directly or indirectly. “Direct ligation” means that the ends of the polynucleotides hybridize immediately adjacently to one another to form a substrate for a ligase enzyme resulting in their ligation to each other (intramolecular ligation). Alternatively, “indirect” means that the ends of the polynucleotides hybridize non-adjacently to one another, i.e., separated by one or more intervening nucleotides or “gaps”. In some embodiments, said ends are not ligated directly to each other, but instead occurs either via the intermediacy of one or more intervening (so-called “gap” or “gap-filling” (oligo)nucleotides) or by the extension of the 3′ end of a probe to “fill” the “gap” corresponding to said intervening nucleotides (intermolecular ligation). In some cases, the gap of one or more nucleotides between the hybridized ends of the polynucleotides may be “filled” by one or more “gap” (oligo)nucleotide(s) which are complementary to the splint, padlock probe, or target nucleic acid. The gap may be a gap of 1 to 60 nucleotides or a gap of 1 to 40 nucleotides or a gap of 3 to 40 nucleotides. In specific embodiments, the gap may be a gap of about 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 or more nucleotides, of any integer (or range of integers) of nucleotides in between the indicated values. In some embodiments, the gap between said terminal regions may be filled by a gap oligonucleotide or by extending the 3′ end of a polynucleotide, for instance the padlock probe or anchor. In some cases, ligation involves ligating the ends of the probe to at least one gap (oligo)nucleotide, such that the gap (oligo)nucleotide becomes incorporated into the resulting polynucleotide.

In some embodiments, the ligation of the 5′ and 3′ ends of the control padlock probe does not require gap filling. In other embodiments, the ligation of the 5′ and 3′ ends of the control padlock probe is preceded by gap filling.

In some embodiments, the ligation of the 5′ and 3′ ends of the detection padlock probe does not require gap filling. In other embodiments, the ligation of the 5′ and 3′ ends of the detection padlock probe is preceded by gap filling.

In some embodiments, the ligation of the anchor and the splint is preceded by gap filling. In other embodiments, the ligation of the anchor and the splint does not require gap filling.

In some embodiments, ligation of the polynucleotides produces polynucleotides with melting temperature higher than that of unligated polynucleotides. Thus, in some aspects, ligation stabilizes the hybridization complex containing the ligated polynucleotides prior to subsequent steps, comprising amplification and detection. In some embodiments, the melting temperature of the ligated anchor-splint for hybridization to the target nucleic acid and the circular probe is higher than the T_(m) of the anchor for hybridization to the target nucleic acid. In some embodiments, the melting temperature of the ligated anchor-splint for hybridization to the target nucleic acid and the circular probe is higher than the T_(m) of the splint for hybridization to the circular probe.

In some aspects, a high fidelity ligase, such as a thermostable DNA ligase (e.g., a Taq DNA ligase), is used. Thermostable DNA ligases are active at elevated temperatures, allowing further discrimination by incubating the ligation at a temperature near the melting temperature (T_(m)) of the DNA strands. This selectively reduces the concentration of annealed mismatched substrates (expected to have a slightly lower T_(m) around the mismatch) over annealed fully base-paired substrates. Thus, high-fidelity ligation can be achieved through a combination of the intrinsic selectivity of the ligase active site and balanced conditions to reduce the incidence of annealed mismatched dsDNA.

V. Amplification

In some embodiments, the methods of the present disclosure comprise the step of amplifying one or more polynucleotides, for instance the padlock probe or a circular probe formed from the padlock probe. In some embodiments, the amplifying is achieved by performing rolling circle amplification (RCA). In some embodiments, the performing comprises contacting a target nucleic acid with a control padlock probe, a detection padlock probe, an anchor, and a primer to form a hybridization complex, e.g., using any of the control padlock probes, detection padlock probes, anchors, and primers described in Section III. In some embodiments, the performing comprises contacting a target nucleic acid with a control padlock probe, a detection padlock probe, an anchor, a splint, and a primer to form a hybridization complex, e.g., using any of the control padlock probes, detection padlock probes, anchors, splints, and primers described in Section III. In some embodiments, following ligation and circularization of the control and detection padlock probes to form circular control and detection probes, e.g., as performed using any of the exemplary methods described in Section IV, amplification is performed using the circular detection probe as template and the anchor or the ligated anchor-splint as a primer and using the circular control probe as a template and the primer as a primer. In other embodiments, a second primer that hybridizes to the detection padlock probe is added and used as such for amplification. In yet other embodiments, following ligation and circularization of the control and detection padlock probes to form circular control and detection probes, amplification is performed using the circular control probe as template and the anchor or the ligated anchor-splint as a primer and using the circular detection probe as a template and the primer as a primer.

In some embodiments, a removing step is performed to remove molecules that are not specifically hybridized to the target nucleic acid and/or the circular control and/or detection probes. In some embodiments, the removing step is performed to remove unligated probes (e.g., unligated anchor/splint probes). In some embodiments, the removing step is performed after ligation and prior to amplification. In some embodiments, the removing step is performed at a temperature lower than the melting temperature of the ligated anchor-splint for hybridization to the target nucleic acid and the circular probe. In some embodiments, the removing step is performed at a temperature higher than the melting temperature of the anchor for hybridization to the target nucleic acid. In some embodiments, the removing step is performed at a temperature higher than the melting temperature of the splint for hybridization to the circular probe. In some embodiments, the ligated anchor-splint remains specifically hybridized to the circular probe after the removing step. In some embodiments, the circular probe remains specifically hybridized to the target nucleic acid after the removing step. In some embodiments, the ligated anchor-splint remains specifically hybridized to the target nucleic acid after the removing step. In some embodiments, non-ligated splint molecules are removed. In some embodiments, non-ligated anchor molecules are removed.

In some embodiments, the amplification is performed at a temperature lower than the melting temperature of the ligated anchor-splint for hybridization to the target nucleic acid and the circular probe. In some embodiments, the amplification is performed at a temperature higher than the melting temperature of the anchor for hybridization to the target nucleic acid. In some embodiments, the amplification is performed at a temperature higher than the melting temperature of the splint for hybridization to the circular probe. In some embodiments, the amplification is performed at a temperature between or between about 20° C. and about 60° C. In some embodiments, the amplification is performed at a temperature between or between about 30° C. and about 40° C.

In some aspects, the amplification steps can be performed at a temperature that is lower than the T_(m) of hybridization of the HRs between the probe polynucleotides and target site on the target nucleic acid, at a temperature required for the amplification step. In some aspects, the amplification step, such as the rolling circle amplification (RCA) is performed at a temperature between at or about 25° C. and at or about 50° C., such as at or about 25° C., 27° C., 29° C., 31° C., 33° C., 35° C., 37° C., 39° C., 41° C., 43° C., 45° C., 47° C., or 49° C.

In some embodiments, upon addition of a DNA polymerase in the presence of appropriate dNTP precursors and other cofactors, the ligated anchor-splint or the primer is elongated to produce multiple copies of the circular template. This amplification step can utilize isothermal amplification or non-isothermal amplification. In some embodiments, after the formation of the hybridization complex and association of the amplification probe, the hybridization complex is rolling-circle amplified to generate a cDNA nanoball (i.e., amplicon) containing multiple copies of the cDNA. Techniques for rolling circle amplification (RCA) are known in the art such as linear RCA, a branched RCA, a dendritic RCA, or any combination thereof. (See, e.g., Baner et al, Nucleic Acids Research, 26:5073-5078, 1998; Lizardi et al, Nature Genetics 19:226, 1998; Mohsen et al., Acc Chem Res. 2016 Nov. 15; 49(11): 2540-2550; Schweitzer et al. Proc. Natl Acad. Sci. USA 97:101 13-1 19, 2000; Faruqi et al, BMC Genomics 2:4, 2000; Nallur et al, Nucl. Acids Res. 29:e118, 2001; Dean et al. Genome Res. 1 1:1095-1099, 2001; Schweitzer et al, Nature Biotech. 20:359-365, 2002; U.S. Pat. Nos. 6,054,274, 6,291,187, 6,323,009, 6,344,329 and 6,368,801), all of which are herein incorporated by reference in their entireties. Exemplary polymerases for use in RCA comprise DNA polymerase such phi29 (φ29) polymerase, Klenow fragment, Bacillus stearothermophilus DNA polymerase (BST), T4 DNA polymerase, T7 DNA polymerase, or DNA polymerase I. In some aspects, DNA polymerases that have been engineered or mutated to have desirable characteristics can be employed. In some embodiments, the polymerase is phi29 DNA polymerase.

In some aspects, during the amplification step, modified nucleotides can be added to the reaction to incorporate the modified nucleotides in the amplification product (e.g., nanoball). Exemplary of the modified nucleotides comprise amine-modified nucleotides. In some aspects, the modified nucleotides can be employed. In some aspects of the methods, for example, for anchoring or cross-linking of the generated amplification product (e.g., nanoball) to a scaffold, to cellular structures and/or to other amplification products (e.g., other nanoballs). In some aspects, the amplification products comprises a modified nucleotide, such as an amine-modified nucleotide. In some embodiments, the amine-modified nucleotide comprises an acrylic acid N-hydroxysuccinimide moiety modification. Examples of other amine-modified nucleotides comprise, but are not limited to, a 5-Aminoallyl-dUTP moiety modification, a 5-Propargylamino-dCTP moiety modification, a N6-6-Aminohexyl-dATP moiety modification, or a 7-Deaza-7-Propargylamino-dATP moiety modification.

In some aspects, the polynucleotides and/or amplification product (e.g., amplicon) can be anchored to a polymer matrix. For example, the polymer matrix can be a hydrogel. In some embodiments, one or more of the polynucleotide probe(s) can be modified to contain functional groups that can be used as an anchoring site to attach the polynucleotide probes and/or amplification product to a polymer matrix.

Exemplary modification and polymer matrix that can be employed in accordance with the provided embodiments comprise those described in, for example, U.S. Pat. No. 10,494,662, WO 2017/079406, U.S. Pat. No. 10,266,888, US 2016/0024555, US 2018/0251833 and U.S. Pat. No. 10,545,075, all of which are herein incorporated by reference in their entirety. In some examples, the scaffold also contains modifications or functional groups that can react with or incorporate the modifications or functional groups of the probe set or amplification product. In some examples, the scaffold can comprise oligonucleotides, polymers or chemical groups, to provide a matrix and/or support structures.

The amplification products may be immobilized within the matrix generally at the location of the nucleic acid being amplified, thereby creating a localized colony of amplicons. The amplification products may be immobilized within the matrix by steric factors. The amplification products may also be immobilized within the matrix by covalent or noncovalent bonding. In this manner, the amplification products may be considered to be attached to the matrix. By being immobilized to the matrix, such as by covalent bonding or cross-linking, the size and spatial relationship of the original amplicons is maintained. By being immobilized to the matrix, such as by covalent bonding or cross-linking, the amplification products are resistant to movement or unraveling under mechanical stress.

In some aspects, the amplification products are copolymerized and/or covalently attached to the surrounding matrix thereby preserving their spatial relationship and any information inherent thereto. For example, if the amplification products are those generated from DNA or RNA within a cell embedded in the matrix, the amplification products can also be functionalized to form covalent attachment to the matrix preserving their spatial information within the cell thereby providing a subcellular localization distribution pattern. In some embodiments, the provided methods involve embedding the one or more polynucleotide probe sets and/or the amplification products in the presence of hydrogel subunits to form one or more hydrogel-embedded amplification products. In some embodiments, the hydrogel-tissue chemistry described comprises covalently attaching nucleic acids to in situ synthesized hydrogel for tissue clearing, enzyme diffusion, and multiple-cycle sequencing while an existing hydrogel-tissue chemistry method cannot. In some embodiments, to enable amplification product embedding in the tissue-hydrogel setting, amine-modified nucleotides are comprised in the amplification step (e.g., RCA), functionalized with an acrylamide moiety using acrylic acid N-hydroxysuccinimide esters, and copolymerized with acrylamide monomers to form a hydrogel.

VI. Detection and Analysis

In some aspects, the provided methods involve analyzing, e.g., detecting or determining, one or more sequences present in the polynucleotides and/or in an amplification product, such as in an amplified padlock probe. In some embodiments, the analysis comprises determining the sequence of all or a portion of the amplification product. In some embodiments, the analysis comprises detecting a sequence present in the amplification product. In some embodiments, the sequence of all or a portion of the amplification product is indicative of the identity of a region of interest in a target nucleic acid.

In some embodiments, the methods comprise sequencing all or a portion of the amplification product, such as one or more barcode sequences present in the amplification product. In some embodiments, the analysis and/or sequence determination comprises sequencing all or a portion of the amplification product and/or in situ hybridization to the amplification product. In some embodiments, the sequencing step involves sequencing by hybridization, sequencing by ligation, and/or fluorescent in situ sequencing, hybridization-based in situ sequencing and/or wherein the in situ hybridization comprises sequential fluorescent in situ hybridization. In some embodiments, the analysis and/or sequence determination comprises detecting a polymer generated by a hybridization chain reaction (HCR) reaction, see e.g., US 2017/0009278, which is incorporated herein by reference in its entirety, for exemplary probes and HCR reaction components. In some embodiments, the detection or determination comprises hybridizing to the amplification product a detection oligonucleotide labeled with a fluorophore, an isotope, a mass tag, or a combination thereof. In some embodiments, the detection or determination comprises imaging the amplification product. In some embodiments, the target nucleic acid is an mRNA in a tissue sample, and the detection or determination is performed when the target nucleic acid and/or the amplification product is in situ in the tissue sample.

In some aspects, the provided methods comprise imaging the amplification product (e.g., amplicon) and/or one or more portions of the polynucleotides, for example, via binding of the detection probe and detecting the detectable label. In some embodiments, the detection probe comprises a detectable label that can be measured and quantitated. The terms “label” and “detectable label” comprise a directly or indirectly detectable moiety that is associated with (e.g., conjugated to) a molecule to be detected, e.g., a detectable probe, comprising, but not limited to, fluorophores, radioactive isotopes, fluorescers, chemiluminescers, enzymes, enzyme substrates, enzyme cofactors, enzyme inhibitors, chromophores, dyes, metal ions, metal sols, ligands (e.g., biotin or haptens) and the like.

The term “fluorophore” comprises a substance or a portion thereof that is capable of exhibiting fluorescence in the detectable range. Particular examples of labels that may be used in accordance with the provided embodiments comprise, but are not limited to phycoerythrin, Alexa dyes, fluorescein, YPet, CyPet, Cascade blue, allophycocyanin, Cy3, Cy5, Cy7, rhodamine, dansyl, umbelliferone, Texas red, luminol, acradimum esters, biotin, green fluorescent protein (GFP), enhanced green fluorescent protein (EGFP), yellow fluorescent protein (YFP), enhanced yellow fluorescent protein (EYFP), blue fluorescent protein (BFP), red fluorescent protein (RFP), firefly luciferase, Renilla luciferase, NADPH, beta-galactosidase, horseradish peroxidase, glucose oxidase, alkaline phosphatase, chloramphenical acetyl transferase, and urease.

Fluorescence detection in tissue samples can often be hindered by the presence of strong background fluorescence. “Autofluorescence” is the general term used to distinguish background fluorescence (that can arise from a variety of sources, including aldehyde fixation, extracellular matrix components, red blood cells, lipofuscin, and the like) from the desired immunofluorescence from the fluorescently labeled antibodies or probes. Tissue autofluorescence can lead to difficulties in distinguishing the signals due to fluorescent antibodies or probes from the general background. In some embodiments, a method disclosed herein utilizes one or more agents to reduce tissue autofluorescence, for example, Autofluorescence Eliminator (Sigma/EMD Millipore), TrueBlack Lipofuscin Autofluorescence Quencher (Biotium), MaxBlock Autofluorescence Reducing Reagent Kit (MaxVision Biosciences), and/or a very intense black dye (e.g., Sudan Black, or comparable dark chromophore).

In some embodiments, a detectable probe containing a detectable label can be used to detect one or more polynucleotide(s) and/or amplification products (e.g., amplicon) described herein. In some embodiments, the methods involve incubating the detectable probe containing the detectable label with the sample, washing unbound detectable probe, and detecting the label, e.g., by imaging. In some embodiments, the methods involve incubating the sample with a probe that comprises an overhang that does not hybridize to the amplification product and a sequence on the overhang hybridizes to another probe (e.g., a detectably labelled probe). In some aspects, the method comprises sequential hybridization of detectably labelled probes to generate a spatiotemporal signal signature or code that identifies or corresponds to the analyte (e.g., a single nucleotide of interest).

Examples of detectable labels comprise but are not limited to various radioactive moieties, enzymes, prosthetic groups, fluorescent markers, luminescent markers, bioluminescent markers, metal particles, protein-protein binding pairs and protein-antibody binding pairs. Examples of fluorescent proteins comprise, but are not limited to, yellow fluorescent protein (YFP), green fluorescence protein (GFP), cyan fluorescence protein (CFP), umbelliferone, fluorescein, fluorescein isothiocyanate, rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride and phycoerythrin.

Examples of bioluminescent markers comprise, but are not limited to, luciferase (e.g., bacterial, firefly and click beetle), luciferin, aequorin and the like. Examples of enzyme systems having visually detectable signals comprise, but are not limited to, galactosidases, glucorimidases, phosphatases, peroxidases and cholinesterases. Identifiable markers also comprise radioactive compounds such as ¹²⁵I, ³⁵S, 14^(C) or ³H. Identifiable markers are commercially available from a variety of sources.

Examples of fluorescent labels and nucleotides and/or polynucleotides conjugated to such fluorescent labels comprise those described in, for example, Hoagland, Handbook of Fluorescent Probes and Research Chemicals, Ninth Edition (Molecular Probes, Inc., Eugene, 2002); Keller and Manak, DNA Probes, 2nd Edition (Stockton Press, New York, 1993); Eckstein, editor, Oligonucleotides and Analogues: A Practical Approach (IRL Press, Oxford, 1991); and Wetmur, Critical Reviews in Biochemistry and Molecular Biology, 26:227-259 (1991), all of which are herein incorporated by reference in their entireties. In some embodiments, exemplary techniques and methods methodologies applicable to the provided embodiments comprise those described in, for example, U.S. Pat. Nos. 4,757,141, 5,151,507 and 5,091,519, all of which are herein incorporated by reference in their entireties. In some embodiments, one or more fluorescent dyes are used as labels for labeled target sequences, for example, as described in U.S. Pat. No. 5,188,934 (4,7-dichlorofluorescein dyes); U.S. Pat. No. 5,366,860 (spectrally resolvable rhodamine dyes); U.S. Pat. No. 5,847,162 (4,7-dichlororhodamine dyes); U.S. Pat. No. 4,318,846 (ether-substituted fluorescein dyes); U.S. Pat. No. 5,800,996 (energy transfer dyes); U.S. Pat. No. 5,066,580 (xanthine dyes); and U.S. Pat. No. 5,688,648 (energy transfer dyes), all of which are herein incorporated by reference in their entireties. Labelling can also be carried out with quantum dots, as described in U.S. Pat. Nos. 6,322,901, 6,576,291, 6,423,551, 6,251,303, 6,319,426, 6,426,513, 6,444,143, 5,990,479, 6,207,392, US 2002/0045045 and US 2003/0017264, all of which are herein incorporated by reference in their entireties. As used herein, the term “fluorescent label” comprises a signaling moiety that conveys information through the fluorescent absorption and/or emission properties of one or more molecules. Exemplary fluorescent properties comprise fluorescence intensity, fluorescence lifetime, emission spectrum characteristics and energy transfer.

Examples of commercially available fluorescent nucleotide analogues readily incorporated into nucleotide and/or polynucleotide sequences comprise, but are not limited to, Cy3-dCTP, Cy3-dUTP, Cy5-dCTP, Cy5-dUTP (Amersham Biosciences, Piscataway, N.J.), fluorescein-!2-dUTP, tetramethylrhodamine-6-dUTP, TEXAS RED™-5-dUTP, CASCADE BLUE™-7-dUTP, BODIPY TMFL-14-dUTP, BODIPY TMR-14-dUTP, BODIPY TMTR-14-dUTP, RHOD AMINE GREEN™-5-dUTP, OREGON GREENR™ 488-5-dUTP, TEXAS RED™-12-dUTP, BODIPY™ 630/650-14-dUTP, BODIPY™ 650/665-14-dUTP, ALEXA FLUOR™ 488-5-dUTP, ALEXA FLUOR™ 532-5-dUTP, ALEXA FLUOR™ 568-5-dUTP, ALEXA FLUOR™ 594-5-dUTP, ALEXA FLUOR™ 546-14-dUTP, fluorescein-12-UTP, tetramethylrhodamine-6-UTP, TEXAS RED™-5-UTP, mCherry, CASCADE BLUE™-7-UTP, BODIPY™ FL-14-UTP, BODIPY TMR-14-UTP, BODIPY™ TR-14-UTP, RHOD AMINE GREEN™-5-UTP, ALEXA FLUOR™ 488-5-UTP, and ALEXA FLUOR™ 546-14-UTP (Molecular Probes, Inc. Eugene, Oreg.). Methods are known for custom synthesis of nucleotides having other fluorophores (See, Henegariu et al. (2000) Nature Biotechnol. 18:345).

Other fluorophores available for post-synthetic attachment comprise, but are not limited to, ALEXA FLUOR™ 350, ALEXA FLUOR™ 532, ALEXA FLUOR™ 546, ALEXA FLUOR™ 568, ALEXA FLUOR™ 594, ALEXA FLUOR™ 647, BODIPY 493/503, BODIPY FL, BODIPY R6G, BODIPY 530/550, BODIPY TMR, BODIPY 558/568, BODIPY 558/568, BODIPY 564/570, BODIPY 576/589, BODIPY 581/591, BODIPY 630/650, BODIPY 650/665, Cascade Blue, Cascade Yellow, Dansyl, lissamine rhodamine B, Marina Blue, Oregon Green 488, Oregon Green 514, Pacific Blue, rhodamine 6G, rhodamine green, rhodamine red, tetramethyl rhodamine, Texas Red (available from Molecular Probes, Inc., Eugene, Oreg.), Cy2, Cy3.5, Cy5.5, and Cy7 (Amersham Biosciences, Piscataway, N.J.). FRET tandem fluorophores may also be used, comprising, but not limited to, PerCP-Cy5.5, PE-Cy5, PE-Cy5.5, PE-Cy7, PE-Texas Red, APC-Cy7, PE-Alexa dyes (610, 647, 680), and APC-Alexa dyes.

In some cases, metallic silver or gold particles may be used to enhance signal from fluorescently labeled nucleotide and/or polynucleotide sequences (Lakowicz et al. (2003) Bio Techniques 34:62).

Biotin, or a derivative thereof, may also be used as a label on a nucleotide and/or a polynucleotide sequence, and subsequently bound by a detectably labeled avidin/streptavidin derivative (e.g., phycoerythrin-conjugated streptavidin), or a detectably labeled anti-biotin antibody. Digoxigenin may be incorporated as a label and subsequently bound by a detectably labeled anti-digoxigenin antibody (e.g., fluoresceinated anti-digoxigenin). An aminoallyl-dUTP residue may be incorporated into a polynucleotide sequence and subsequently coupled to an N-hydroxy succinimide (NETS) derivatized fluorescent dye. In general, any member of a conjugate pair may be incorporated into a detection polynucleotide provided that a detectably labeled conjugate partner can be bound to permit detection. As used herein, the term antibody refers to an antibody molecule of any class, or any sub-fragment thereof, such as Fab.

Other suitable labels for a polynucleotide sequence may comprise fluorescein (FAM), digoxigenin, dinitrophenol (DNP), dansyl, biotin, bromodeoxyuridine (BrdU), hexahistidine (6×His), and phosphor-amino acids (e.g., P-tyr, P-ser, P-thr). In some embodiments the following hapten/antibody pairs are used for detection, in which each of the antibodies is derivatized with a detectable label: biotin/a-biotin, digoxigenin/a-digoxigenin, dinitrophenol (DNP)/a-DNP, 5-Carboxyfluorescein (FAM)/a-FAM.

In some embodiments, a nucleotide and/or an polynucleotide sequence can be indirectly labeled, especially with a hapten that is then bound by a capture agent, e.g., as disclosed in U.S. Pat. Nos. 5,344,757, 5,702,888, 5,354,657, 5,198,537 and 4,849,336, and PCT publication WO 91/17160. Many different hapten-capture agent pairs are available for use. Exemplary haptens comprise, but are not limited to, biotin, des-biotin and other derivatives, dinitrophenol, dansyl, fluorescein, Cy5, and digoxigenin. For biotin, a capture agent may be avidin, streptavidin, or antibodies. Antibodies may be used as capture agents for the other haptens (many dye-antibody pairs being commercially available, e.g., Molecular Probes, Eugene, Oreg.).

In some aspects, the detecting involves using detection methods such as flow cytometry; sequencing; probe binding and electrochemical detection; pH alteration; catalysis induced by enzymes bound to DNA tags; quantum entanglement; Raman spectroscopy; terahertz wave technology; and/or scanning electron microscopy. In some aspects, the flow cytometry is mass cytometry or fluorescence-activated flow cytometry. In some aspects, the detecting comprises performing microscopy, scanning mass spectrometry or other imaging techniques described herein. In such aspects, the detecting comprises determining a signal, e.g., a fluorescent signal.

In some aspects, the detection (comprising imaging) is carried out using any of a number of different types of microscopy, e.g., confocal microscopy, two-photon microscopy, light-field microscopy, intact tissue expansion microscopy, and/or CLARITY™-optimized light sheet microscopy (COLM).

In some embodiments, fluorescence microscopy is used for detection and imaging of the detection probe. In some aspects, a fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study properties of organic or inorganic substances. In fluorescence microscopy, a sample is illuminated with light of a wavelength which excites fluorescence in the sample. The fluoresced light, which is usually at a longer wavelength than the illumination, is then imaged through a microscope objective. Two filters may be used in this technique; an illumination (or excitation) filter which ensures the illumination is near monochromatic and at the correct wavelength, and a second emission (or barrier) filter which ensures none of the excitation light source reaches the detector. Alternatively, these functions may both be accomplished by a single dichroic filter. The “fluorescence microscope” comprises any microscope that uses fluorescence to generate an image, whether it is a more simple set up like an epifluorescence microscope, or a more complicated design such as a confocal microscope, which uses optical sectioning to get better resolution of the fluorescent image.

In some embodiments, confocal microscopy is used for detection and imaging of the detection probe. Confocal microscopy uses point illumination and a pinhole in an optically conjugate plane in front of the detector to eliminate out-of-focus signal. As only light produced by fluorescence very close to the focal plane can be detected, the image's optical resolution, particularly in the sample depth direction, is much better than that of wide-field microscopes. However, as much of the light from sample fluorescence is blocked at the pinhole, this increased resolution is at the cost of decreased signal intensity—so long exposures are often required. As only one point in the sample is illuminated at a time, 2D or 3D imaging requires scanning over a regular raster (i.e., a rectangular pattern of parallel scanning lines) in the specimen. The achievable thickness of the focal plane is defined mostly by the wavelength of the used light divided by the numerical aperture of the objective lens, but also by the optical properties of the specimen. The thin optical sectioning possible makes these types of microscopes particularly good at 3D imaging and surface profiling of samples. CLARITY™-optimized light sheet microscopy (COLM) provides an alternative microscopy for fast 3D imaging of large clarified samples. COLM interrogates large immunostained tissues, permits increased speed of acquisition and results in a higher quality of generated data.

Other types of microscopy that can be employed comprise bright field microscopy, oblique illumination microscopy, dark field microscopy, phase contrast, differential interference contrast (DIC) microscopy, interference reflection microscopy (also known as reflected interference contrast, or RIC), single plane illumination microscopy (SPIM), super-resolution microscopy, laser microscopy, electron microscopy (EM), Transmission electron microscopy (TEM), Scanning electron microscopy (SEM), reflection electron microscopy (REM), Scanning transmission electron microscopy (STEM) and low-voltage electron microscopy (LVEM), scanning probe microscopy (SPM), atomic force microscopy (ATM), ballistic electron emission microscopy (BEEM), chemical force microscopy (CFM), conductive atomic force microscopy (C-AFM), electrochemical scanning tunneling microscope (ECSTM), electrostatic force microscopy (EFM), fluidic force microscope (FluidFM), force modulation microscopy (FMM), feature-oriented scanning probe microscopy (FOSPM), kelvin probe force microscopy (KPFM), magnetic force microscopy (MFM), magnetic resonance force microscopy (MRFM), near-field scanning optical microscopy (NSOM) (or SNOM, scanning near-field optical microscopy, SNOM, Piezoresponse Force Microscopy (PFM), PSTM, photon scanning tunneling microscopy (PSTM), PTMS, photothermal microspectroscopy/microscopy (PTMS), SCM, scanning capacitance microscopy (SCM), SECM, scanning electrochemical microscopy (SECM), SGM, scanning gate microscopy (SGM), SHPM, scanning Hall probe microscopy (SHPM), SICM, scanning ion-conductance microscopy (SICM), SPSM spin polarized scanning tunneling microscopy (SPSM), SSRM, scanning spreading resistance microscopy (SSRM), SThM, scanning thermal microscopy (SThM), STM, scanning tunneling microscopy (STM), STP, scanning tunneling potentiometry (STP), SVM, scanning voltage microscopy (SVM), and synchrotron x-ray scanning tunneling microscopy (SXSTM), and intact tissue expansion microscopy (exM).

In some embodiments, sequencing can be performed in situ. In situ sequencing typically involves incorporation of a labeled nucleotide (e.g., fluorescently labeled mononucleotides or dinucleotides) in a sequential, template-dependent manner or hybridization of a labeled primer (e.g., a labeled random hexamer) to a nucleic acid template such that the identities (i.e., nucleotide sequence) of the incorporated nucleotides or labeled primer extension products can be determined, and consequently, the nucleotide sequence of the corresponding template nucleic acid. Aspects of in situ sequencing are described, for example, in Mitra et al., (2003) Anal. Biochem. 320, 55-65, and Lee et al., (2014) Science, 343(6177), 1360-1363. In addition, examples of methods and systems for performing in situ sequencing are described in US 2016/0024555, US 2019/0194709, and in U.S. Pat. Nos. 10,138,509, 10,494,662 and 10,179,932, all of which are herein incorporated by reference in their entireties. Exemplary techniques for in situ sequencing such as barcode detection schemes comprise those described in, but are not limited to, STARmap (described for example in Wang et al., (2018) Science, 361(6499) 5691), MERFISH (described for example in Moffitt, (2016) Methods in Enzymology, 572, 1-49), hybridization-based in situ sequencing (HybISS) (described for example in Gyllborg et al., Nucleic Acids Res (2020) 48(19):e112, and FISSEQ (described for example in US 2019/0032121). All of the aforementioned references are herein incorporated by reference in their entireties.

In some embodiments, sequencing can be performed by sequencing-by-synthesis (SBS). In some embodiments, a sequencing primer is complementary to sequences at or near the one or more barcode(s). In such embodiments, sequencing-by-synthesis can comprise reverse transcription and/or amplification in order to generate a template sequence from which a primer sequence can bind. Exemplary SBS methods comprise those described for example, but not limited to, US 2007/0166705, US 2006/0188901, U.S. Pat. No. 7,057,026, US 2006/0240439, US 2006/0281109, US 2011/005986, US 2005/0100900, U.S. Pat. No. 9,217,178, US 2009/0118128, US 2012/0270305, US 2013/0260372, and US 2013/0079232, all of which are herein incorporated by reference in their entireties.

In some embodiments, sequencing can be performed by sequential fluorescence hybridization (e.g., sequencing by hybridization). Sequential fluorescence hybridization can involve sequential hybridization of detection probes comprising an oligonucleotide and a detectable label.

In some embodiments, sequencing can be performed using single molecule sequencing by ligation. Such techniques utilize DNA ligase to incorporate oligonucleotides and identify the incorporation of such oligonucleotides. The oligonucleotides typically have different labels that are correlated with the identity of a particular nucleotide in a sequence to which the oligonucleotides hybridize. Aspects and features involved in sequencing by ligation are described, for example, in Shendure et al. Science (2005), 309: 1728-1732, and in U.S. Pat. Nos. 5,599,675; 5,750,341; 6,969,488; 6,172,218; and 6,306,597.

In some embodiments, the barcodes of the detection and control probes are targeted by detectably labeled detection oligonucleotides, such as fluorescently labeled oligonucleotides. In some embodiments, one or more decoding schemes are used to decode the signals, such as fluorescence, for sequence determination. In any of the embodiments herein, barcodes (e.g., primary and/or secondary barcode sequences) can be analyzed (e.g., detected or sequenced) using any suitable methods or techniques, comprising those described herein, such as RNA sequential probing of targets (RNA SPOTs), sequential fluorescent in situ hybridization (seqFISH), single-molecule fluorescent in situ hybridization (smFISH), multiplexed error-robust fluorescence in situ hybridization (MERFISH), hybridization-based in situ sequencing (HybISS), in situ sequencing, targeted in situ sequencing, fluorescent in situ sequencing (FISSEQ), or spatially-resolved transcript amplicon readout mapping (STARmap). In some embodiments, the methods provided herein comprise analyzing the barcodes by sequential hybridization and detection with a plurality of labelled probes (e.g., detection oligonucleotides). Exemplary decoding schemes are described in Eng et al., “Transcriptome-scale Super-Resolved Imaging in Tissues by RNA SeqFISH+,” Nature 568(7751):235-239 (2019); Chen et al., “Spatially resolved, highly multiplexed RNA profiling in single cells,” Science; 348(6233):aaa6090 (2015); Gyllborg et al., Nucleic Acids Res (2020) 48(19):e112; U.S. Pat. No. 10,457,980 B2; US 2016/0369329 A1; US 2021/0017587 A1; and US 2017/0220733 A1, all of which are incorporated by reference in their entirety. In some embodiments, these assays enable signal amplification, combinatorial decoding, and error correction schemes at the same time.

In some embodiments, nucleic acid hybridization can be used for sequencing. These methods utilize labeled nucleic acid decoder probes that are complementary to at least a portion of a barcode sequence. Multiplex decoding can be performed with pools of many different probes with distinguishable labels. Non-limiting examples of nucleic acid hybridization sequencing are described for example in U.S. Pat. No. 8,460,865, and in Gunderson et al., Genome Research 14:870-877 (2004).

In some embodiments, real-time monitoring of DNA polymerase activity can be used during sequencing. For example, nucleotide incorporations can be detected through fluorescence resonance energy transfer (FRET), as described for example in Levene et al., Science (2003), 299, 682-686, Lundquist et al., Opt. Lett. (2008), 33, 1026-1028, and Korlach et al., Proc. Natl. Acad. Sci. USA (2008), 105, 1176-1181, which are herein incorporated by reference in their entireties.

In some aspects, the analysis and/or sequence determination can be carried out at room temperature for best preservation of tissue morphology with low background noise and error reduction. In some embodiments, the analysis and/or sequence determination comprises eliminating error accumulation as sequencing proceeds.

In some embodiments, the analysis and/or sequence determination involves washing to remove unbound polynucleotides, thereafter revealing a fluorescent product for imaging.

A. Multiple Analytes

In some embodiments, a plurality of control padlock probes, detection padlock probes, anchors, primers, and optionally, splints can be used to target various regions of interest (e.g., various SNPs or SNP locations). In some embodiments, a method disclosed herein may be combined with methods analyzing one or more analytes other than nucleotide regions of interest.

In some aspects, the methods disclosed herein involve or can be combined with the use of one or more probes or probe sets that hybridize to a target nucleic acid, such as an RNA molecule. Exemplary probes or probe sets may be based on a padlock probe, a gapped padlock probe, a SNAIL (Splint Nucleotide Assisted Intramolecular Ligation) probe set, a PLAYR (Proximity Ligation Assay for RNA) probe set, a PLISH (Proximity Ligation in situ Hybridization) probe set, and RNA-templated ligation probes. The specific probe or probe set design can vary. In some embodiments, a primary probe (e.g., a DNA probe that directly binds to an RNA target) is amplified through rolling circle amplification, e.g., using a circular probe or a circularized probe from padlock ligation as a template. In some embodiments, the primary probes, such as a padlock probe or a probe set that comprises a padlock probe, contain one or more barcodes. In some embodiments, one or more barcodes are indicative of a sequence in the target nucleic acid, such as a region of interest (e.g., SNPs or point mutations), a dinucleotide sequence, a short sequence of about 5 nucleotides in length, or a sequence of any suitable length.

In some embodiments, provided herein is a probe or probe set capable of DNA-templated ligation, such as from a cDNA molecule. See, e.g., U.S. Pat. No. 8,551,710, which is hereby incorporated by reference in its entirety. In some embodiments, provided herein is a probe or probe set capable of RNA-templated ligation. See, e.g., U.S. Pat. Pub. 2020/0224244 which is hereby incorporated by reference in its entirety. In some embodiments, the probe set is a SNAIL probe set. See, e.g., U.S. Pat. Pub. 20190055594, which is hereby incorporated by reference in its entirety. In some embodiments, provided herein is a multiplexed proximity ligation assay. See, e.g., U.S. Pat. Pub. 20140194311 which is hereby incorporated by reference in its entirety. In some embodiments, provided herein is a probe or probe set capable of proximity ligation, for instance a proximity ligation assay for RNA (e.g., PLAYR) probe set. See, e.g., U.S. Pat. Pub. 20160108458, which is hereby incorporated by reference in its entirety.

In some embodiments, a circular probe can be indirectly hybridized to the target nucleic acid. In some embodiments, the circular construct is formed from a probe set capable of proximity ligation, for instance a proximity ligation in situ hybridization (PLISH) probe set. See, e.g., U.S. Pat. Pub. 2020/0224243 which is hereby incorporated by reference in its entirety.

In some embodiments, a protein analyte can be bound by one or more labelling agents capable of binding to or otherwise coupling to one or more features may be used to characterize cells and/or cell features (e.g., an antibody or antigen binding fragment thereof). In some embodiments, the labelling agents can include (e.g., are attached to) a reporter oligonucleotide that is indicative of the feature to which the labelling agent or portion thereof binds. For example, the reporter oligonucleotide may comprise a barcode sequence that permits identification of the labelling agent. In some examples, the labelling agent may include, but is not limited to, a protein, a peptide, an antibody (or an epitope binding fragment thereof), a lipophilic moiety (such as cholesterol), a cell surface receptor binding molecule, a receptor ligand, a small molecule, a bi-specific antibody, a bi-specific T-cell engager, a T-cell receptor engager, a B-cell receptor engager, a pro-body, an aptamer, a monobody, an affimer, a darpin, and a protein scaffold, or any combination thereof. In some embodiments, the nucleic acid molecule comprises a sequence corresponding to the labelling agent and therefore corresponds to (e.g., uniquely identifies) the protein analyte. In some embodiments, the reporter oligonucleotide (e.g., a reporter oligonucleotide) can comprise a nucleic acid molecule comprising one or more barcode sequences. Thus, the nucleic acid molecule of the labelling agent, like a nucleic acid analyte disclosed herein, can be analyzed using any methods disclosed herein. For example, the reporter oligonucleotide associated with the labelling agent that specifically recognizes a protein can be analyzed using in situ hybridization (e.g., sequential hybridization) and/or in situ sequencing (e.g., using padlock probes and rolling circle amplification of ligated padlock probes). Further, the reporter oligonucleotide and/or a complement thereof and/or a product (e.g., a hybridization product, a ligation product, an extension product (e.g., by a DNA or RNA polymerase), a replication product, a transcription/reverse transcription product, and/or an amplification product such as an RCA product) thereof can be analyzed.

In some embodiments, an analyte (a nucleic acid analyte or non-nucleic acid analyte) can be specifically bound by two labelling agents (e.g., antibodies) each of which is attached to a nucleic acid molecule (e.g., DNA) that can participate in ligation, replication, and sequence decoding reactions. For example, a proximity ligation reaction can include oligonucleotides attached to pairs of antibodies that can be joined by ligation if the antibodies have been brought in proximity to each oligonucleotide, e.g., by binding the same target protein (complex), and the DNA ligation products that form are then used to template PCR amplification, as described for example in Soderberg et al., Methods. (2008), 45(3): 227-32, the entire contents of which are incorporated herein by reference.

In some embodiments, an analyte is specifically bound by two primary antibodies, each of which is attached to a nucleic acid molecule (e.g., DNA). Each nucleic acid molecule serves as a splint for ligation of two probes which upon ligation form a circularized probe. Each of the two probes can comprise one or more barcode sequences. Further, one of the two probes may serve as a primer for rolling circle amplification (RCA) of the circularized probe while the other probe has an end that is blocked from primer extension. In some embodiments, an analyte is specifically bound by two primary antibodies, each of which in turn recognized by a secondary antibody attached to a nucleic acid molecule (e.g., DNA). Each nucleic acid molecule serves as a splint for ligation of two probes which upon ligation form a circularized probe. Each of the two probes can comprise one or more barcode sequences. Further, one of the two probes may serve as a primer for rolling circle amplification of the circularized probe while the other probe has an end that is blocked from primer extension. The nucleic acid molecules, circularized probes, and RCA products can be analyzed using any suitable method disclosed herein for in situ analysis.

In any of the embodiments disclosed herein, disclosed herein is a multiplexed assay where multiple targets (e.g., nucleic acids such as genes or RNA transcripts, or protein targets) are probed with multiple primary probes (e.g., padlock primary probes), and optionally multiple secondary probes hybridizing to the primary barcodes (or complementary sequences thereof) are all hybridized at once, followed by sequential secondary barcode detection and decoding of the signals. In some embodiments, detection of barcodes or subsequences of the barcode can occur in a cyclic manner.

In some embodiments, a method for analyzing a region of interest in a target nucleic acid is a multiplexed assay where multiple probes (e.g., padlock probes) are used to detect multiple regions of interest simultaneously (e.g., variations at the same location of a target nucleic acid and/or SNPs in various locations). In some embodiments, each of the multiple regions of interest can be associated with a control probe such that the control probe can provide an internal control for each region of interest to be detected. In some aspects, the control probe provides an internal control for performing the reactions required for detection (e.g., hybridization, ligation and/or amplification reactions). In some embodiments, one or more detections of one or more regions of interest may occur simultaneously. In some embodiments, one or more detections of one or more regions of interest may occur sequentially. In some embodiments, multiple padlock probes of the same padlock probe design are used to detect one or more regions of interest, using different barcodes associated with each region of interest. In some embodiments, multiple padlock probes of different padlock probe design are used to detect one or more regions of interest, using different barcodes (e.g., each barcode associated with a target nucleic acid or sequence thereof). In some embodiments, the one or more regions of interest are localized on the same molecule (e.g., RNA or DNA). In alternative embodiments, the one or more single nucleotides of interest are localized throughout the genome on different molecules.

VII. Compositions, Kits, and Systems

In some embodiments, disclosed herein is a composition that comprises a complex containing a target nucleic acid, a control complex comprising a control padlock probe, and a detection complex comprising a detection padlock probe and an anchor, e.g., any of the target nucleic acids, control padlock probes, detection padlock probes, and anchors described in Section III. In some embodiments, the complex further comprises a primer, e.g., as described in Section III. In some embodiments, the detection complex further comprises a splint, e.g., any of the splints described in Section III. In some embodiments, provided are kits comprising sets of polynucleotides, e.g., a set comprising associated control and detection probes.

In some embodiments, disclosed herein is a composition that comprises an amplification product containing an anchor portion, a splint portion, and monomeric units of a sequence complementary to a sequence (e.g., a sequence comprising HR1, a detection padlock barcode, and HRa, or a sequence comprising HR3, a control padlock barcode, and HRd) of a padlock probe. In some embodiments, the amplification product is formed using any of the target nucleic acids, padlock probes, splints, and anchors described in Section III and any of the amplification techniques described in Section V. In some embodiments, the amplification product further comprises a primer, e.g., any of the primers described in Section III. In some embodiments, the amplification product forms a DNA nanoball.

Also provided herein are kits, for example comprising one or more polynucleotides, e.g., any described in Section III, and reagents for performing the methods provided herein, for example reagents required for one or more steps comprising hybridization, ligation, amplification, detection, sequencing, and/or sample preparation as described herein. In some embodiments, the kit further comprises a target nucleic acid, e.g., any described in Section III. In some embodiments, any or all of the polynucleotides are DNA molecules. In some embodiments, the target nucleic acid is a messenger RNA molecule. In some embodiments, the kit further comprises a ligase, for instance for forming a ligated anchor-splint and a circular probe from the padlock probe. In some embodiments, the ligase has DNA-splinted DNA ligase activity. In some embodiments, the kit further comprises a polymerase, for instance for performing amplification of the padlock probe. In some embodiments, the polymerase is capable of using the ligated anchor-splint as a primer and the circular probe as a template for amplification, e.g., using any of the methods described in Section V. In some embodiments, the kit further comprises a primer for amplification.

The various components of the kit may be present in separate containers or certain compatible components may be pre-combined into a single container. In some embodiments, the kits further contain instructions for using the components of the kit to practice the provided methods.

In some embodiments, the kits can contain reagents and/or consumables required for performing one or more steps of the provided methods. In some embodiments, the kits contain reagents for fixing, embedding, and/or permeabilizing the biological sample. In some embodiments, the kits contain reagents, such as enzymes and buffers for ligation and/or amplification, such as ligases and/or polymerases. In some aspects, the kit can also comprise any of the reagents described herein, e.g., wash buffer and ligation buffer. In some embodiments, the kits contain reagents for detection and/or sequencing, such as barcode detection probes or detectable labels. In some embodiments, the kits optionally contain other components, for example nucleic acid primers, enzymes and reagents, buffers, nucleotides, modified nucleotides, reagents for additional assays.

VIII. Applications

In some aspects, the provided embodiments can be applied in an in situ method of analyzing nucleic acid sequences, such as an in situ transcriptomic analysis or in situ sequencing, for example from intact tissues or samples in which the spatial information has been preserved. In some aspects, the embodiments can be applied in an imaging or detection method for multiplexed nucleic acid analysis. In some aspects, the provided embodiments can be used to identify or detect regions of interest in target nucleic acids.

In some embodiments, the region of interest comprises a single-nucleotide polymorphism (SNP). In some embodiments, the region of interest comprises is a single-nucleotide variant (SNV). In some embodiments, the region of interest comprises a single-nucleotide substitution. In some embodiments, the region of interest comprises a point mutation. In some embodiments, the region of interest comprises a single-nucleotide insertion.

In some aspects, the embodiments can be applied in investigative and/or diagnostic applications, for example, for characterization or assessment of particular cell or a tissue from a subject. Applications of the provided method can comprise biomedical research and clinical diagnostics. For example, in biomedical research, applications comprise, but are not limited to, spatially resolved gene expression analysis for biological investigation or drug screening. In clinical diagnostics, applications comprise, but are not limited to, detecting gene markers such as disease, immune responses, bacterial or viral DNA/RNA for patient samples.

In some aspects, the embodiments can be applied to visualize the distribution of genetically encoded markers in whole tissue at subcellular resolution, for example, chromosomal abnormalities (inversions, duplications, translocations, etc.), loss of genetic heterozygosity, the presence of gene alleles indicative of a predisposition towards disease or good health, likelihood of responsiveness to therapy, or in personalized medicine or ancestry.

IX. Definitions

Unless defined otherwise, all terms of art, notations and other technical and scientific terms or terminology used herein are intended to have the same meaning as is commonly understood by one of ordinary skill in the art to which the claimed subject matter pertains. In some cases, terms with commonly understood meanings are defined herein for clarity and/or for ready reference, and the inclusion of such definitions herein should not necessarily be construed to represent a substantial difference over what is generally understood in the art.

The terms “polynucleotide,” “polynucleotide,” and “nucleic acid molecule”, used interchangeably herein, refer to polymeric forms of nucleotides of any length, either ribonucleotides or deoxyribonucleotides. Thus, this term comprises, but is not limited to, single-, double-, or multi-stranded DNA or RNA, genomic DNA, cDNA, DNA-RNA hybrids, or a polymer comprising purine and pyrimidine bases or other natural, chemically or biochemically modified, non-natural, or derivatized nucleotide bases. The backbone of the polynucleotide can comprise sugars and phosphate groups (as may typically be found in RNA or DNA), or modified or substituted sugar or phosphate groups.

“Hybridization” as used herein may refer to the process in which two single-stranded polynucleotides bind non-covalently to form a stable double-stranded polynucleotide. In one aspect, the resulting double-stranded polynucleotide can be a “hybrid” or “duplex.” “Hybridization conditions” typically include salt concentrations of approximately less than 1 M, often less than about 500 mM and may be less than about 200 mM. A “hybridization buffer” includes a buffered salt solution such as 5% SSPE, or other such buffers known in the art. Hybridization temperatures can be as low as 5° C., but are typically greater than 22° C., and more typically greater than about 30° C., and typically in excess of 37° C. Hybridizations are often performed under stringent conditions, i.e., conditions under which a sequence will hybridize to its target sequence but will not hybridize to other, non-complementary sequences. Stringent conditions are sequence-dependent and are different in different circumstances. For example, longer fragments may require higher hybridization temperatures for specific hybridization than short fragments. As other factors may affect the stringency of hybridization, including base composition and length of the complementary strands, presence of organic solvents, and the extent of base mismatching, the combination of parameters is more important than the absolute measure of any one parameter alone. Generally stringent conditions are selected to be about 5° C. lower than the T_(m) for the specific sequence at a defined ionic strength and pH. The melting temperature T_(m) can be the temperature at which a population of double-stranded nucleic acid molecules becomes half dissociated into single strands. Several equations for calculating the T_(m) of nucleic acids are well known in the art. As indicated by standard references, a simple estimate of the T_(m) value may be calculated by the equation, T_(m)=81.5+0.41 (% G+C), when a nucleic acid is in aqueous solution at 1 M NaCl (see e.g., Anderson and Young, Quantitative Filter Hybridization, in Nucleic Acid Hybridization (1985)). Other references (e.g., Allawi and SantaLucia, Jr., Biochemistry, 36:10581-94 (1997)) include alternative methods of computation which take structural and environmental, as well as sequence characteristics into account for the calculation of T_(m).

In general, the stability of a hybrid is a function of the ion concentration and temperature. Typically, a hybridization reaction is performed under conditions of lower stringency, followed by washes of varying, but higher, stringency. Exemplary stringent conditions include a salt concentration of at least 0.01 M to no more than 1 M sodium ion concentration (or other salt) at a pH of about 7.0 to about 8.3 and a temperature of at least 25° C. For example, conditions of 5×SSPE (750 mM NaCl, 50 mM sodium phosphate, 5 mM EDTA at pH 7.4) and a temperature of approximately 30° C. are suitable for allele-specific hybridizations, though a suitable temperature depends on the length and/or GC content of the region hybridized. In one aspect, “stringency of hybridization” in determining percentage mismatch can be as follows: 1) high stringency: 0.1×SSPE, 0.1% SDS, 65° C.; 2) medium stringency: 0.2×SSPE, 0.1% SDS, 50° C. (also referred to as moderate stringency); and 3) low stringency: 1.0×SSPE, 0.1% SDS, 50° C. It is understood that equivalent stringencies may be achieved using alternative buffers, salts and temperatures. For example, moderately stringent hybridization can refer to conditions that permit a nucleic acid molecule such as a probe to bind a complementary nucleic acid molecule. The hybridized nucleic acid molecules generally have at least 60% identity, including for example at least any of 70%, 75%, 80%, 85%, 90%, or 95% identity. Moderately stringent conditions can be conditions equivalent to hybridization in 50% formamide, 5×Denhardt's solution, 5×SSPE, 0.2% SDS at 42° C., followed by washing in 0.2×SSPE, 0.2% SDS, at 42° C. High stringency conditions can be provided, for example, by hybridization in 50% formamide, 5×Denhardt's solution, 5×SSPE, 0.2% SDS at 42° C., followed by washing in 0.1×SSPE, and 0.1% SDS at 65° C. Low stringency hybridization can refer to conditions equivalent to hybridization in 10% formamide, 5×Denhardt's solution, 6×SSPE, 0.2% SDS at 22° C., followed by washing in 1×SSPE, 0.2% SDS, at 37° C. Denhardt's solution contains 1% Ficoll, 1% polyvinylpyrolidone, and 1% bovine serum albumin (BSA). 20×SSPE (sodium chloride, sodium phosphate, ethylene diamide tetraacetic acid (EDTA)) contains 3M sodium chloride, 0.2M sodium phosphate, and 0.025 M EDTA. Other suitable moderate stringency and high stringency hybridization buffers and conditions are well known to those of skill in the art and are described, for example, in Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Press, Plainview, N.Y. (1989); and Ausubel et al., Short Protocols in Molecular Biology, 4th ed., John Wiley & Sons (1999).

Alternatively, substantial complementarity exists when an RNA or DNA strand will hybridize under selective hybridization conditions to its complement. Typically, selective hybridization will occur when there is at least about 65% complementary over a stretch of at least 14 to 25 nucleotides, preferably at least about 75%, more preferably at least about 90% complementary. See M. Kanehisa, Nucleic Acids Res. 12:203 (1984).

A “primer” used herein can be an oligonucleotide, either natural or synthetic, that is capable, upon forming a duplex with a polynucleotide template, of acting as a point of initiation of nucleic acid synthesis and being extended from its 3′ end along the template so that an extended duplex is formed. The sequence of nucleotides added during the extension process is determined by the sequence of the template polynucleotide. Primers usually are extended by a DNA polymerase.

“Ligation” may refer to the formation of a covalent bond or linkage between the termini of two or more nucleic acids, e.g., oligonucleotides and/or polynucleotides, in a template-driven reaction. The nature of the bond or linkage may vary widely and the ligation may be carried out enzymatically or chemically. As used herein, ligations are usually carried out enzymatically to form a phosphodiester linkage between a 5′ carbon terminal nucleotide of one oligonucleotide with a 3′ carbon of another nucleotide.

“Sequencing,” “sequence determination” and the like means determination of information relating to the nucleotide base sequence of a nucleic acid. Such information may include the identification or determination of partial as well as full sequence information of the nucleic acid. Sequence information may be determined with varying degrees of statistical reliability or confidence. In one aspect, the term includes the determination of the identity and ordering of a plurality of contiguous nucleotides in a nucleic acid. “High throughput digital sequencing” or “next generation sequencing” means sequence determination using methods that determine many (typically thousands to billions) of nucleic acid sequences in an intrinsically parallel manner, i.e. where DNA templates are prepared for sequencing not one at a time, but in a bulk process, and where many sequences are read out preferably in parallel, or alternatively using an ultra-high throughput serial process that itself may be parallelized. Such methods include but are not limited to pyrosequencing (for example, as commercialized by 454 Life Sciences, Inc., Branford, Conn.); sequencing by ligation (for example, as commercialized in the SOLiD™ technology, Life Technologies, Inc., Carlsbad, Calif.); sequencing by synthesis using modified nucleotides (such as commercialized in TruSeq™ and HiSeg™ technology by Illumina, Inc., San Diego, Calif.; HeliScope™ by Helicos Biosciences Corporation, Cambridge, Mass.; and PacBio RS by Pacific Biosciences of California, Inc., Menlo Park, Calif.), sequencing by ion detection technologies (such as Ion Torrent™ technology, Life Technologies, Carlsbad, Calif.); sequencing of DNA nanoballs (Complete Genomics, Inc., Mountain View, Calif.); nanopore-based sequencing technologies (for example, as developed by Oxford Nanopore Technologies, LTD, Oxford, UK), and like highly parallelized sequencing methods.

“SNP” or “single nucleotide polymorphism” may include a genetic variation between individuals; e.g., a single nitrogenous base position in the DNA of organisms that is variable. SNPs are found across the genome; much of the genetic variation between individuals is due to variation at SNP loci, and often this genetic variation results in phenotypic variation between individuals. SNPs for use in the present disclosure and their respective alleles may be derived from any number of sources, such as public databases (U.C. Santa Cruz Human Genome Browser Gateway (genome.ucsc.edu/cgi-bin/hgGateway) or the NCBI db SNP web site (www.ncbi.nlm.nih gov/SNP/), or may be experimentally determined as described in U.S. Pat. No. 6,969,589; and US Pub. No. 2006/0188875 entitled “Human Genomic Polymorphisms,” all of which are herein incorporated by reference in their entireties. Although the use of SNPs is described in some of the embodiments presented herein, it will be understood that other biallelic or multi-allelic genetic markers may also be used. A biallelic genetic marker is one that has two polymorphic forms, or alleles. As mentioned above, for a biallelic genetic marker that is associated with a trait, the allele that is more abundant in the genetic composition of a case group as compared to a control group is termed the “associated allele,” and the other allele may be referred to as the “unassociated allele.” Thus, for each biallelic polymorphism that is associated with a given trait (e.g., a disease or drug response), there is a corresponding associated allele. Other biallelic polymorphisms that may be used with the methods presented herein include, but are not limited to multinucleotide changes, insertions, deletions, and translocations. It will be further appreciated that references to DNA herein may include genomic DNA, mitochondrial DNA, episomal DNA, and/or derivatives of DNA such as amplicons, RNA transcripts, cDNA, DNA analogs, etc. The polymorphic loci that are screened in an association study may be in a diploid or a haploid state and, ideally, would be from sites across the genome.

“Multiplexing” or “multiplex assay” herein may refer to an assay or other analytical method in which the presence and/or amount of multiple targets, e.g., multiple nucleic acid target sequences, can be assayed simultaneously by using more than one capture probe conjugate, each of which has at least one different detection characteristic, e.g., fluorescence characteristic (for example excitation wavelength, emission wavelength, emission intensity, FWHM (full width at half maximum peak height), or fluorescence lifetime) or a unique nucleic acid or protein sequence characteristic.

The term “about” as used herein refers to the usual error range for the respective value readily known to the skilled person in this technical field. Reference to “about” a value or parameter herein comprises (and describes) embodiments that are directed to that value or parameter per se.

As used herein, the singular forms “a,” “an,” and “the” comprise plural referents unless the context clearly dictates otherwise. For example, “a” or “an” means “at least one” or “one or more.”

Throughout this disclosure, various aspects of the claimed subject matter are presented in a range format. It should be understood that the description in range format is merely for convenience and brevity and should not be construed as an inflexible limitation on the scope of the claimed subject matter. Accordingly, the description of a range should be considered to have specifically disclosed all the possible sub-ranges as well as individual numerical values within that range. For example, where a range of values is provided, it is understood that each intervening value, between the upper and lower limit of that range and any other stated or intervening value in that stated range is encompassed within the claimed subject matter. The upper and lower limits of these smaller ranges may independently be comprised in the smaller ranges, and are also encompassed within the claimed subject matter, subject to any specifically excluded limit in the stated range. Where the stated range comprises one or both of the limits, ranges excluding either or both of those comprised limits are also comprised in the claimed subject matter. This applies regardless of the breadth of the range.

Use of ordinal terms such as “first”, “second”, “third”, etc., in the claims to modify a claim element does not by itself connote any priority, precedence, or order of one claim element over another or the temporal order in which acts of a method are performed, but are used merely as labels to distinguish one claim element having a certain name from another element having a same name (but for use of the ordinal term) to distinguish the claim elements. Similarly, use of a), b), etc., or i), ii), etc. does not by itself connote any priority, precedence, or order of steps in the claims. Similarly, the use of these terms in the specification does not by itself connote any required priority, precedence, or order.

EXAMPLE Example 1: Hybridization, Amplification, and Detection

Exemplary probe sets such as any one of the probe sets shown in FIGS. 2A-2F are hybridized, amplified, detected and sequenced for in situ detection of short regions of interest, such as SNPs, in a biological sample, such as a tissue section.

A probe set mixture is heated, cooled down to room temperature, and incubated with a thin tissue section sample and hybridization buffer for hybridization of the probe sets to target nucleic acid (e.g., mRNAs) in the sample. Hybridization is performed at a temperature optimized for the detection probe, anchor, and/or control padlock. In some cases, the optimized hybridization condition is a temperature that is below the melting temperature (T_(m)) of each of the hybridizations between HR1/HR1′, HR2/HR2′, and HR3/HR3′.

The sample is then washed and incubated at room temperature with a T4 DNA ligase for ligation of the 5′ and 3′ ends of the control and detection padlock probes to form circular probes.

In some examples shown in FIG. 2A, 2C, 2D and FIG. 2F, ligation of the detection padlock probe ends depends on hybridization between an interrogatory region and the region of interest, and ligation of the control padlock probe provides a positive control for hybridization of the control padlock and anchor to regions (HR2′ and HR3′) adjacent to the hybridization region HR1′ of the detection padlock probe.

In some examples shown in FIGS. 2B and 2E, the ligation step comprises ligation of the anchors to the splints. In some examples, a stringent wash (e.g., conditions including temperature of about 5° C. less that the T_(m) of a strand of the duplex and low monovalent salt concentration, e.g., less than 0.2 M, or less than 0.1 M) is then performed at a temperature that is lower than the T_(m) of the ligated anchor-splints for hybridization to the target mRNAs and the circular probes, and higher than the T_(m) of the unligated splints for hybridization to the circular probes. In some examples, ligation of the anchor and splint is required to stabilize hybridization of the splint and the detection padlock probe in order to allow ligation of the detection padlock probe. In some examples, ligation of the anchor and the splint is required to stabilize hybridization of the anchor-splint to the target nucleic acid and to either padlock probe in order to allow ligation of the detection and/or control padlock probes. After the stringent wash, the sample is then incubated with a rolling-circle amplification (RCA) mixture containing a Phi29 DNA polymerase and dNTP at approximately 30° C. for RCA of the circular probes. In some instances, modified nucleotide bases (e.g., 5-(3-aminoallyl)-dUTP) are also comprised in the RCA mixture.

In an exemplary outcome of the ligation, stringent wash, and RCA steps applied to the complex illustrated in FIGS. 2B and 2E, probe sets that bind to a target SNP will have their anchors ligated to splints, and the ligated anchor-splints will remain stably hybridized after the stringent wash at high temperature is performed. In some examples, ligation of the anchor and splint is required to stabilize hybridization of the splint to the detection padlock probe and/or hybridization of the anchor to the control padlock probe, and is thus required to allow ligation of the ends of the detection padlock probe and/or control padlock probe. In this case, productive RCA depends on ligation of the anchor and the splint and on the ligation of the detection padlock probe and/or of the control padlock probe. On the other hand, probe sets that do not bind to a target SNP will not have their anchors ligated to splints, and the unligated splints will be washed away by the stringent wash, optionally at high temperature. In this case, ligation of the detection padlock probe and/or RCA priming will not be achieved, and the circular probe will not be amplified.

For single-SNP detection, fluorescently labeled oligonucleotides complementary to a portion of the amplified circular probes (e.g., of the RCA product, a barcode contained therein, or a secondary probe attached thereto) are incubated with the sample, and after a wash step, images are obtained. Multiple cycles of hybridization are performed and images are acquired each cycle using a confocal microscope. For each detection padlock probe, a control padlock probe comprising a control barcode is also detected using fluorescently labeled oligonucleotides. If the nucleotide of interest does not match (i.e., basepair with) the interrogatory nucleotide of the detection padlock probe, then the control padlock probe but not the detection padlock probe will be circularized and amplified by rolling circle amplification. Thus, mismatch between the nucleotide of interest and the interrogatory interest results in detection of the control padlock barcode only. If the nucleotide of interest matches (i.e., basepairs with) the interrogatory nucleotide of the detection padlock probe, then both the detection padlock barcode and the control padlock barcode is detected. Probe sets targeting various regions of interest within or across genes can be sequentially or simultaneously provided, processed, and detected as described above.

The present disclosure is not intended to be limited in scope to the particular disclosed embodiments, which are provided, for example, to illustrate various aspects of the present disclosure. Various modifications to the compositions and methods described will become apparent from the description and teachings herein. Such variations may be practiced without departing from the true scope and spirit of the disclosure and are intended to fall within the scope of the present disclosure. 

1. A method for analyzing a region of interest in a target nucleic acid, the method comprising: (i) contacting a target nucleic acid with a control padlock probe and a detection complex comprising a detection padlock probe, wherein the detection complex comprises a sequence complementary to the region of interest and the control padlock probe comprises a sequence complementary to the target nucleic acid at a region that is different from the region of interest, (ii) forming a circularized detection padlock probe and/or a circularized control padlock probe; (iii) generating one or more amplification products using the circularized detection padlock probe and/or the circularized control padlock probe as template; and (iv) detecting the presence or absence of the amplification product of the detection padlock probe and/or the amplification product of the circularized control padlock probe.
 2. The method of claim 1, wherein the presence of the amplification product of the detection padlock probe and the presence of the amplification product of the control padlock probe indicates the presence of the region of interest in the target nucleic acid.
 3. The method of claim 1, wherein the absence of the amplification product of the detection padlock probe and the presence of the amplification products of the control padlock probe indicates the absence of the region of interest in the target nucleic acid.
 4. The method of claim 1, wherein formation of the circularized control padlock probe is not dependent on the detection padlock probe hybridizing to the target nucleic acid.
 5. The method of claim 1, wherein formation of the circularized control padlock probe is not dependent on formation of the circularized detection padlock probe.
 6. The method of claim 1, wherein the detection padlock probe comprises the sequence complementary to the region of interest.
 7. The method of claim 1, wherein the circularized detection padlock probe is formed using the target nucleic acid as template.
 8. (canceled)
 9. The method of claim 1, wherein the detection complex further comprises an anchor.
 10. (canceled)
 11. The method of claim 9, wherein the anchor comprises a sequence complementary to the target nucleic acid at a region that is different from the region of interest.
 12. The method of claim 9, wherein the anchor comprises a sequence that hybridizes to the detection padlock probe and/or a sequence that hybridizes to the control padlock probe. 13-16. (canceled)
 17. The method of claim 9, wherein ends of the control padlock probe are ligated using the anchor as template to form the circularized control padlock. 18-22. (canceled)
 23. The method of claim 9, wherein the detection padlock probe, the anchor, and the control padlock probe hybridize to adjacent sequences of the target nucleic acid. 24-27. (canceled)
 28. The method of claim 9, wherein the anchor is used as a primer for rolling circle amplification of the circularized detection padlock probe and a separate primer is provided for rolling circle amplification of the circularized control padlock probe, or wherein the anchor is used as a primer for rolling circle amplification of the circularized control padlock probe and a separate primer is provided for rolling circle amplification of the circularized detection padlock probe.
 29. (canceled)
 30. The method of claim 1, wherein the region of interest comprises a single nucleotide of interest, an alternatively spliced region, a deletion, and/or a frameshift. 31-34. (canceled)
 35. The method of claim 1, wherein the detection complex is a first detection complex and the detection padlock probe is a first detection padlock probe, the first detection complex comprises a sequence complementary to a first sequence of the region of interest, and the method further comprises: contacting the target nucleic acid with a second detection complex comprising a second detection padlock probe, wherein the second detection complex comprises a sequence complementary to a second sequence of the region of interest, and wherein the first and second sequences of the region of interest are different; forming a circularized second detection padlock probe; generating one or more amplification products using the circularized second detection padlock probe; and detecting the presence or absence of the amplification product of the second detection padlock probe. 36-37. (canceled)
 38. The method of claim 35, wherein the second sequence of the region of interest is a variant of the first sequence of the region of interest, or vice versa.
 39. (canceled)
 40. The method of claim 35, wherein the first detection complex comprises a first barcode sequence corresponding to the first sequence of the region of interest, the second detection complex comprises a second barcode sequence corresponding to the second sequence of the region of interest, and the first and second barcode sequences are different. 41-56. (canceled)
 57. The method of claim 1, wherein the detecting step comprises determining a sequence of the amplification product of the detection padlock probe and/or the amplification product of the circularized control padlock probe and/or in situ hybridization to the one or more amplification products.
 58. (canceled)
 59. The method of claim 57, wherein a barcode sequence in the amplification product of the detection padlock probe and/or the amplification product of the circularized control padlock probe is determined. 60-85. (canceled)
 86. The method of claim 1, wherein the target nucleic acid is in a biological sample, and wherein the region of interest is analyzed in situ in the biological sample. 87-100. (canceled) 